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THE PLANT CELL, Vol 7, Issue 1 65-74, Copyright © 1995 by American Society of Plant Biologists
The Significance of Microspore Division and Division Symmetry for Vegetative Cell-Specific Transcription and Generative Cell Differentiation
C. Eady, K. Lindsey and D. Twell
Department of Botany, University of Leicester, Leicester LE1 7RH, United Kingdom
The significance of the onset and symmetry of pollen mitosis I (PMI) for
the subsequent differentiation of the vegetative and generative cells was
investigated by the in vitro maturation of isolated microspores of
transgenic tobacco. Free uninucleate microspores of transgenic plants
harboring the vegetative cell (VC)-specific late anther tomato lat52
promoter fused to the [beta]-glucuronidase (gus) gene showed normal
asymmetric cell division at PMI and activated the lat52 promoter
specifically in the nascent VC during in vitro maturation. In vitro
maturation in the presence of high levels of colchicine effectively blocked
PMI, resulting in the formation of uninucleate pollen grains in which the
lat52 promoter was activated. Furthermore, matured uninucleate pollen
grains were capable of germination and pollen tube growth despite the
absence of a functional generative cell (GC). Lower levels of colchicine
induced symmetric division at PMI, producing two similar daughter cells in
which typical GC chromatin condensation was prevented. Similar cultures of
transgenic microspores harboring the lat52 promoter driving the expression
of a nuclear-targeted GUS fusion protein showed that lat52 promoter
activation occurred in both symmetric daughter cells. These results
directly demonstrate that division asymmetry at PMI is essential for
correct GC differentiation and that activation of VC-specific transcription
and functional VC maturation may be uncoupled from cytokinesis at PMI.
These results are discussed in relation to models proposed to account for
the role and distribution of factors controlling the differing fates of the
vegetative and generative cells.
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