Plant Cell Applied BioSystems, Inc.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Eady, C.
Right arrow Articles by Twell, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Eady, C.
Right arrow Articles by Twell, D.
Agricola
Right arrow Articles by Eady, C.
Right arrow Articles by Twell, D.

THE PLANT CELL, Vol 7, Issue 1 65-74, Copyright © 1995 by American Society of Plant Biologists

RESEARCH ARTICLES

The Significance of Microspore Division and Division Symmetry for Vegetative Cell-Specific Transcription and Generative Cell Differentiation

C. Eady, K. Lindsey and D. Twell
Department of Botany, University of Leicester, Leicester LE1 7RH, United Kingdom

The significance of the onset and symmetry of pollen mitosis I (PMI) for the subsequent differentiation of the vegetative and generative cells was investigated by the in vitro maturation of isolated microspores of transgenic tobacco. Free uninucleate microspores of transgenic plants harboring the vegetative cell (VC)-specific late anther tomato lat52 promoter fused to the [beta]-glucuronidase (gus) gene showed normal asymmetric cell division at PMI and activated the lat52 promoter specifically in the nascent VC during in vitro maturation. In vitro maturation in the presence of high levels of colchicine effectively blocked PMI, resulting in the formation of uninucleate pollen grains in which the lat52 promoter was activated. Furthermore, matured uninucleate pollen grains were capable of germination and pollen tube growth despite the absence of a functional generative cell (GC). Lower levels of colchicine induced symmetric division at PMI, producing two similar daughter cells in which typical GC chromatin condensation was prevented. Similar cultures of transgenic microspores harboring the lat52 promoter driving the expression of a nuclear-targeted GUS fusion protein showed that lat52 promoter activation occurred in both symmetric daughter cells. These results directly demonstrate that division asymmetry at PMI is essential for correct GC differentiation and that activation of VC-specific transcription and functional VC maturation may be uncoupled from cytokinesis at PMI. These results are discussed in relation to models proposed to account for the role and distribution of factors controlling the differing fates of the vegetative and generative cells.


This article has been cited by other articles:


Home page
 Plant CellHome page
L. Brownfield, S. Hafidh, A. Durbarry, H. Khatab, A. Sidorova, P. Doerner, and D. Twell
Arabidopsis DUO POLLEN3 Is a Key Regulator of Male Germline Development and Embryogenesis
PLANT CELL, July 1, 2009; 21(7): 1940 - 1956.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Z. Chen, S. Hafidh, S. H. Poh, D. Twell, and F. Berger
Proliferation and cell fate establishment during Arabidopsis male gametogenesis depends on the Retinoblastoma protein
PNAS, April 28, 2009; 106(17): 7257 - 7262.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Borg, L. Brownfield, and D. Twell
Male gametophyte development: a molecular perspective
J. Exp. Bot., April 1, 2009; 60(5): 1465 - 1478.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
R. Matsushima, Y. Hamamura, T. Higashiyama, S.-i. Arimura, Sodmergen, N. Tsutsumi, and W. Sakamoto
Mitochondrial Dynamics in Plant Male Gametophyte Visualized by Fluorescent Live Imaging
Plant Cell Physiol., July 1, 2008; 49(7): 1074 - 1083.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. Liu, Y. Zhang, G. Qin, T. Tsuge, N. Sakaguchi, G. Luo, K. Sun, D. Shi, S. Aki, N. Zheng, et al.
Targeted Degradation of the Cyclin-Dependent Kinase Inhibitor ICK4/KRP6 by RING-Type E3 Ligases Is Essential for Mitotic Cell Cycle Progression during Arabidopsis Gametogenesis
PLANT CELL, June 1, 2008; 20(6): 1538 - 1554.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Durbarry, I. Vizir, and D. Twell
Male Germ Line Development in Arabidopsis. duo pollen Mutants Reveal Gametophytic Regulators of Generative Cell Cycle Progression
Plant Physiology, January 1, 2005; 137(1): 297 - 307.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
Z. A Wilson and C. Yang
Plant gametogenesis: conservation and contrasts in development
Reproduction, November 1, 2004; 128(5): 483 - 492.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
K.J. Kasha, T.C. Hu, R. Oro, E. Simion, and Y.S. Shim
Nuclear fusion leads to chromosome doubling during mannitol pretreatment of barley (Hordeum vulgare L.) microspores
J. Exp. Bot., June 1, 2001; 52(359): 1227 - 1238.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. K. Park and D. Twell
Novel Patterns of Ectopic Cell Plate Growth and Lipid Body Distribution in the Arabidopsis gemini pollen1 Mutant
Plant Physiology, June 1, 2001; 126(2): 899 - 909.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Souter and K. Lindsey
Polarity and signalling in plant embryogenesis
J. Exp. Bot., June 1, 2000; 51(347): 971 - 983.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
R. C. Brown and B. E. Lemmon
The cytoskeleton and polarization during pollen development in Carex blanda (Cyperaceae)
Am. J. Botany, January 1, 2000; 87(1): 1 - 11.
[Abstract] [Full Text]

Home page
 J. Cell Sci.Home page
K Boudonck, L Dolan, and P. Shaw
Coiled body numbers in the Arabidopsis root epidermis are regulated by cell type, developmental stage and cell cycle parameters
J. Cell Sci., June 14, 1999; 111(24): 3687 - 3694.
[Abstract] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Xu, I. Swoboda, P. L. Bhalla, and M. B. Singh
Male gametic cell-specific gene expression in flowering plants
PNAS, March 2, 1999; 96(5): 2554 - 2558.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
K Gallagher and L. Smith
discordia mutations specifically misorient asymmetric cell divisions during development of the maize leaf epidermis
Development, January 10, 1999; 126(20): 4623 - 4633.
[Abstract] [PDF]

Home page
 DevelopmentHome page
S. Park, R Howden, and D Twell
The Arabidopsis thaliana gametophytic mutation gemini pollen1 disrupts microspore polarity, division asymmetry and pollen cell fate
Development, January 10, 1998; 125(19): 3789 - 3799.
[Abstract] [PDF]

Home page
 DevelopmentHome page
M Spielman, D Preuss, F. Li, W. Browne, R. Scott, and H. Dickinson
TETRASPORE is required for male meiotic cytokinesis in Arabidopsis thaliana
Development, January 7, 1997; 124(13): 2645 - 2657.
[Abstract] [PDF]

Home page
 DevelopmentHome page
Y. Chen and S McCormick
sidecar pollen, an Arabidopsis thaliana male gametophytic mutant with aberrant cell divisions during pollen development
Development, January 10, 1996; 122(10): 3243 - 3253.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1995 by the American Society of Plant Biologists