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THE PLANT CELL, Vol 7, Issue 10 1545-1554, Copyright © 1995 by American Society of Plant Biologists
Phloem Loading by the PmSUC2 Sucrose Carrier from Plantago major Occurs into Companion Cells
R. Stadler, J. Brandner, A. Schulz, M. Gahrtz and N. Sauer
Lehrstuhl fur Zellbiologie und Pflanzenphysiologie, Universitat Regensburg, D-93040 Regensburg, Germany
High levels of mRNA for the sucrose-H+ symporter PmSUC2 have been found in
the vascular bundles of petioles from Plantago major. The possible role of
PmSUC2 in phloem loading was studied with antiserum raised against the
recombinant PmSUC2 protein. This antiserum labeled a single 35-kD protein
band in detergent extracts of P. major vascular bundles. It showed no
cross-reaction with the P. major sucrose carrier PmSUC1, which was tested
with detergent extracts from plasma membranes of transgenic yeast strains
containing either the P. major sucrose transporter PmSUC1 or PmSUC2. The
antiserum was used to determine the site of PmSUC2 expression in leaves,
petioles, and roots of P. major. In cross-sections and longitudinal
sections, the PmSUC2 protein was found in only one single cell type. These
cells were identified as companion cells because they are nucleated,
contain a dense cytoplasm, and are always adjacent to a sieve element. The
labeled cells had the same longitudinal extension as did their sister sieve
elements and always ended next to the sieve plates, which were
characterized by specific staining. PmSUC2 mRNA and PmSUC2 protein were
also detected in P. major roots. The function of PmSUC2 in the different
organs and its role in phloem loading are discussed.
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