THE PLANT CELL, Vol 7, Issue 10 1599-1610, Copyright © 1995 by American Society of Plant Biologists

RESEARCH ARTICLES

CUE1: A Mesophyll Cell-Specific Positive Regulator of Light-Controlled Gene Expression in Arabidopsis

Hm. Li, K. Culligan, R. A. Dixon and J. Chory
Plant Biology Laboratory, Salk Institute, 10010 North Torrey Pines Road, La Jolla, California 92037

Light plays a key role in the development and physiology of plants. One of the most profound effects of light on plant development is the derepression of expression of an array of light-responsive genes, including the genes encoding the chlorophyll a/b binding proteins (CAB) of photosystem II. To understand the mechanism by which light signals nuclear gene expression, we developed a genetic selection to identify mutants with reduced CAB transcription. Here, we describe a new Arabidopsis locus, CUE1 (for CAB underexpressed). Mutations at this locus result in defects in expression of several light-regulated genes, specifically in mesophyll but not in bundle-associated or epidermis cells. Reduced accumulation of CAB and other photosynthesis-related mRNAs in the mesophyll was correlated with defects in chloroplast development in these cells, resulting in a reticulate pattern with veins greener than the interveinal regions of leaves. Moreover, chalcone synthase mRNA, although known to be regulated by both phytochrome and a blue light receptor, accumulated normally in the leaf epidermis. Dark basal levels of CAB expression were unaffected in etiolated cue1 seedlings; however, induction of CAB transcription by pulses of red and blue light was reduced, suggesting that CUE1 acts downstream from both phytochrome and blue light photoreceptors. CUE1 appears to play a role in the primary derepression of mesophyll-specific gene expression in response to light, because cue1 mutants are severely deficient at establishing photoautotrophic growth. Based on this characterization, we propose that CUE1 is a cell-specific positive regulator linking light and intrinsic developmental programs in Arabidopsis leaf mesophyll cells.

This article has been cited by other articles:

				W. Chi, J. Ma, D. Zhang, J. Guo, F. Chen, C. Lu, and L. Zhang The Pentratricopeptide Repeat Protein DELAYED GREENING1 Is Involved in the Regulation of Early Chloroplast Development and Chloroplast Gene Expression in Arabidopsis Plant Physiology, June 1, 2008; 147(2): 573 - 584. [Abstract] [Full Text] [PDF]

				R. F. Baker and D. M. Braun Tie-dyed2 Functions with Tie-dyed1 to Promote Carbohydrate Export from Maize Leaves Plant Physiology, March 1, 2008; 146(3): 1085 - 1097. [Abstract] [Full Text] [PDF]

				C. Andre and C. Benning Arabidopsis Seedlings Deficient in a Plastidic Pyruvate Kinase Are Unable to Utilize Seed Storage Compounds for Germination and Establishment Plant Physiology, December 1, 2007; 145(4): 1670 - 1680. [Abstract] [Full Text] [PDF]

				L. Ding, D. Hofius, M.-R. Hajirezaei, A. R. Fernie, F. Bornke, and U. Sonnewald Functional analysis of the essential bifunctional tobacco enzyme 3-dehydroquinate dehydratase/shikimate dehydrogenase in transgenic tobacco plants J. Exp. Bot., June 1, 2007; 58(8): 2053 - 2067. [Abstract] [Full Text] [PDF]

				C. Andre, J. E. Froehlich, M. R. Moll, and C. Benning A Heteromeric Plastidic Pyruvate Kinase Complex Involved in Seed Oil Biosynthesis in Arabidopsis PLANT CELL, June 1, 2007; 19(6): 2006 - 2022. [Abstract] [Full Text] [PDF]

				I. Heiber, E. Stroher, B. Raatz, I. Busse, U. Kahmann, M. W. Bevan, K.-J. Dietz, and M. Baier The redox imbalanced Mutants of Arabidopsis Differentiate Signaling Pathways for Redox Regulation of Chloroplast Antioxidant Enzymes Plant Physiology, April 1, 2007; 143(4): 1774 - 1788. [Abstract] [Full Text] [PDF]

				E. Lopez-Juez Plastid biogenesis, between light and shadows J. Exp. Bot., January 1, 2007; 58(1): 11 - 26. [Abstract] [Full Text] [PDF]

				R. Gonzalez-Bayon, E. A. Kinsman, V. Quesada, A. Vera, P. Robles, M. R. Ponce, K. A. Pyke, and J. L. Micol Mutations in the RETICULATA gene dramatically alter internal architecture but have little effect on overall organ shape in Arabidopsis leaves J. Exp. Bot., September 1, 2006; 57(12): 3019 - 3031. [Abstract] [Full Text] [PDF]

				A. Hricova, V. Quesada, and J. L. Micol The SCABRA3 Nuclear Gene Encodes the Plastid RpoTp RNA Polymerase, Which Is Required for Chloroplast Biogenesis and Mesophyll Cell Proliferation in Arabidopsis Plant Physiology, July 1, 2006; 141(3): 942 - 956. [Abstract] [Full Text] [PDF]

				Y. Noutoshi, T. Ito, and K. Shinozaki ALBINO AND PALE GREEN 10 Encodes BBMII Isomerase Involved in Histidine Biosynthesis in Arabidopsis thaliana Plant Cell Physiol., July 1, 2005; 46(7): 1165 - 1172. [Abstract] [Full Text] [PDF]

				V. Fey, R. Wagner, K. Brautigam, M. Wirtz, R. Hell, A. Dietzmann, D. Leister, R. Oelmuller, and T. Pfannschmidt Retrograde Plastid Redox Signals in the Expression of Nuclear Genes for Chloroplast Proteins of Arabidopsis thaliana J. Biol. Chem., February 18, 2005; 280(7): 5318 - 5328. [Abstract] [Full Text] [PDF]

				L. M. Voll, E. E. Allaire, G. Fiene, and A. P.M. Weber The Arabidopsis phenylalanine insensitive growth Mutant Exhibits a Deregulated Amino Acid Metabolism Plant Physiology, October 1, 2004; 136(2): 3058 - 3069. [Abstract] [Full Text] [PDF]

				Y. He, R.-H. Tang, Y. Hao, R. D. Stevens, C. W. Cook, S. M. Ahn, L. Jing, Z. Yang, L. Chen, F. Guo, et al. Nitric Oxide Represses the Arabidopsis Floral Transition Science, September 24, 2004; 305(5692): 1968 - 1971. [Abstract] [Full Text] [PDF]

				S. E. Kubis, M. J. Pike, C. J. Everett, L. M. Hill, and S. Rawsthorne The import of phosphoenolpyruvate by plastids from developing embryos of oilseed rape, Brassica napus (L.), and its potential as a substrate for fatty acid synthesis J. Exp. Bot., July 1, 2004; 55(402): 1455 - 1462. [Abstract] [Full Text] [PDF]

				C. S. Chan, H.-P. Peng, and M.-C. Shih Mutations Affecting Light Regulation of Nuclear Genes Encoding Chloroplast Glyceraldehyde-3-Phosphate Dehydrogenase in Arabidopsis Plant Physiology, November 1, 2002; 130(3): 1476 - 1486. [Abstract] [Full Text] [PDF]

				C. Lu, O. A. Koroleva, J. F. Farrar, J. Gallagher, C. J. Pollock, and A. D. Tomos Rubisco Small Subunit, Chlorophyll a/b-Binding Protein and Sucrose:Fructan-6-Fructosyl Transferase Gene Expression and Sugar Status in Single Barley Leaf Cells in Situ. Cell Type Specificity and Induction by Light Plant Physiology, November 1, 2002; 130(3): 1335 - 1348. [Abstract] [Full Text] [PDF]

				M. R. Aluru, H. Bae, D. Wu, and S. R. Rodermel The Arabidopsis immutans Mutation Affects Plastid Differentiation and the Morphogenesis of White and Green Sectors in Variegated Plants Plant Physiology, September 1, 2001; 127(1): 67 - 77. [Abstract] [Full Text] [PDF]

				M. Santiago-Ong, R. M. Green, S. Tingay, J. A. Brusslan, and E. M. Tobin shygrl1 Is a Mutant Affected in Multiple Aspects of Photomorphogenesis Plant Physiology, June 1, 2001; 126(2): 587 - 600. [Abstract] [Full Text] [PDF]

				D. Cao, Y. Lin, and C.-L. Cheng Genetic Interactions between the Chlorate-Resistant Mutant cr 8 8 and the Photomorphogenic Mutants cop1 and hy5 PLANT CELL, February 1, 2000; 12(2): 199 - 210. [Abstract] [Full Text]

				G. P. Copenhaver, K. Nickel, T. Kuromori, M. Benito, S. Kaul, X. Lin, M. Bevan, G. Murphy, B. Harris, L. D. Parnell, et al. Genetic Definition and Sequence Analysis of Arabidopsis Centromeres Science, December 24, 1999; 286(5449): 2468 - 2474. [Abstract] [Full Text]

				H. B. Smith Interorganellar Communication and the Onus of Being Eukaryotic PLANT CELL, September 1, 1999; 11(9): 1605 - 1608. [Full Text]

				S. J. Streatfield, A. Weber, E. A. Kinsman, R. E. Häusler, J. Li, D. Post-Beittenmiller, W. M. Kaiser, K. A. Pyke, U.-I. Flügge, and J. Chory The Phosphoenolpyruvate/Phosphate Translocator Is Required for Phenolic Metabolism, Palisade Cell Development, and Plastid-Dependent Nuclear Gene Expression PLANT CELL, September 1, 1999; 11(9): 1609 - 1622. [Abstract] [Full Text]

				G. Berná, P. Robles, and J. L. Micol A Mutational Analysis of Leaf Morphogenesis in Arabidopsis thaliana Genetics, June 1, 1999; 152(2): 729 - 742. [Abstract] [Full Text]

				T. R. Conley, H.-P. Peng, and M.-C. Shih Mutations Affecting Induction of Glycolytic and Fermentative Genes during Germination and Environmental Stresses in Arabidopsis Plant Physiology, February 1, 1999; 119(2): 599 - 608. [Abstract] [Full Text]

				E. López-Juez, R. Paul Jarvis, A. Takeuchi, A. M. Page, and J. Chory New Arabidopsis cue Mutants Suggest a Close Connection between Plastid- and Phytochrome Regulation of Nuclear Gene Expression Plant Physiology, November 1, 1998; 118(3): 803 - 815. [Abstract] [Full Text]

				P. Jarvis, L. Chen, H. Li, C. A. Peto, C. Fankhauser, and J. Chory An Arabidopsis Mutant Defective in the Plastid General Protein Import Apparatus Science, October 2, 1998; 282(5386): 100 - 103. [Abstract] [Full Text]

				Y. Oono, Q. G. Chen, P. J. Overvoorde, C. Köhler, and A. Theologis age Mutants of Arabidopsis Exhibit Altered Auxin-Regulated Gene Expression PLANT CELL, October 1, 1998; 10(10): 1649 - 1662. [Abstract] [Full Text]

				J. L. Peters, M. Széll, and R. E. Kendrick The Expression of Light-Regulated Genes in the High-Pigment-1 Mutant of Tomato Plant Physiology, July 1, 1998; 117(3): 797 - 807. [Abstract] [Full Text]

				T. Genoud, A. J. Millar, N. Nishizawa, S. A. Kay, E. Schäfer, A. Nagatani, and N.-H. Chua An Arabidopsis Mutant Hypersensitive to Red and Far-Red Light Signals PLANT CELL, June 1, 1998; 10(6): 889 - 904. [Abstract] [Full Text]

				P. Jarvis, P. Dormann, C. A. Peto, J. Lutes, C. Benning, and J. Chory Galactolipid deficiency and abnormal chloroplast development in the Arabidopsis MGD synthase 1 mutant PNAS, July 5, 2000; 97(14): 8175 - 8179. [Abstract] [Full Text] [PDF]