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THE PLANT CELL, Vol 7, Issue 11 1801-1810, Copyright © 1995 by American Society of Plant Biologists
Isolation and Characterization of an Arabidopsis Mutant Deficient in the Thylakoid Lipid Digalactosyl Diacylglycerol
P. Dormann, S. Hoffmann-Benning, I. Balbo and C. Benning
Institut fur Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, 14195 Berlin, Germany
The galactolipids monogalactosyl and digalactosyl diacylglycerol occur in
all higher plants and are the predominant lipid components of chloroplast
membranes. They are thought to be of major importance to chloroplast
morphology and physiology, although direct experimental evidence is still
lacking. The enzymes responsible for final assembly of galactolipids are
associated with the envelope membranes of plastids, and their biochemical
analysis has been notoriously difficult. Therefore, we have chosen a
genetic approach to study the biosynthesis and function of galactolipids in
higher plants. We isolated a mutant of Arabidopsis that is deficient in
digalactosyl diacylglycerol by directly screening a mutagenized M2
population for individuals with altered leaf lipid composition. This mutant
carries a recessive nuclear mutation at a single locus designated dgd1.
Backcrossed mutants show stunted growth, pale green leaf color, reduced
photosynthetic capability, and altered thylakoid membrane ultrastructure.
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