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THE PLANT CELL, Vol 7, Issue 11 1933-1940, Copyright © 1995 by American Society of Plant Biologists
Two NADPH:Protochlorophyllide Oxidoreductases in Barley: Evidence for the Selective Disappearance of PORA during the Light-Induced Greening of Etiolated Seedlings
S. Reinbothe, C. Reinbothe, H. Holtorf and K. Apel
Institute for Plant Sciences, Swiss Federal Institute of Technology Zurich, Universitatsstrasse 2, ETH-Zentrum, CH-8092 Zurich, Switzerland
Chlorophyll synthesis in barley is controlled by two different
light-dependent NADPH:protochlorophyllide oxidoreductases, termed PORA and
PORB. PORA is present abundantly in etioplasts but selectively disappears
soon after the beginning of illumination. This negative light effect is
mediated simultaneously at three different levels. First, the concentration
of porA mRNA declines drastically during illumination of dark-grown
seedlings. Second, the plastids' ability to import the precursor of PORA
(pPORA) is reduced during the transition from etioplasts to chloroplasts.
This effect is due to a rapid decline in the plastidic level of
protochlorophyllide (Pchlide), which is required for the translocation of
the pPORA. Third, PORA becomes selectively destabilized in illuminated
seedlings. When illuminated, PORA-Pchlide-NADPH complexes formed in the
dark photoreduce their Pchlide to Chlide and become simultaneously
susceptible to attack by plastid proteases. The PORA-degrading protease
activity is not detectable in etioplasts but is induced during
illumination. In contrast to PORA, the second Pchlide-reducing enzyme,
PORB, remains operative in both illuminated and green plants. Its
translocation into plastids does not depend on its substrate, Pchlide.
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