THE PLANT CELL, Vol 7, Issue 12 2151-2161, Copyright © 1995 by American Society of Plant Biologists

RESEARCH ARTICLES

Meiotic Recombination Break Points Resolve at High Rates at the 5[prime] End of a Maize Coding Sequence

X. Xu, A. P. Hsia, L. Zhang, B. J. Nikolau and P. S. Schnable
Department of Zoology and Genetics, Iowa State University, Ames, Iowa 50011

Sequence analysis of recombination break points has defined a 377-bp recombination hot spot within the anthocyanin1 (a1) gene. One-fifth of all recombination events that occurred within the 140-kb a1-shrunken2 interval resolved within this 377-bp hot spot. In yeast, meiotic double-strand breaks in chromosomal DNA are thought to initiate recombination and are generally located 5[prime] of coding regions, near transcription promoter sequences. Because the a1 recombination hot spot is located within the 5[prime] transcribed region of the a1 gene, the sites at which recombination events initiate and resolve appear to be different, but both appear to be regulated in relation to transcribed sequences. Although transposon insertions are known to suppress recombination and alter the ratio of crossovers to apparent gene conversions, the Mutator1 transposon insertion in the a1-mum2 allele does not alter the sites at which recombination events resolve.

This article has been cited by other articles:

				J. Li, T.-J. Wen, and P. S. Schnable Role of RAD51 in the Repair of MuDR-Induced Double-Strand Breaks in Maize (Zea mays L.) Genetics, January 1, 2008; 178(1): 57 - 66. [Abstract] [Full Text] [PDF]

				M. D. Yandeau-Nelson, B. J. Nikolau, and P. S. Schnable Effects of trans-acting Genetic Modifiers on Meiotic Recombination Across the a1-sh2 Interval of Maize Genetics, September 1, 2006; 174(1): 101 - 112. [Abstract] [Full Text] [PDF]

				M. D. Yandeau-Nelson, Y. Xia, J. Li, M. G. Neuffer, and P. S. Schnable Unequal Sister Chromatid and Homolog Recombination at a Tandem Duplication of the a1 Locus in Maize Genetics, August 1, 2006; 173(4): 2211 - 2226. [Abstract] [Full Text] [PDF]

				H. Yao and P. S. Schnable Cis-effects on Meiotic Recombination Across Distinct a1-sh2 Intervals in a Common Zea Genetic Background Genetics, August 1, 2005; 170(4): 1929 - 1944. [Abstract] [Full Text] [PDF]

				M. D. Yandeau-Nelson, Q. Zhou, H. Yao, X. Xu, B. J. Nikolau, and P. S. Schnable MuDR Transposase Increases the Frequency of Meiotic Crossovers in the Vicinity of a Mu Insertion in the Maize a1 Gene Genetics, February 1, 2005; 169(2): 917 - 929. [Abstract] [Full Text] [PDF]

				R. M. Clark, E. Linton, J. Messing, and J. F. Doebley Inaugural Article: Pattern of diversity in the genomic region near the maize domestication gene tb1 PNAS, January 20, 2004; 101(3): 700 - 707. [Abstract] [Full Text] [PDF]

				E. S. Balakirev, V. R. Chechetkin, V. V. Lobzin, and F. J. Ayala DNA Polymorphism in the {beta}-Esterase Gene Cluster of Drosophila melanogaster Genetics, June 1, 2003; 164(2): 533 - 544. [Abstract] [Full Text] [PDF]

				L. Huang, S. A. Brooks, W. Li, J. P. Fellers, H. N. Trick, and B. S. Gill Map-Based Cloning of Leaf Rust Resistance Gene Lr21 From the Large and Polyploid Genome of Bread Wheat Genetics, June 1, 2003; 164(2): 655 - 664. [Abstract] [Full Text] [PDF]

				J. D. Faris, J. P. Fellers, S. A. Brooks, and B. S. Gill A Bacterial Artificial Chromosome Contig Spanning the Major Domestication Locus Q in Wheat and Identification of a Candidate Gene Genetics, May 1, 2003; 164(1): 311 - 321. [Abstract] [Full Text] [PDF]

				Y. Vigouroux, J. S. Jaqueth, Y. Matsuoka, O. S. Smith, W. D. Beavis, J. S. C. Smith, and J. Doebley Rate and Pattern of Mutation at Microsatellite Loci in Maize Mol. Biol. Evol., August 1, 2002; 19(8): 1251 - 1260. [Abstract] [Full Text] [PDF]

				H. K. Dooner Extensive Interallelic Polymorphisms Drive Meiotic Recombination into a Crossover Pathway PLANT CELL, May 1, 2002; 14(5): 1173 - 1183. [Abstract] [Full Text] [PDF]

				C. F. Weil Finding the crosswalks on DNA PNAS, April 30, 2002; 99(9): 5763 - 5765. [Full Text] [PDF]

				H. Yao, Q. Zhou, J. Li, H. Smith, M. Yandeau, B. J. Nikolau, and P. S. Schnable From the Cover: Molecular characterization of meiotic recombination across the 140-kb multigenic a1-sh2 interval of maize PNAS, April 30, 2002; 99(9): 6157 - 6162. [Abstract] [Full Text] [PDF]

				D. L. Remington, J. M. Thornsberry, Y. Matsuoka, L. M. Wilson, S. R. Whitt, J. Doebley, S. Kresovich, M. M. Goodman, and E. S. Buckler IV Structure of linkage disequilibrium and phenotypic associations in the maize genome PNAS, September 13, 2001; (2001) 201394398. [Abstract] [Full Text] [PDF]

				Y.-L. Xiao, X. Li, and T. Peterson Ac Insertion Site Affects the Frequency of Transposon-Induced Homologous Recombination at the Maize p1 Locus Genetics, December 1, 2000; 156(4): 2007 - 2017. [Abstract] [Full Text]

				N. Stein, C. Feuillet, T. Wicker, E. Schlagenhauf, and B. Keller Subgenome chromosome walking in wheat: A 450-kb physical contig in Triticum monococcum L. spans the Lr10 resistance locus in hexaploid wheat (Triticum aestivum L.) PNAS, November 8, 2000; (2000) 230361597. [Abstract] [Full Text]

				J. D. Faris, K. M. Haen, and B. S. Gill Saturation Mapping of a Gene-Rich Recombination Hot Spot Region in Wheat Genetics, February 1, 2000; 154(2): 823 - 835. [Abstract] [Full Text]

				W. Hu, M. C. P. Timmermans, and J. Messing Interchromosomal Recombination in Zea mays Genetics, November 1, 1998; 150(3): 1229 - 1237. [Abstract] [Full Text]

				P. Castiglioni, C. Pozzi, M. Heun, V. Terzi, K. J. Muller, W. Rohde, and F. Salamini An AFLP-Based Procedure for the Efficient Mapping of Mutations and DNA Probes in Barley Genetics, August 1, 1998; 149(4): 2039 - 2056. [Abstract] [Full Text] [PDF]

				J. L. Bennetzen, P. SanMiguel, M. Chen, A. Tikhonov, M. Francki, and Z. Avramova Grass genomes PNAS, March 3, 1998; 95(5): 1975 - 1978. [Abstract] [Full Text] [PDF]

				R. A. Martienssen Functional genomics: Probing plant gene function and expression with transposons PNAS, March 3, 1998; 95(5): 2021 - 2026. [Abstract] [Full Text] [PDF]

				J. E. Masson and J. Paszkowski Arabidopsis thaliana mutants altered in homologous recombination PNAS, October 14, 1997; 94(21): 11731 - 11735. [Abstract] [Full Text] [PDF]

				H. Yao, Q. Zhou, J. Li, H. Smith, M. Yandeau, B. J. Nikolau, and P. S. Schnable From the Cover: Molecular characterization of meiotic recombination across the 140-kb multigenic a1-sh2 interval of maize PNAS, April 30, 2002; 99(9): 6157 - 6162. [Abstract] [Full Text] [PDF]

				D. L. Remington, J. M. Thornsberry, Y. Matsuoka, L. M. Wilson, S. R. Whitt, J. Doebley, S. Kresovich, M. M. Goodman, and E. S. Buckler IV Structure of linkage disequilibrium and phenotypic associations in the maize genome PNAS, September 25, 2001; 98(20): 11479 - 11484. [Abstract] [Full Text] [PDF]

				N. Stein, C. Feuillet, T. Wicker, E. Schlagenhauf, and B. Keller Subgenome chromosome walking in wheat: A 450-kb physical contig in Triticum monococcum L. spans the Lr10 resistance locus in hexaploid wheat (Triticum aestivum L.) PNAS, November 21, 2000; 97(24): 13436 - 13441. [Abstract] [Full Text] [PDF]