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THE PLANT CELL, Vol 7, Issue 2 183-193, Copyright © 1995 by American Society of Plant Biologists
Cloning of a Gene Expressed during Appressorium Formation by Colletotrichum gloeosporioides and a Marked Decrease in Virulence by Disruption of This Gene
C. S. Hwang, M. A. Flaishman and P. E. Kolattukudy
Ohio State Biotechnology Center and Biochemistry Program, The Ohio State University, Columbus, Ohio 43210
Appressorium formation in germinating Colletotrichum gloeosporioides is
induced by the surface wax of its host. One of the genes expressed uniquely
in C. gloeosporioides during appressorium formation induced by the host
signal has been designated cap20, and this gene and its cDNA were cloned
and sequenced. Nucleotide sequences of both revealed an open reading frame
that could encode a 183-amino acid polypeptide that did not have
significant homology with any known proteins. Reverse
transcriptase-polymerase chain reaction detected cap20 gene transcripts at
the infection front on the surface and within tomato fruits infected by C.
gloeosporioides. Gene-disrupted mutants incapable of expressing cap20
showed a drastically decreased virulence on avocado and tomato fruits.
These results suggest that cap20 plays a significant role in the infection
of the host.
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