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THE PLANT CELL, Vol 7, Issue 3 259-270, Copyright © 1995 by American Society of Plant Biologists
Impaired Photoassimilate Partitioning Caused by Phloem-Specific Removal of Pyrophosphate Can Be Complemented by a Phloem-Specific Cytosolic Yeast-Derived Invertase in Transgenic Plants
J. Lerchl, P. Geigenberger, M. Stitt and U. Sonnewald
Institut fur Pflanzengenetik und Kulturpflanzenforschung, Corrensstrasse 3, 06466 Gatersleben, Germany
Constitutive expression of the Escherichia coli ppa gene encoding inorganic
pyrophosphatase resulted in sugar accumulation in source leaves and stunted
growth of transgenic tobacco plants. The reason for this phenotype was
hypothesized to be reduced sucrose utilization and loading into the phloem.
To study the role of PPi in phloem cells, a chimeric gene was constructed
using the phloem-specific rolC promoter of Agrobacterium rhizogenes to
drive the expression of the ppa gene. Removal of cytosolic PPi in those
cells resulted in photoassimilate accumulation in source leaves,
chlorophyll loss, and reduced plant growth. From these data, it was
postulated that sucrose hydrolysis via sucrose synthase is essential for
assimilate partitioning. To bypass the PPi-dependent sucrose synthase step,
transgenic plants were produced that express various levels of the yeast
suc2 gene, which encodes cytosolic invertase, in their phloem cells. To
combine the phloem-specific expression of the ppa gene and the suc2 gene,
crosses between invertase- and pyrophosphatase-containing transgenic plants
were performed. Analysis of their offspring revealed that invertase can
complement the phenotypic effects caused by the removal of PPi in phloem
cells.
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