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THE PLANT CELL, Vol 7, Issue 3 271-285, Copyright © 1995 by American Society of Plant Biologists
Specific Expression in Reproductive Tissues and Fate of a Mitochondrial Sterility-Associated Protein in Cytoplasmic Male-Sterile Bean
A. R. Abad, B. J. Mehrtens and S. A. Mackenzie
Department of Agronomy, Purdue University, West Lafayette, Indiana 47907-1150
In common bean, cytoplasmic male sterility has been associated with a
unique sequence found in the mitochondrial genome, designated pvs (for
Phaseolus vulgaris sterility sequence). Within the pvs sequence, two open
reading frames are encoded, ORF98 and ORF239. We have raised rabbit
polyclonal antibodies against Pvs-ORF239 to evaluate the role of this
putative male sterility-associated protein. Histological investigation of
pollen development revealed that in the male-sterile bean line, callose
deposition was abnormal and microspores remained as tetrads as previously
reported. Pvs-ORF239 was found to be localized within the reproductive
tissues of the male-sterile bean line, in contrast to all other cytoplasmic
male sterility systems studied to date. This protein was associated with
mitochondria, the callose layer, and developing primary cell walls during
microsporogenesis. Expression of pvs-orf239 was not detected in fertile
plants containing restorer gene Fr2. These observations, together with
previous reports, suggest that nuclear restorer gene Fr2 interferes with
expression of the pvs region post-transcriptionally.
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