|
THE PLANT CELL, Vol 7, Issue 3 295-307, Copyright © 1995 by American Society of Plant Biologists
Functional Dissection of an Abscisic Acid (ABA)-Inducible Gene Reveals Two Independent ABA-Responsive Complexes Each Containing a G-Box and a Novel cis-Acting Element
Q. Shen and THD. Ho
Plant Biology Program, Department of Biology, Division of Biology and Biomedical Sciences, Washington University, St. Louis, Missouri 63130
To elucidate the mechanism by which abscisic acid (ABA) regulates gene
expression, the promoter of the barley ABA-responsive HVA22 gene has been
analyzed by both loss- and gain-of-function studies. Previous reports
indicate that G-box sequences, which are present in genes responding to a
variety of environmental and physiological cues, are involved in ABA
response. However, our data suggest that G-box sequences are necessary but
not sufficient for ABA response. Instead, an ABA response complex
consisting of a G-box, namely, ABRE3 (GCCACGTACA), and a novel coupling
element, CE1 (TGCCACCGG), is sufficient for high-level ABA induction, and
replacement of either of these sequences abolishes ABA responsiveness. We
suggest that the interaction between G-box sequences, such as ABRE3 in the
HVA22 gene, and CE-type sequences determines the specificity in
ABA-regulated gene expression. Our results also demonstrate that the ABA
response complex is the minimal promoter unit governing high-level ABA
induction; four copies of this 49-bp-long complex linked to a minimal
promoter can confer more than 100-fold ABA-induced gene expression. In
addition to ABA response complex 1, composed of ABRE3 and CE1, the HVA22
promoter contains another ABA response complex. The ABA responsiveness of
this ABA response complex 2 relies on the interaction of a G-box (ABRE2;
CGCACGTGTC) with another yet unidentified coupling element. These two
complexes contribute incrementally to the expression level of HVA22 in
response to ABA.
This article has been cited by other articles:
|
|
|
|
 
W.-J. Guo and T.-H. David Ho
An Abscisic Acid-Induced Protein, HVA22, Inhibits Gibberellin-Mediated Programmed Cell Death in Cereal Aleurone Cells
Plant Physiology,
August 1, 2008;
147(4):
1710 - 1722.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Zhou, H. Zhang, Y. Yang, Z. Zhang, H. Zhang, X. Hu, J. Chen, X.-C. Wang, and R. Huang
Abscisic acid regulates TSRF1-mediated resistance to Ralstonia solanacearum by modifying the expression of GCC box-containing genes in tobacco
J. Exp. Bot.,
February 4, 2008;
(2008)
erm353v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. D. Teaster, C. M. Motes, Y. Tang, W. C. Wiant, M. Q. Cotter, Y.-S. Wang, A. Kilaru, B. J. Venables, K. H. Hasenstein, G. Gonzalez, et al.
N-Acylethanolamine Metabolism Interacts with Abscisic Acid Signaling in Arabidopsis thaliana Seedlings
PLANT CELL,
August 1, 2007;
19(8):
2454 - 2469.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Cao, L. M. Costa, C. Biderre-Petit, B. Kbhaya, N. Dey, P. Perez, D. R. McCarty, J. F. Gutierrez-Marcos, and P. W. Becraft
Abscisic Acid and Stress Signals Induce Viviparous1 Expression in Seed and Vegetative Tissues of Maize
Plant Physiology,
February 1, 2007;
143(2):
720 - 731.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Ogo, R. N. Itai, H. Nakanishi, H. Inoue, T. Kobayashi, M. Suzuki, M. Takahashi, S. Mori, and N. K. Nishizawa
Isolation and characterization of IRO2, a novel iron-regulated bHLH transcription factor in graminaceous plants
J. Exp. Bot.,
August 1, 2006;
57(11):
2867 - 2878.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Tyagi, D. Rajagopal, S. L. Singla-Pareek, M. K. Reddy, and S. K. Sopory
Cloning and Regulation of a Stress-regulated Pennisetum glaucum Vacuolar ATPase c Gene and Characterization of its Promoter that is Expressed in Shoot Hairs and Floral Organs
Plant Cell Physiol.,
August 1, 2005;
46(8):
1411 - 1422.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Xie, Z.-L. Zhang, X. Zou, J. Huang, P. Ruas, D. Thompson, and Q. J. Shen
Annotations and Functional Analyses of the Rice WRKY Gene Superfamily Reveal Positive and Negative Regulators of Abscisic Acid Signaling in Aleurone Cells
Plant Physiology,
January 1, 2005;
137(1):
176 - 189.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Zou, J. R. Seemann, D. Neuman, and Q. J. Shen
A WRKY Gene from Creosote Bush Encodes an Activator of the Abscisic Acid Signaling Pathway
J. Biol. Chem.,
December 31, 2004;
279(53):
55770 - 55779.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Z. Zhang, R. A. Creelman, and J.-K. Zhu
From Laboratory to Field. Using Information from Arabidopsis to Engineer Salt, Cold, and Drought Tolerance in Crops
Plant Physiology,
June 1, 2004;
135(2):
615 - 621.
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z.-L. Zhang, Z. Xie, X. Zou, J. Casaretto, T.-h. D. Ho, and Q. J. Shen
A Rice WRKY Gene Encodes a Transcriptional Repressor of the Gibberellin Signaling Pathway in Aleurone Cells
Plant Physiology,
April 1, 2004;
134(4):
1500 - 1513.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Tsuda, T. Tsuji, S. Hirose, and K.-i. Yamazaki
Three Arabidopsis MBF1 Homologs with Distinct Expression Profiles Play Roles as Transcriptional Co-activators
Plant Cell Physiol.,
February 15, 2004;
45(2):
225 - 231.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ogawa, A. Hanada, Y. Yamauchi, A. Kuwahara, Y. Kamiya, and S. Yamaguchi
Gibberellin Biosynthesis and Response during Arabidopsis Seed Germination
PLANT CELL,
July 1, 2003;
15(7):
1591 - 1604.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Casaretto and T.-h. D. Ho
The Transcription Factors HvABI5 and HvVP1 Are Required for the Abscisic Acid Induction of Gene Expression in Barley Aleurone Cells
PLANT CELL,
January 1, 2003;
15(1):
271 - 284.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Kagaya, T. Hobo, M. Murata, A. Ban, and T. Hattori
Abscisic Acid-Induced Transcription Is Mediated by Phosphorylation of an Abscisic Acid Response Element Binding Factor, TRAB1
PLANT CELL,
December 1, 2002;
14(12):
3177 - 3189.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Niu, T. Helentjaris, and N. J. Bate
Maize ABI4 Binds Coupling Element1 in Abscisic Acid and Sugar Response Genes
PLANT CELL,
October 1, 2002;
14(10):
2565 - 2575.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. A. Iliev, W. Xu, D. H. Polisensky, M.-H. Oh, R. S. Torisky, S. D. Clouse, and J. Braam
Transcriptional and Posttranscriptional Regulation of Arabidopsis TCH4 Expression by Diverse Stimuli. Roles of cis Regions and Brassinosteroids
Plant Physiology,
October 1, 2002;
130(2):
770 - 783.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Xiong, H. Lee, M. Ishitani, Y. Tanaka, B. Stevenson, H. Koiwa, R. A. Bressan, P. M. Hasegawa, and J.-K. Zhu
Repression of stress-responsive genes by FIERY2, a novel transcriptional regulator in Arabidopsis
PNAS,
August 6, 2002;
99(16):
10899 - 10904.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. YOKOTA, S. KAWASAKI, M. IWANO, C. NAKAMURA, C. MIYAKE, and K. AKASHI
Citrulline and DRIP-1 Protein (ArgE Homologue) in Drought Tolerance of Wild Watermelon
Ann. Bot.,
June 15, 2002;
89(7):
825 - 832.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Hattori, M. Totsuka, T. Hobo, Y. Kagaya, and A. Yamamoto-Toyoda
Experimentally Determined Sequence Requirement of ACGT-Containing Abscisic Acid Response Element
Plant Cell Physiol.,
January 1, 2002;
43(1):
136 - 140.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Xiong, M. Ishitani, H. Lee, and J.-K. Zhu
The Arabidopsis LOS5/ABA3 Locus Encodes a Molybdenum Cofactor Sulfurase and Modulates Cold Stress- and Osmotic Stress-Responsive Gene Expression
PLANT CELL,
September 1, 2001;
13(9):
2063 - 2083.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Xiong, B.-h. Lee, M. Ishitani, H. Lee, C. Zhang, and J.-K. Zhu
FIERY1 encoding an inositol polyphosphate 1-phosphatase is a negative regulator of abscisic acid and stress signaling in Arabidopsis
Genes & Dev.,
August 1, 2001;
15(15):
1971 - 1984.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Tang, A. Sadka, D. T. Morishige, and J. E. Mullet
Homeodomain Leucine Zipper Proteins Bind to the Phosphate Response Domain of the Soybean VspB Tripartite Promoter
Plant Physiology,
February 1, 2001;
125(2):
797 - 809.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Y. Uno, T. Furihata, H. Abe, R. Yoshida, K. Shinozaki, and K. Yamaguchi-Shinozaki
Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions
PNAS,
September 22, 2000;
(2000)
190309197.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. Hobo, Y. Kowyama, and T. Hattori
A bZIP factor, TRAB1, interacts with VP1 and mediates abscisic acid-induced transcription
PNAS,
December 21, 1999;
96(26):
15348 - 15353.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Gomez-Cadenas, S. D. Verhey, L. D. Holappa, Q. Shen, T.-H. D. Ho, and M. K. Walker-Simmons
An abscisic acid-induced protein kinase, PKABA1, mediates abscisic acid-suppressed gene expression in barley aleurone layers
PNAS,
February 16, 1999;
96(4):
1767 - 1772.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. B. Singh
Transcriptional Regulation in Plants: The Importance of Combinatorial Control
Plant Physiology,
December 1, 1998;
118(4):
1111 - 1120.
[Full Text]
|
|
|
|
|
|
|
J. Su, Q. Shen, T.-H. David Ho, and R. Wu
Dehydration-Stress-Regulated Transgene Expression in Stably Transformed Rice Plants
Plant Physiology,
July 1, 1998;
117(3):
913 - 922.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
F. U. Hoeren, R. Dolferus, Y. Wu, W. J. Peacock, and E. S. Dennis
Evidence for a Role for AtMYB2 in the Induction of the Arabidopsis Alcohol Dehydrogenase Gene (ADH1) Low Oxygen
Genetics,
June 1, 1998;
149(2):
479 - 490.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. B. Treviño and M. A. O' Connell
Three Drought-Responsive Members of the Nonspecific Lipid-Transfer Protein Gene Family in Lycopersicon pennellii Show Different Developmental Patterns of Expression
Plant Physiology,
April 1, 1998;
116(4):
1461 - 1468.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. Sheen
Ca2+-Dependent Protein Kinases and Stress Signal Transduction in Plants
Science,
December 13, 1996;
274(5294):
1900 - 1902.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Y. Uno, T. Furihata, H. Abe, R. Yoshida, K. Shinozaki, and K. Yamaguchi-Shinozaki
Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions
PNAS,
October 10, 2000;
97(21):
11632 - 11637.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
