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THE PLANT CELL, Vol 7, Issue 5 611-621, Copyright © 1995 by American Society of Plant Biologists
Post-Transcriptional Regulation of Nitrate Reductase by Light Is Abolished by an N-Terminal Deletion
L. Nussaume, M. Vincentz, C. Meyer, J. P. Boutin and M. Caboche
Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, Centre de Versailles, Route de Saint-Cyr, 78026 Versailles Cedex, France
Higher plant nitrate reductases (NRs) carry an N-terminal domain whose
sequence is not conserved in NRs from other organisms. A gene composed of a
full-length tobacco NR cDNA with an internal deletion of 168 bp in the
5[prime] end fused to the cauliflower mosaic virus 35S promoter and
appropriate termination signals was constructed and designated as
[delta]NR. An NR-deficient mutant of Nicotiana plumbaginifolia was
transformed with this [delta]NR gene. In transgenic plants expressing this
construct, NR activity was restored and normal growth resulted. Apart from
a higher thermosensitivity, no appreciable modification of the kinetic
parameters of the enzyme was detectable. The post-transcriptional
regulation of NR by light was abolished in [delta]NR transformants.
Consequently, deregulated production of glutamine and asparagine was
detected in [delta]NR transformants. The absence of in vitro [delta]NR
activity modulation by ATP suggests the impairment of [delta]NR
phosphorylation and thereby suppression of [delta]NR post-translational
regulation. These data imply that post-transcriptional control of NR
expression is important for the flow of the nitrate assimilatory pathway.
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