|
THE PLANT CELL, Vol 7, Issue 5 639-647, Copyright © 1995 by American Society of Plant Biologists
Transient Activation and Tyrosine Phosphorylation of a Protein Kinase in Tobacco Cells Treated with a Fungal Elicitor
K. Suzuki and H. Shinshi
Plant Molecular Biology Laboratory, Molecular Biology Department, National Institute of Bioscience and Human Technology, AIST, MITI, Higashi 1-1, Tsukuba 305, Japan
Suspension-cultured tobacco cells respond to fungal elicitor by activating
the transcription of so-called defense genes. This response has been shown
to be blocked by staurosporine, an inhibitor of protein kinases, and by
Gd3+, which blocks Ca2+ channels. We report here that treating tobacco
cells with the elicitor triggers the rapid and transient activation of a
47-kD protein kinase that phosphorylates serine and/or threonine residues
of the myelin basic protein (MBP). Staurosporine and Gd3+ inhibited the
elicitor-induced activation of the 47-kD MBP kinase, and staurosporine
inhibited the activity of the MBP kinase itself. In the presence of either
cycloheximide or calyculin A, the elicitor induced sustained activation of
the MBP kinase. Immunoblot and immunoprecipitation analysis using a
phosphotyrosine-specific antibody showed that tyrosine phosphorylation of
the 47-kD MBP kinase was induced in tobacco cells that had been treated
with the elicitor. The results suggest that the 47-kD MBP kinase is a
component of the pathway for transduction of the elicitor signal in tobacco
cells and that the activity of the MBP kinase is regulated by the
post-translational phosphorylation of tyrosine residues.
This article has been cited by other articles:
|
|
|
|
 
A. Heese, D. R. Hann, S. Gimenez-Ibanez, A. M. E. Jones, K. He, J. Li, J. I. Schroeder, S. C. Peck, and J. P. Rathjen
The receptor-like kinase SERK3/BAK1 is a central regulator of innate immunity in plants
PNAS,
July 17, 2007;
104(29):
12217 - 12222.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Kadota, T. Watanabe, S. Fujii, Y. Maeda, R. Ohno, K. Higashi, T. Sano, S. Muto, S. Hasezawa, and K. Kuchitsu
Cell Cycle Dependence of Elicitor-induced Signal Transduction in Tobacco BY-2 Cells
Plant Cell Physiol.,
January 15, 2005;
46(1):
156 - 165.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. H. Cheong, B. C. Moon, J. K. Kim, C. Y. Kim, M. C. Kim, I. H. Kim, C. Y. Park, J. C. Kim, B. O. Park, S. C. Koo, et al.
BWMK1, a Rice Mitogen-Activated Protein Kinase, Locates in the Nucleus and Mediates Pathogenesis-Related Gene Expression by Activation of a Transcription Factor
Plant Physiology,
August 1, 2003;
132(4):
1961 - 1972.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Rudrabhatla and R. Rajasekharan
Mutational Analysis of Stress-responsive Peanut Dual Specificity Protein Kinase. IDENTIFICATION OF TYROSINE RESIDUES INVOLVED IN REGULATION OF PROTEIN KINASE ACTIVITY
J. Biol. Chem.,
May 2, 2003;
278(19):
17328 - 17335.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Xiong and Y. Yang
Disease Resistance and Abiotic Stress Tolerance in Rice Are Inversely Modulated by an Abscisic Acid-Inducible Mitogen-Activated Protein Kinase
PLANT CELL,
March 1, 2003;
15(3):
745 - 759.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Rudrabhatla and R. Rajasekharan
Developmentally Regulated Dual-Specificity Kinase from Peanut That Is Induced by Abiotic Stresses
Plant Physiology,
September 1, 2002;
130(1):
380 - 390.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Bauer, L. Gomez-Gomez, T. Boller, and G. Felix
Sensitivity of Different Ecotypes and Mutants of Arabidopsis thaliana toward the Bacterial Elicitor Flagellin Correlates with the Presence of Receptor-binding Sites
J. Biol. Chem.,
November 30, 2001;
276(49):
45669 - 45676.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Zhang and Y. Liu
Activation of Salicylic Acid-Induced Protein Kinase, a Mitogen-Activated Protein Kinase, Induces Multiple Defense Responses in Tobacco
PLANT CELL,
August 1, 2001;
13(8):
1877 - 1889.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Osawa and H. Matsumoto
Possible Involvement of Protein Phosphorylation in Aluminum-Responsive Malate Efflux from Wheat Root Apex
Plant Physiology,
May 1, 2001;
126(1):
411 - 420.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
R. Ulm, E. Revenkova, G.-P. di Sansebastiano, N. Bechtold, and J. Paszkowski
Mitogen-activated protein kinase phosphatase is required for genotoxic stress relief in Arabidopsis
Genes & Dev.,
March 15, 2001;
15(6):
699 - 709.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
K. Sugimoto, S. Takeda, and H. Hirochika
MYB-Related Transcription Factor NtMYB2 Induced by Wounding and Elicitors is a Regulator of the Tobacco Retrotransposon Tto1 and Defense-Related Genes
PLANT CELL,
December 1, 2000;
12(12):
2511 - 2528.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
G. Felix, M. Regenass, and T. Boller
Sensing of Osmotic Pressure Changes in Tomato Cells
Plant Physiology,
November 1, 2000;
124(3):
1169 - 1180.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Y. S. Kim, J. H. Lee, G. M. Yoon, H. S. Cho, S.-W. Park, M. C. Suh, D. Choi, H. J. Ha, J. R. Liu, and H.-S. Pai
CHRK1, a Chitinase-Related Receptor-Like Kinase in Tobacco
Plant Physiology,
July 1, 2000;
123(3):
905 - 916.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Y. Huang, H. Li, R. Gupta, P. C. Morris, S. Luan, and J. J. Kieber
ATMPK4, an Arabidopsis Homolog of Mitogen-Activated Protein Kinase, Is Activated in Vitro by AtMEK1 through Threonine Phosphorylation
Plant Physiology,
April 1, 2000;
122(4):
1301 - 1310.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. S. Nuhse, S. C. Peck, H. Hirt, and T. Boller
Microbial Elicitors Induce Activation and Dual Phosphorylation of the Arabidopsis thaliana MAPK 6
J. Biol. Chem.,
March 10, 2000;
275(11):
7521 - 7526.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Olivari, C. Albumi, M. C. Pugliarello, and M. I. De Michelis
Phenylarsine Oxide Inhibits the Fusicoccin-Induced Activation of Plasma Membrane H+-ATPase
Plant Physiology,
February 1, 2000;
122(2):
463 - 470.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. Mikolajczyk, O. S. Awotunde, G. Muszynska, D. F. Klessig, and G. Dobrowolska
Osmotic Stress Induces Rapid Activation of a Salicylic Acid-Induced Protein Kinase and a Homolog of Protein Kinase ASK1 in Tobacco Cells
PLANT CELL,
January 1, 2000;
12(1):
165 - 178.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Tripathy, B. J. Venables, and K. D. Chapman
N-Acylethanolamines in Signal Transduction of Elicitor Perception. Attenuation of Alkalinization Response and Activation of Defense Gene Expression
Plant Physiology,
December 1, 1999;
121(4):
1299 - 1308.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
V. K. Rajasekhar, C. Lamb, and R. A. Dixon
Early Events in the Signal Pathway for the Oxidative Burst in Soybean Cells Exposed to Avirulent Pseudomonas syringae pv glycinea
Plant Physiology,
August 1, 1999;
120(4):
1137 - 1146.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
K. Suzuki, A. Yano, and H. Shinshi
Slow and Prolonged Activation of the p47 Protein Kinase during Hypersensitive Cell Death in a Culture of Tobacco Cells
Plant Physiology,
April 1, 1999;
119(4):
1465 - 1472.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. Romeis, P. Piedras, S. Zhang, D. F. Klessig, H. Hirt, and J. D. G. Jones
Rapid Avr 9- and Cf-9 –Dependent Activation of MAP Kinases in Tobacco Cell Cultures and Leaves: Convergence of Resistance Gene, Elicitor, Wound, and Salicylate Responses
PLANT CELL,
February 1, 1999;
11(2):
273 - 288.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. Meindl, T. Boller, and G. Felix
The Plant Wound Hormone Systemin Binds with the N-Terminal Part to Its Receptor but Needs the C-Terminal Part to Activate It
PLANT CELL,
September 1, 1998;
10(9):
1561 - 1570.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
I. Islas-Flores, C. Oropeza, and S.M. Teresa Hernández-Sotomayor
Protein Phosphorylation during Coconut Zygotic Embryo Development
Plant Physiology,
September 1, 1998;
118(1):
257 - 263.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. Zhang and D. F. Klessig
Resistance gene N-mediated de novo synthesis and activation of a tobacco mitogen-activated protein kinase by tobacco mosaic virus infection
PNAS,
June 23, 1998;
95(13):
7433 - 7438.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Zhang and D. F. Klessig
The tobacco wounding-activated mitogen-activated protein kinase is encoded by SIPK
PNAS,
June 9, 1998;
95(12):
7225 - 7230.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Q. Xu, H.-H. Fu, R. Gupta, and S. Luan
Molecular Characterization of a Tyrosine-Specific Protein Phosphatase Encoded by a Stress-Responsive Gene in Arabidopsis
PLANT CELL,
May 1, 1998;
10(5):
849 - 858.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Zhang, H. Du, and D. F. Klessig
Activation of the Tobacco SIP Kinase by Both a Cell Wall–Derived Carbohydrate Elicitor and Purified Proteinaceous Elicitins from Phytophthora spp
PLANT CELL,
March 1, 1998;
10(3):
435 - 450.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. D. Chapman, S. Tripathy, B. Venables, and A. D. Desouza
N-Acylethanolamines: Formation and Molecular Composition of a New Class of Plant Lipids
Plant Physiology,
March 1, 1998;
116(3):
1163 - 1168.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
K. A. Johnson, M. L. Sistrunk, D. H. Polisensky, and J. Braam
Arabidopsis thaliana Responses to Mechanical Stimulation Do Not Require ETR1 or EIN2
Plant Physiology,
February 1, 1998;
116(2):
643 - 649.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O Calderini, L Bogre, O Vicente, P Binarova, E Heberle-Bors, and C Wilson
A cell cycle regulated MAP kinase with a possible role in cytokinesis in tobacco cells
J. Cell Sci.,
January 10, 1998;
111(20):
3091 - 3100.
[Abstract]
[PDF]
|
|
|
|
|
|
|
Y Yang, J Shah, and D F Klessig
Signal perception and transduction in plant defense responses.
Genes & Dev.,
July 1, 1997;
11(13):
1621 - 1639.
[PDF]
|
|
|
|
|
|
|
S. Zimmermann, T. Nurnberger, J.-M. Frachisse, W. Wirtz, J. Guern, R. Hedrich, and D. Scheel
Receptor-mediated activation of a plant Ca2+-permeable ion channel involved in pathogen defense
PNAS,
March 18, 1997;
94(6):
2751 - 2755.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Chandra, G. B. Martin, and P. S. Low
The Pto kinase mediates a signaling pathway leading to the oxidative burst in tomato
PNAS,
November 12, 1996;
93(23):
13393 - 13397.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. M. Logan, N. Cathala, C. Grignon, and J.-C. Davidian
Cloning of a cDNA Encoded by a Member of the Arabidopsis thaliana ATP Sulfurylase Multigene Family
J. Biol. Chem.,
May 24, 1996;
271(21):
12227 - 12233.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Seo, M. Okamoto, H. Seto, K. Ishizuka, H. Sano, and Y. Ohashi
Tobacco MAP Kinase: A Possible Mediator in Wound Signal Transduction Pathways
Science,
December 22, 1995;
270(5244):
1988 - 1992.
[Abstract]
[PDF]
|
|
|
|
|
|
|
F. Cardinale, C. Jonak, W. Ligterink, K. Niehaus, T. Boller, and H. Hirt
Differential Activation of Four Specific MAPK Pathways by Distinct Elicitors
J. Biol. Chem.,
November 17, 2000;
275(47):
36734 - 36740.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
