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THE PLANT CELL, Vol 7, Issue 5 649-658, Copyright © 1995 by American Society of Plant Biologists
Inactivation of a Synechocystis sp Strain PCC 6803 Gene with Homology to Conserved Chloroplast Open Reading Frame 184 Increases the Photosystem II-to-Photosystem I Ratio
A. Wilde, H. Hartel, T. Hubschmann, P. Hoffmann, S. V. Shestakov and T. Borner
Institut fur Biologie, Humboldt-Universitat zu Berlin, Invalidenstrasse 43, D-10115 Berlin, Germany
A gene of the unicellular cyanobacterium Synechocystis sp strain PCC 6803
that is homologous to the conserved chloroplast open reading frame orf184
has been cloned and sequenced. The nucleotide sequence of the gene predicts
a protein of 184 amino acids with a calculated molecular mass of 21.5 kD
and two membrane-spanning regions. Amino acid sequence analysis showed 46
to 37% homology of the cyanobacterial orf184 with tobacco orf184, rice
orf185, liverwort orf184, and Euglena gracilis orf206 sequences. Two
orf184-specific mutants of Synechocystis sp PCC 6803 were constructed by
insertion mutagenesis. Cells of mutants showed growth characteristics
similar to those of the wild type. Their pigment composition was distinctly
different from the wild type, as indicated by an increase in the
phycocyanin-to-chlorophyll ratio. In addition, mutants also had a two- to
threefold increase in photosynthetic electron transfer rates as well as in
photosystem II-to-photosystem I ratio [mdash] a phenomenon hitherto not
reported for mutants with altered photosynthetic characteristics. The
observed alterations in the orf184-specific mutants provide strong evidence
for a functional role of the orf184 gene product in photosynthetic
processes.
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