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THE PLANT CELL, Vol 7, Issue 6 759-771, Copyright © 1995 by American Society of Plant Biologists
cycMs3, a Novel B-Type Alfalfa Cyclin Gene, Is Induced in the G0-to-G1 Transition of the Cell Cycle
I. Meskiene, L. Bogre, M. Dahl, M. Pirck, DTC. Ha, I. Swoboda, E. Heberle-Bors, G. Ammerer and H. Hirt
Vienna Biocenter, Institute of Microbiology and Genetics, Dr. Bohrgasse 9, A-1030 Vienna, Austria
Cyclins are key regulators of the cell cycle in all eukaryotes. We have
previously isolated two B-type cyclin genes, cycMs1 and cycMs2, from
alfalfa that are primarily expressed during the G2-to-M phase transition
and are most likely mitotic cyclin genes. Here, we report the isolation of
a novel alfalfa cyclin gene, termed cycMs3 (for cyclin Medicago sativa), by
selecting for mating type [alpha]-pheromone-induced cell cycle arrest
suppression in yeast. The central region of the predicted amino acid
sequence of the cycMs3 gene is most similar to the cyclin box of yeast
B-type and mammalian A- and B-type cyclins. In situ hybridization showed
that cycMs3 mRNA can be detected only in proliferating cells and not in
differentiated alfalfa cells. When differentiated G0-arrested cells were
induced to reenter the cell cycle in the G1 phase and resume cell division
by treatment with plant hormones, cycMs3 transcript levels increased long
before the onset of DNA synthesis. In contrast, histone H3-1 mRNA and
cycMs2 transcripts were not observed before DNA replication and mitosis,
respectively. In addition, cycMs3 mRNA was found in all stages of the cell
cycle in synchronously dividing cells, whereas the cycMs2 and histone H3-1
genes showed a G2-to-M phase- or S phase-specific transcription pattern,
respectively. These data suggest that the role of cyclin CycMs3 differs
from that of CycMs1 and CycMs2. We propose that CycMs3 helps control
reentry of quiescent G0-arrested cells into the G1 phase of the cell cycle.
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