|
THE PLANT CELL, Vol 7, Issue 8 1295-1305, Copyright © 1995 by American Society of Plant Biologists
The Initiation Codon Determines the Efficiency but Not the Site of Translation Initiation in Chlamydomonas Chloroplasts
X. Chen, K. L. Kindle and D. B. Stern
Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, New York 14853
To study translation initiation in Chlamydomonas chloroplasts, we mutated
the initiation codon AUG to AUU, ACG, ACC, ACU, and UUC in the chloroplast
petA gene, which encodes cytochrome f of the cytochrome b6/f complex.
Cytochrome f accumulated to detectable levels in all mutant strains except
the one with a UUC codon, but only the mutant with an AUU codon grew well
at 24[deg]C under conditions that require photosynthesis. Because no
cytochrome f was detectable in the UUC mutant and because each mutant that
accumulated cytochrome f did so at a different level, we concluded that any
residual translation probably initiates at the mutant codon. As a further
demonstration that alternative initiation sites are not used in vivo, we
introduced in-frame UAA stop codons immediately downstream or upstream or
in place of the initiation codon. Stop codons at or downstream of the
initiation codon prevented accumulation of cytochrome f, whereas the one
immediately upstream of the initiation codon had no effect on the
accumulation of cytochrome f. These results suggest that an AUG codon is
not required to specify the site of translation initiation in chloroplasts
but that the efficiency of translation initiation depends on the identity
of the initiation codon.
This article has been cited by other articles:
|
|
|
|
 
P. Yang, C. Yang, and W. S. Sale
Flagellar Radial Spoke Protein 2 Is a Calmodulin Binding Protein Required for Motility in Chlamydomonas reinhardtii
Eukaryot. Cell,
February 1, 2004;
3(1):
72 - 81.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Zandueta-Criado and R. Bock
Surprising features of plastid ndhD transcripts: addition of non-encoded nucleotides and polysome association of mRNAs with an unedited start codon
Nucleic Acids Res.,
January 26, 2004;
32(2):
542 - 550.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Baroli, A. D. Do, T. Yamane, and K. K. Niyogi
Zeaxanthin Accumulation in the Absence of a Functional Xanthophyll Cycle Protects Chlamydomonas reinhardtii from Photooxidative Stress
PLANT CELL,
April 1, 2003;
15(4):
992 - 1008.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. W. Dreyfuss, P. P. Hamel, S. S. Nakamoto, and S. Merchant
Functional Analysis of a Divergent System II Protein, Ccs1, Involved in c-Type Cytochrome Biogenesis
J. Biol. Chem.,
January 17, 2003;
278(4):
2604 - 2613.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Esposito, A. J. Hicks, and D. B. Stern
A Role for Initiation Codon Context in Chloroplast Translation
PLANT CELL,
October 1, 2001;
13(10):
2373 - 2384.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. W. Odom, S. P. Holloway, N. N. Deshpande, J. Lee, and D. L. Herrin
Mobile Self-Splicing Group I Introns from the psbA Gene of Chlamydomonas reinhardtii: Highly Efficient Homing of an Exogenous Intron Containing Its Own Promoter
Mol. Cell. Biol.,
May 15, 2001;
21(10):
3472 - 3481.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
F. Ossenbühl and J. Nickelsen
cis- and trans-Acting Determinants for Translation of psbD mRNA in Chlamydomonas reinhardtii
Mol. Cell. Biol.,
November 1, 2000;
20(21):
8134 - 8142.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
W. Majeran, F.-A. Wollman, and O. Vallon
Evidence for a Role of ClpP in the Degradation of the Chloroplast Cytochrome b6f Complex
PLANT CELL,
January 1, 2000;
12(1):
137 - 150.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. J. McCormac and A. Barkan
A Nuclear Gene in Maize Required for the Translation of the Chloroplast atpB/E mRNA
PLANT CELL,
September 1, 1999;
11(9):
1709 - 1716.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. Nickelsen, M. Fleischmann, E. Boudreau, M. Rahire, and J.-D. Rochaix
Identification of cis-Acting RNA Leader Elements Required for Chloroplast psbD Gene Expression in Chlamydomonas
PLANT CELL,
May 1, 1999;
11(5):
957 - 970.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Z. Xie, D. Culler, B. W. Dreyfuss, R. Kuras, F.-A. Wollman, J. Girard-Bascou, and S. Merchant
Genetic Analysis of Chloroplast c -Type Cytochrome Assembly in Chlamydomonas reinhardtii: One Chloroplast Locus and at Least Four Nuclear Loci Are Required for Heme Attachment
Genetics,
February 1, 1998;
148(2):
681 - 692.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
