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THE PLANT CELL, Vol 7, Issue 9 1387-1394, Copyright © 1995 by American Society of Plant Biologists

RESEARCH ARTICLES

High-Level Tuber Expression and Sucrose Inducibility of a Potato Sus4 Sucrose Synthase Gene Require 5[prime] and 3[prime] Flanking Sequences and the Leader Intron

H. Fu, S. Y. Kim and W. D. Park
Department of Biochemistry and Biophysics, Texas A & M University, College Station, Texas 77843-2128

The 3.6 kb of 5[prime] flanking sequence, leader intron, and 0.7 kb of 3[prime] sequence from the potato sucrose synthase gene Sus4-16 are sufficient to direct high-level expression in developing tubers, in basal tissues of axillary buds and shoots, and in meristems and caps of roots, and to confer sucrose inducibility in leaves. By examining a series of deletion and substitution constructs in transgenic potato plants, we found that this pattern of expression requires 5[prime] flanking sequences both upstream and downstream of position -1500 and that sequences between positions -1500 and -267 are essential for sucrose induction. Replacement of the native 3[prime] sequence with the nopaline synthase 3[prime] sequence resulted in the loss of sucrose inducibility and of expression in basal tissues of axillary buds. A general decrease in expression in other tissues was also observed. Removal of the 1612-bp leader intron also had a dramatic effect on both the pattern and level of expression.


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Copyright © 1995 by the American Society of Plant Biologists