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THE PLANT CELL, Vol 7, Issue 9 1395-1403, Copyright © 1995 by American Society of Plant Biologists
A Potato Sus3 Sucrose Synthase Gene Contains a Context-Dependent 3[prime] Element and a Leader Intron with Both Positive and Negative Tissue-Specific Effects
H. Fu, S. Y. Kim and W. D. Park
Department of Biochemistry and Biophysics, Texas A & M University, College Station, Texas 77843-2128
To examine which sequences are involved in regulating the potato sucrose
synthase gene Sus3-65, we examined a series of deletion and substitution
constructs in transgenic potato and tobacco plants. In a construct
containing 3.9 kb of 5[prime] flanking region, substitution of the native
3[prime] sequence with the nopaline synthase 3[prime] sequence and deletion
of the leader intron did not significantly affect expression in vegetative
tissues. However, in a construct containing only 320 bp of 5[prime]
flanking region, these changes had marked effects. Replacing the native
3[prime] sequences with nopaline synthase 3[prime] sequences caused a six-
to 20-fold increase in expression in vascular tissue, and removing the
leader intron almost completely abolished expression in potato plants.
Surprisingly, removal of the leader intron from either the full-length
construct or a construct containing only 320 bp of 5[prime] flanking
sequence reduced expression in vascular tissue of tobacco anthers at later
stages of development but increased expression in pollen by more than
100-fold.
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