|
THE PLANT CELL, Vol 7, Issue 9 1445-1457, Copyright © 1995 by American Society of Plant Biologists
Identification of a Sequence-Specific DNA Binding Factor Required for Transcription of the Barley Chloroplast Blue Light-Responsive psbD-psbC Promoter
M. Kim and J. E. Mullet
Department of Biochemistry and Biophysics, Texas A & M University, College Station, Texas 77843
The plastid gene psbD encodes the photosystem II reaction center
chlorophyll protein D2. psbD is located in a complex operon that includes
psbC, psbK, psbl, orf62, and trnG. The operon is transcribed from at least
three different promoters. One of the psbD promoters is differentially
activated when plants are exposed to blue light. In this study, the psbD
blue light-responsive promoter was accurately transcribed in vitro in
high-salt extracts of barley plastids. Transcription required supercoiled
templates and was inhibited by tagetitoxin, an inhibitor of plastid
transcription. Escherichia coli RNA polymerase did not recognize the psbD
light-responsive promoter with the same specificity as plastid RNA
polymerase. Deletion analyses demonstrated that sequences between -39 and
-68, upstream of the transcription initiation site, were required for
transcription of the psbD blue light-responsive promoter. This DNA region
is highly conserved among plant species and contains multiple AAG
sequences. Gel shift assays and DNase I footprinting experiments
demonstrated that the AAG-rich DNA sequence interacts with a
sequence-specific DNA binding factor termed AGF. Point mutations in the AAG
cis element decreased binding of AGF and inhibited transcription from the
psbD light-responsive promoter. We concluded that AGF is an essential
factor required for transcription of the psbD light-responsive promoter.
This article has been cited by other articles:
|
|
|
|
 
E. Demarsy, F. Courtois, J. Azevedo, L. Buhot, and S. Lerbs-Mache
Building Up of the Plastid Transcriptional Machinery during Germination and Early Plant Development
Plant Physiology,
November 1, 2006;
142(3):
993 - 1003.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Nagashima, M. Hanaoka, T. Shikanai, M. Fujiwara, K. Kanamaru, H. Takahashi, and K. Tanaka
The Multiple-Stress Responsive Plastid Sigma Factor, SIG5, Directs Activation of the psbD Blue Light-Responsive Promoter (BLRP) in Arabidopsis thaliana
Plant Cell Physiol.,
April 15, 2004;
45(4):
357 - 368.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Tsunoyama, Y. Ishizaki, K. Morikawa, M. Kobori, Y. Nakahira, G. Takeba, Y. Toyoshima, and T. Shiina
Blue light-induced transcription of plastid-encoded psbD gene is mediated by a nuclear-encoded transcription initiation factor, AtSig5
PNAS,
March 2, 2004;
101(9):
3304 - 3309.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Y. Suzuki, P. Sriraman, Z. Svab, and P. Maliga
Unique Architecture of the Plastid Ribosomal RNA Operon Promoter Recognized by the Multisubunit RNA Polymerase in Tobacco and Other Higher Plants
PLANT CELL,
January 1, 2003;
15(1):
195 - 205.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. E. Thum, M. Kim, D. A. Christopher, and J. E. Mullet
Cryptochrome 1, Cryptochrome 2, and Phytochrome A Co-Activate the Chloroplast psbD Blue Light-Responsive Promoter
PLANT CELL,
December 1, 2001;
13(12):
2747 - 2760.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Sato and N. Ohta
DNA-binding specificity and dimerization of the DNA-binding domain of the PEND protein in the chloroplast envelope membrane
Nucleic Acids Res.,
June 1, 2001;
29(11):
2244 - 2250.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Baba, T. Nakano, K. Yamagishi, and S. Yoshida
Involvement of a Nuclear-Encoded Basic Helix-Loop-Helix Protein in Transcription of the Light-Responsive Promoter of psbD
Plant Physiology,
February 1, 2001;
125(2):
595 - 603.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C.-C. Chang, J. Sheen, M. Bligny, Y. Niwa, S. Lerbs-Mache, and D. B. Stern
Functional Analysis of Two Maize cDNAs Encoding T7-like RNA Polymerases
PLANT CELL,
May 1, 1999;
11(5):
911 - 926.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. Tan and R. F. Troxler
Characterization of two chloroplast RNA polymerase sigma factors from Zea mays: Photoregulation and differential expression
PNAS,
April 27, 1999;
96(9):
5316 - 5321.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kim, K. E. Thum, D. T. Morishige, and J. E. Mullet
Detailed Architecture of the Barley Chloroplast psbD-psbC Blue Light-responsive Promoter
J. Biol. Chem.,
February 19, 1999;
274(8):
4684 - 4692.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kim, D. A. Christopher, and J. E. Mullet
ADP-Dependent Phosphorylation Regulates Association of a DNA-Binding Complex with the Barley Chloroplast psbD Blue-Light-Responsive Promoter
Plant Physiology,
February 1, 1999;
119(2):
663 - 670.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Y. Nakahira, K. Baba, A. Yoneda, T. Shiina, and Y. Toyoshima
Circadian-Regulated Transcription of the psbD Light-Responsive Promoter in Wheat Chloroplasts
Plant Physiology,
November 1, 1998;
118(3):
1079 - 1088.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. Shiina, L. Allison, and P. Maliga
rbcL Transcript Levels in Tobacco Plastids Are Independent of Light: Reduced Dark Transcription Rate Is Compensated by Increased mRNA Stability
PLANT CELL,
October 1, 1998;
10(10):
1713 - 1722.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. Hong and R. J. Spreitzer
Nuclear-Gene Mutations Suppress a Defect in the Expression of the Chloroplast-Encoded Large Subunit of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase
Plant Physiology,
April 1, 1998;
116(4):
1387 - 1392.
[Abstract]
[Full Text]
|
|
|
|
|
