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THE PLANT CELL, Vol 8, Issue 1 43-53, Copyright © 1996 by American Society of Plant Biologists
A Soluble Protein Is Imported into Euglena Chloroplasts as a Membrane-Bound Precursor
C. Sulli and S. D. Schwartzbach
School of Biological Sciences, E207 Beadle Center, University of Nebraska, Lincoln, Nebraska 68588
The Euglena precursor to the small subunit of ribulose-1,5-bisphosphate
carboxylase/oxygenase (pSSU) is a polyprotein. To determine the transport
route from cytoplasm to chloroplast, Euglena was pulse labeled with
35S-sulfate and the organelles were separated on sucrose gradients. After a
pulse, pSSU was found in the endoplasmic reticulum (ER) and Golgi
apparatus. During a chase, ER- and Golgi-localized pSSU decreased
concomitant with the appearance of SSU in chloroplasts. SSU was not found
in pSSU-containing ER and Golgi fractions. Na2CO3 did not remove pSSU from
ER or Golgi membranes, indicating that it was an integral membrane protein.
pSSU was inserted in vitro into canine microsomes, and Na2CO3 did not
remove pSSU from the microsomal membrane. The in vivo and in vitro
experiments show that Euglena pSSU is inserted into the ER membrane and
transported as an integral membrane protein to the Golgi apparatus before
chloroplast import and polyprotein processing.
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