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THE PLANT CELL, Vol 8, Issue 11 1935-1949, Copyright © 1996 by American Society of Plant Biologists
Localized Apical Increases of Cytosolic Free Calcium Control Pollen Tube Orientation
R. Malho and A. J. Trewavas
Departamento de Biologia Vegetal, Faculdade de Ciencias de Lisboa, R. Ernesto de Vasconcelos, Bloco C2, 1780 Lisboa, Portugal
To reach the ovule, pollen tubes must undergo many changes in growth
direction. We have shown in previous work that elevation of cytosolic free
calcium ([Ca2+]c) can manipulate orientation in growing pollen tubes, but
our results suggested that [Ca2+]c changes either in the tip or in more
distal regions might regulate the critical orienting mechanism. To identify
the spatial location of the orienting motor, we combined the techniques of
ion imaging with confocal microscopy and localized photoactivation of
loaded caged Ca2+ (nitr-5) and diazo-2 (a caged Ca2+ chelator) to
manipulate [Ca2+]c in different pollen tube domains. We found that
increasing [Ca2+]c on one side of the pollen tube apex induced
reorientation of the growth axis toward that side. Similarly, a decrease in
[Ca2+]c promoted bending toward the opposite side. These effects could be
mimicked by imposing localized external gradients of an ionophore (A23187)
or a Ca2+ channel blocker (GdCl3); the pollen tubes bend toward the highest
concentration of A23187 and away from GdCl3. Manipulation of [Ca2+]c in
regions farther back from the apical zone also induced changes in growth
direction, but the new orientation was at random. We observed communication
of these distal events to the tip through a slow-moving [Ca2+]c wave. These
data show that localized changes of [Ca2+]c in the tip, which could result
from asymmetric channel activity, control the direction of pollen tube
growth.
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|
|
|
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|
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|
|
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|
|
|
|
|
|
|
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|
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|
|
|
|
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|
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|
|
|
|
|
|
|
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|
|
|
|
|
|
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123(2):
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|
|
|
|
|
|
|
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|
|
|
|
|
|
|
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120(3):
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[Full Text]
|
|
|
|
|
|
|
R. RANJEVA, A. GRAZIANA, and C. MAZARS
Plant graviperception and gravitropism: a newcomer's view
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May 1, 1999;
13(9001):
135 - 141.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Trewavas
Le Calcium, C'est la Vie: Calcium Makes Waves
Plant Physiology,
May 1, 1999;
120(1):
1 - 6.
[Full Text]
|
|
|
|
|
|
|
B. Kost, E. Lemichez, P. Spielhofer, Y. Hong, K. Tolias, C. Carpenter, and N.-H. Chua
Rac Homologues and Compartmentalized Phosphatidylinositol 4, 5-Bisphosphate Act in a Common Pathway to Regulate Polar Pollen Tube Growth
J. Cell Biol.,
April 19, 1999;
145(2):
317 - 330.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Sanders, C. Brownlee, and J. F. Harper
Communicating with Calcium
PLANT CELL,
April 1, 1999;
11(4):
691 - 706.
[Full Text]
|
|
|
|
|
|
|
V. E. Franklin-Tong
Signaling and the Modulation of Pollen Tube Growth
PLANT CELL,
April 1, 1999;
11(4):
727 - 738.
[Full Text]
|
|
|
|
|
|
|
J.A. Feijo, J. Sainhas, G.R. Hackett, J.G. Kunkel, and P.K. Hepler
Growing Pollen Tubes Possess a Constitutive Alkaline Band in the Clear Zone and a Growth-dependent Acidic Tip
J. Cell Biol.,
February 8, 1999;
144(3):
483 - 496.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Higashiyama, H. Kuroiwa, S. Kawano, and T. Kuroiwa
Guidance in Vitro of the Pollen Tube to the Naked Embryo Sac of Torenia fournieri
PLANT CELL,
December 1, 1998;
10(12):
2019 - 2032.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
W. M. Lush, F. Grieser, and M. Wolters-Arts
Directional Guidance of Nicotiana alata Pollen Tubes in Vitro and on the Stigma
Plant Physiology,
November 1, 1998;
118(3):
733 - 741.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. Moutinho, A. J. Trewavas, and R. Malhó
Relocation of a Ca2+-Dependent Protein Kinase Activity during Pollen Tube Reorientation
PLANT CELL,
September 1, 1998;
10(9):
1499 - 1510.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
H. Xu and M. C. Heath
Role of Calcium in Signal Transduction during the Hypersensitive Response Caused by Basidiospore-Derived Infection of the Cowpea Rust Fungus
PLANT CELL,
April 1, 1998;
10(4):
585 - 598.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C Digonnet, D Aldon, N Leduc, C Dumas, and M Rougier
First evidence of a calcium transient in flowering plants at fertilization
Development,
January 8, 1997;
124(15):
2867 - 2874.
[Abstract]
[PDF]
|
|
|
|
|
|
|
F. Safadi, V. S. Reddy, and A. S. N. Reddy
A Pollen-specific Novel Calmodulin-binding Protein with Tetratricopeptide Repeats
J. Biol. Chem.,
November 3, 2000;
275(45):
35457 - 35470.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A.-A. Very and J. M. Davies
Hyperpolarization-activated calcium channels at the tip of Arabidopsis root hairs
PNAS,
August 15, 2000;
97(17):
9801 - 9806.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Moutinho, P. J. Hussey, A. J. Trewavas, and R. Malho
cAMP acts as a second messenger in pollen tube growth and reorientation
PNAS,
August 28, 2001;
98(18):
10481 - 10486.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Jones, J.-J. Shen, Y. Fu, H. Li, Z. Yang, and C. S. Grierson
The Arabidopsis Rop2 GTPase Is a Positive Regulator of Both Root Hair Initiation and Tip Growth
PLANT CELL,
April 1, 2002;
14(4):
763 - 776.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
