Plant Cell Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (54)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tranel, P. J.
Right arrow Articles by Keegstra, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tranel, P. J.
Right arrow Articles by Keegstra, K.
Agricola
Right arrow Articles by Tranel, P. J.
Right arrow Articles by Keegstra, K.

THE PLANT CELL, Vol 8, Issue 11 2093-2104, Copyright © 1996 by American Society of Plant Biologists

RESEARCH ARTICLE

A Novel, Bipartite Transit Peptide Targets OEP75 to the Outer Membrane of the Chloroplastic Envelope

P. J. Tranel and K. Keegstra
Michigan State University-Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824-1312

OEP75 is an outer envelope membrane component of the chloroplastic protein import apparatus and is synthesized in the cytoplasm as a higher molecular weight precursor (prOEP75). During its own import, prOEP75 is processed first to an intermediate (iOEP75) and subsequently to the mature form (mOEP75). Experiments conducted with stromal extracts indicated that iOEP75 was generated from prOEP75 by the activity of the stromal processing peptidase. The specific processing site was determined and used to divide the prOEP75 transit peptide into N- and C-terminal domains. To determine the targeting functions of the two domains of the transit peptide and of the mature region of prOEP75, we created a deletion mutant construct from prOEP75 and chimeric constructs between domains of prOEP75 and the precursor to a small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Analysis of these constructs by in vitro chloroplastic protein import assays revealed that the transit peptide of prOEP75 is bipartite in that the N- and C-terminal portions contain chloroplastic and intraorganellar targeting information, respectively.


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
R. Patel, S.-C. Hsu, J. Bedard, K. Inoue, and P. Jarvis
The Omp85-Related Chloroplast Outer Envelope Protein OEP80 Is Essential for Viability in Arabidopsis
Plant Physiology, September 1, 2008; 148(1): 235 - 245.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. Kalanon and G. I. McFadden
The Chloroplast Protein Translocation Complexes of Chlamydomonas reinhardtii: A Bioinformatic Comparison of Toc and Tic Components in Plants, Green Algae and Red Algae
Genetics, May 1, 2008; 179(1): 95 - 112.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Miras, D. Salvi, L. Piette, D. Seigneurin-Berny, D. Grunwald, C. Reinbothe, J. Joyard, S. Reinbothe, and N. Rolland
Toc159- and Toc75-independent Import of a Transit Sequence-less Precursor into the Inner Envelope of Chloroplasts
J. Biol. Chem., October 5, 2007; 282(40): 29482 - 29492.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
M. Parsons, A. Karnataki, J. E. Feagin, and A. DeRocher
Protein Trafficking to the Apicoplast: Deciphering the Apicomplexan Solution to Secondary Endosymbiosis
Eukaryot. Cell, July 1, 2007; 6(7): 1081 - 1088.
[Full Text] [PDF]

Home page
 JCBHome page
M. Li and D. J. Schnell
Reconstitution of protein targeting to the inner envelope membrane of chloroplasts
J. Cell Biol., October 23, 2006; 175(2): 249 - 259.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
K. Inoue, A. J. Baldwin, R. L. Shipman, K. Matsui, S. M. Theg, and M. Ohme-Takagi
Complete maturation of the plastid protein translocation channel requires a type I signal peptidase
J. Cell Biol., November 7, 2005; 171(3): 425 - 430.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
C. Xu, J. Fan, J. E. Froehlich, K. Awai, and C. Benning
Mutation of the TGD1 Chloroplast Envelope Protein Affects Phosphatidate Metabolism in Arabidopsis
PLANT CELL, November 1, 2005; 17(11): 3094 - 3110.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Baldwin, A. Wardle, R. Patel, P. Dudley, S. K. Park, D. Twell, K. Inoue, and P. Jarvis
A Molecular-Genetic Study of the Arabidopsis Toc75 Gene Family
Plant Physiology, June 1, 2005; 138(2): 715 - 733.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Nada and J. Soll
Inner envelope protein 32 is imported into chloroplasts by a novel pathway
J. Cell Sci., September 1, 2004; 117(17): 3975 - 3982.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Olsson, M. Thelander, and H. Ronne
A Novel Type of Chloroplast Stromal Hexokinase Is the Major Glucose-phosphorylating Enzyme in the Moss Physcomitrella patens
J. Biol. Chem., November 7, 2003; 278(45): 44439 - 44447.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
J. Bauer, A. Hiltbrunner, P. Weibel, P.-A. Vidi, M. Alvarez-Huerta, M. D. Smith, D. J. Schnell, and F. Kessler
Essential role of the G-domain in targeting of the protein import receptor atToc159 to the chloroplast outer membrane
J. Cell Biol., December 9, 2002; 159(5): 845 - 854.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Miras, D. Salvi, M. Ferro, D. Grunwald, J. Garin, J. Joyard, and N. Rolland
Non-canonical Transit Peptide for Import into the Chloroplast
J. Biol. Chem., November 27, 2002; 277(49): 47770 - 47778.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Hirohashi, T. Hase, and M. Nakai
Maize Non-Photosynthetic Ferredoxin Precursor Is Mis-Sorted to the Intermembrane Space of Chloroplasts in the Presence of Light
Plant Physiology, April 1, 2001; 125(4): 2154 - 2163.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
J. E. Froehlich, A. Itoh, and G. A. Howe
Tomato Allene Oxide Synthase and Fatty Acid Hydroperoxide Lyase, Two Cytochrome P450s Involved in Oxylipin Metabolism, Are Targeted to Different Membranes of Chloroplast Envelope
Plant Physiology, January 1, 2001; 125(1): 306 - 317.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
T. Stahl, C. Glockmann, J. Soll, and L. Heins
Tic40, a New "Old" Subunit of the Chloroplast Protein Import Translocon
J. Biol. Chem., December 24, 1999; 274(52): 37467 - 37472.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Kouranov, H. Wang, and D. J. Schnell
Tic22 Is Targeted to the Intermembrane Space of Chloroplasts by a Novel Pathway
J. Biol. Chem., August 27, 1999; 274(35): 25181 - 25186.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. Keegstra and K. Cline
Protein Import and Routing Systems of Chloroplasts
PLANT CELL, April 1, 1999; 11(4): 557 - 570.
[Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Reumann, J. Davila-Aponte, and K. Keegstra
The evolutionary origin of the protein-translocating channel of chloroplastic envelope membranes: Identification of a cyanobacterial homolog
PNAS, January 19, 1999; 96(2): 784 - 789.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. T. Jackson, J. E. Froehlich, and K. Keegstra
The Hydrophilic Domain of Tic110, an Inner Envelope Membrane Component of the Chloroplastic Protein Translocation Apparatus, Faces the Stromal Compartment
J. Biol. Chem., June 26, 1998; 273(26): 16583 - 16588.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
T. May and J. Soll
Positive Charges Determine the Topology and Functionality of the Transmembrane Domain in the Chloroplastic Outer Envelope Protein Toc34
J. Cell Biol., May 18, 1998; 141(4): 895 - 904.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H.-m. Li and L.-J. Chen
A Novel Chloroplastic Outer Membrane-targeting Signal That Functions at Both Termini of Passenger Polypeptides
J. Biol. Chem., April 18, 1997; 272(16): 10968 - 10974.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Chen and D. J. Schnell
Insertion of the 34-kDa Chloroplast Protein Import Component, IAP34, into the Chloroplast Outer Membrane Is Dependent on Its Intrinsic GTP-binding Capacity
J. Biol. Chem., March 7, 1997; 272(10): 6614 - 6620.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. E. Froehlich, C. Benning, and P. Dormann
The Digalactosyldiacylglycerol (DGDG) Synthase DGD1 Is Inserted into the Outer Envelope Membrane of Chloroplasts in a Manner Independent of the General Import Pathway and Does Not Depend on Direct Interaction with Monogalactosyldiacylglycerol Synthase for DGDG Biosynthesis
J. Biol. Chem., August 17, 2001; 276(34): 31806 - 31812.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
X. Chen, M. D. Smith, L. Fitzpatrick, and D. J. Schnell
In Vivo Analysis of the Role of atTic20 in Protein Import into Chloroplasts
PLANT CELL, March 1, 2002; 14(3): 641 - 654.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1996 by the American Society of Plant Biologists