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THE PLANT CELL, Vol 8, Issue 12 2193-2209, Copyright © 1996 by American Society of Plant Biologists
Signal Transduction in Barley Aleurone Protoplasts Is Calcium Dependent and Independent
S. Gilroy
Biology Department, Pennsylvania State University, 208 Mueller Laboratory, University Park, Pennsylvania 16802
Gibberellic acid (GA) increases Ca2+ and calmodulin (CaM) levels in barley
aleurone cells, and abscisic acid (ABA) antagonizes the GA effect. These
alterations in cytoplasmic Ca2+ and CaM have been suggested to be central
regulators of the secretory response of the barley aleurone. Using
microinjection of caged Ca2+, Ca2+ chelators, and CaM, we mimicked or
blocked these hormonally induced changes in Ca2+ and CaM and assessed their
effects on GA and ABA action. Although mimicking GA-induced changes in Ca2+
and CaM did not mimic GA action, blocking these changes did prevent GA
stimulation of secretion. The induction of the amylase gene by GA was,
however, unaffected. Similarly, blocking the decrease in Ca2+ normally
caused by ABA in these cells blocked ABA action, except that induction of
Em gene transcription by ABA was unaffected. These results suggest that GA
and ABA signals are transduced by Ca2+- and CaM-dependent and Ca2+- and
CaM-independent systems in the aleurone cell.
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