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THE PLANT CELL, Vol 8, Issue 12 2369-2380, Copyright © 1996 by American Society of Plant Biologists
Molecular Characterization of the S Locus in Two Self-Incompatible Brassica napus Lines
K. Yu, U. Schafer, T. L. Glavin, D. R. Goring and S. J. Rothstein
Department of Molecular Biology and Genetics, University of Guelph, Guelph, Ontario, Canada N1G 2W1
In Brassica species, self-incompatibility has been mapped genetically to a
single chromosomal location. In this region, there are two closely linked
genes coding for the S locus glycoprotein (SLG) and S locus receptor kinase
(SRK). They appear to comprise the pistil component of the
self-incompatibility reaction. SLG and SRK are thought to recognize an
unknown pollen component on the incompatible pollen, and the gene encoding
this pollen component must also be linked to the SLG and SRK genes. To
further our understanding of self-incompatibility, the chromosomal region
carrying the SLG and SRK genes has been studied. The physical region
between the SLG-910 and the SRK-910 genes in the Brassica napus W1 line was
cloned, and a search for genes expressed in the anther revealed two
additional S locus genes located downstream of the SLG-910 gene. Because
these two genes are novel and are conserved at other S alleles, we
designated them as SLL1 and SLL2 (for S locus-linked genes 1 and 2,
respectively). The SLL1 gene is S locus specific, whereas the SLL2 gene is
not only present at the S locus but is also present in other parts of the
genomes in both self-incompatible and self-compatible Brassica ssp lines.
Expression of the SLL1 gene is only detectable in anthers of
self-incompatible plants and is developmentally regulated during anther
development, whereas the SLL2 gene is expressed in anthers and stigmas in
both self-incompatible and self-compatible plants, with the highest levels
of expression occurring in the stigmas. Although SLL1 and SLL2 are linked
to the S locus region, it is not clear whether these genes function in
self-incompatibility or serve some other cellular roles in pollen-pistil
functions.
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