Plant Cell Blood
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (47)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Overvoorde, P. J.
Right arrow Articles by Grimes, H. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Overvoorde, P. J.
Right arrow Articles by Grimes, H. D.
Agricola
Right arrow Articles by Overvoorde, P. J.
Right arrow Articles by Grimes, H. D.

THE PLANT CELL, Vol 8, Issue 2 271-280, Copyright © 1996 by American Society of Plant Biologists

RESEARCH ARTICLES

A Soybean Sucrose Binding Protein Independently Mediates Nonsaturable Sucrose Uptake in Yeast

P. J. Overvoorde, W. B. Frommer and H. D. Grimes
Department of Botany, Washington State University, Pullman, Washington 99164-4238

Heterologous expression of a cDNA encoding a 62-kD soybean sucrose binding protein in yeast demonstrates that this protein, independent of other plant proteins, mediates sucrose uptake across the plasma membrane. Sucrose binding protein-mediated sucrose uptake is nonsaturable up to 30 mM sucrose, is specific for sucrose, and is relatively insensitive to treatment with sulfhydryl-modifying reagents. Alteration of the external pH or pretreatment of the yeast cells with protonophores did not significantly affect the rate of 14C-sucrose uptake. This demonstrates that sucrose binding protein-mediated sucrose uptake is not dependent on H+ movement and delineates it from other plant sucrose transporters. Physiological characterization of sucrose uptake into higher plant cells has shown the presence of both saturable and nonsaturable uptake components. The nonsaturable mechanism is relatively insensitive to external pH, pretreatment with protonophores, and treatment with sulfhydryl-modifying reagents. Sucrose binding protein-mediated sucrose uptake in yeast mimics this physiologically described, but mechanistically undefined, nonsaturable sucrose uptake mechanism in higher plants. Functional characterization of the sucrose binding protein thus defines both a novel component of sucrose uptake and provides important insight into this nonsaturable sucrose uptake mechanism, which has remained enigmatic since its physiological description.


This article has been cited by other articles:


Home page
 J Exp BotHome page
J. Wang, P. K. Suen, Z.-F. Xu, and L. Jiang
A 64 kDa sucrose binding protein is membrane-associated and tonoplast-localized in developing mung bean seeds
J. Exp. Bot., January 6, 2009; (2009) ern308v1.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Y. Zhou, K. Chan, T. L. Wang, C. L. Hedley, C. E. Offler, and J. W. Patrick
Intracellular sucrose communicates metabolic demand to sucrose transporters in developing pea cotyledons
J. Exp. Bot., October 17, 2008; (2008) ern254v1.
[Abstract] [Full Text] [PDF]

Home page
 jashsHome page
D. Pozueta-Romero, P. Gonzalez, E. Etxeberria, and J. Pozueta-Romero
The Hyperbolic and Linear Phases of the Sucrose Accumulation Curve in Turnip Storage Cells Denote Carrier-mediated and Fluid Phase Endocytic Transport, Respectively
J. Amer. Soc. Hort. Sci., July 1, 2008; 133(4): 612 - 618.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. A. S. Contim, A. J. Waclawovsky, N. Delu-Filho, C. P. Pirovani, W. R. Clarindo, M. E. Loureiro, C. R. Carvalho, and E. P. B. Fontes
The soybean sucrose binding protein gene family: genomic organization, gene copy number and tissue-specific expression of the SBP2 promoter
J. Exp. Bot., December 1, 2003; 54(393): 2643 - 2653.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. de Jong and A.C. Borstlap
A plasma membrane-enriched fraction isolated from the coats of developing pea seeds contains H+-symporters for amino acids and sucrose
J. Exp. Bot., October 1, 2000; 51(351): 1671 - 1677.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. Weise, L. Barker, C. Kühn, S. Lalonde, H. Buschmann, W. B. Frommer, and J. M. Ward
A New Subfamily of Sucrose Transporters, SUT4, with Low Affinity/High Capacity Localized in Enucleate Sieve Elements of Plants
PLANT CELL, August 1, 2000; 12(8): 1345 - 1356.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
G. Ritte, J. Rosenfeld, K. Rohrig, and K. Raschke
Rates of Sugar Uptake by Guard Cell Protoplasts of Pisum sativum L. Related to the Solute Requirement for Stomatal Opening
Plant Physiology, October 1, 1999; 121(2): 647 - 656.
[Abstract] [Full Text]

Home page
 Plant CellHome page
S. Lalonde, E. Boles, H. Hellmann, L. Barker, J. W. Patrick, W. B. Frommer, and J. M. Ward
The Dual Function of Sugar Carriers: Transport and Sugar Sensing
PLANT CELL, April 1, 1999; 11(4): 707 - 726.
[Full Text]

Home page
 J. Biol. Chem.Home page
P. J. Overvoorde, W. S. Chao, and H. D. Grimes
A Plasma Membrane Sucrose-binding Protein That Mediates Sucrose Uptake Shares Structural and Sequence Similarity with Seed Storage Proteins but Remains Functionally Distinct
J. Biol. Chem., June 20, 1997; 272(25): 15898 - 15904.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1996 by the American Society of Plant Biologists