|
THE PLANT CELL, Vol 8, Issue 2 293-303, Copyright © 1996 by American Society of Plant Biologists
Localization of a Rho GTPase Implies a Role in Tip Growth and Movement of the Generative Cell in Pollen Tubes
Y. Lin, Y. Wang, J. K. Zhu and Z. Yang
Department of Plant Biology, and Plant Molecular and Biotechnology Program, Ohio State University, Columbus, Ohio 43210
The Rho family GTPases function as key molecular switches, controlling a
variety of actin-dependent cellular processes, such as the establishment of
cell polarity, cell morphogenesis, and movement in diverse eukaryotic
organisms. A novel subfamily of Rho GTPases, Rop, has been identified in
plants. Protein gel blot and RNA gel blot hybridization analyses indicated
that one of these plant Rho GTPases, Rop1, is expressed predominantly in
the male gametophyte (pollen and pollen tubes). Cell fractionation analysis
of pollen tubes showed that Rop is partitioned into soluble and particulate
fractions. The particulate Rop could be solubilized with detergents but not
with salts, indicating that it is tightly bound to membranes. The membrane
association appears to result from membrane anchoring via a geranylgeranyl
group because an in vitro isoprenylation assay demonstrated that Rop1Ps is
geranylgeranylated. Subcellular localization, using indirect
immunofluorescence and confocal microscopy, showed that Rop is highly
concentrated in the cortical region of the tube apex and in the periphery
of the generative cell. The cortical Rop protein at the apex forms a
gradient with decreasing concentration from tip to base and appears to be
associated with the plasma membrane. These results suggest that the apical
Rop GTPase may be involved in the signaling mechanism that controls the
actin-dependent tip growth of pollen tubes. Localization of the Rop GTPase
to the periphery of the generative cell is analogous to that of myosin,
suggesting that the Rop GTPase plays an important role in the modulation of
an actomyosin motor system involved in the movement of the generative cell.
This article has been cited by other articles:
|
|
|
|
 
S. Li, Y. Gu, A. Yan, E. Lord, and Z.-B. Yang
RIP1 (ROP Interactive Partner 1)/ICR1 Marks Pollen Germination Sites and May Act in the ROP1 Pathway in the Control of Polarized Pollen Growth
Mol Plant,
November 1, 2008;
1(6):
1021 - 1035.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. J. Lee, A. Szumlanski, E. Nielsen, and Z. Yang
Rho-GTPase-dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth
J. Cell Biol.,
October 22, 2008;
181(7):
1155 - 1168.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. W. Jeon, J.-U. Hwang, Y. Hwang, W.-Y. Song, Y. Fu, Y. Gu, F. Bao, D. Cho, J. M. Kwak, Z. Yang, et al.
The Arabidopsis Small G Protein ROP2 Is Activated by Light in Guard Cells and Inhibits Light-Induced Stomatal Opening
PLANT CELL,
January 1, 2008;
20(1):
75 - 87.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Zhang and S. McCormick
A distinct mechanism regulating a pollen-specific guanine nucleotide exchange factor for the small GTPase Rop in Arabidopsis thaliana
PNAS,
November 20, 2007;
104(47):
18830 - 18835.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Sorek, L. Poraty, H. Sternberg, E. Bar, E. Lewinsohn, and S. Yalovsky
Activation Status-Coupled Transient S Acylation Determines Membrane Partitioning of a Plant Rho-Related GTPase
Mol. Cell. Biol.,
March 15, 2007;
27(6):
2144 - 2154.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-S. Wang, L.-Y. Pi, X. Chen, P. K. Chakrabarty, J. Jiang, A. L. De Leon, G.-Z. Liu, L. Li, U. Benny, J. Oard, et al.
Rice XA21 Binding Protein 3 Is a Ubiquitin Ligase Required for Full Xa21-Mediated Disease Resistance
PLANT CELL,
December 1, 2006;
18(12):
3635 - 3646.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. M. Yoon, P. E. Dowd, S. Gilroy, and A. G. McCubbin
Calcium-Dependent Protein Kinase Isoforms in Petunia Have Distinct Functions in Pollen Tube Growth, Including Regulating Polarity
PLANT CELL,
April 1, 2006;
18(4):
867 - 878.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. D. Johnson, S. N. Chary, E. A. Chernoff, Q. Zeng, M. P. Running, and D. N. Crowell
Protein Geranylgeranyltransferase I Is Involved in Specific Aspects of Abscisic Acid and Auxin Signaling in Arabidopsis
Plant Physiology,
October 1, 2005;
139(2):
722 - 733.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Bi, M. Baudry, J. Liu, Y. Yao, L. Fu, F. Brucher, and G. Lynch
Inhibition of Geranylgeranylation Mediates the Effects of 3-Hydroxy-3-methylglutaryl (HMG)-CoA Reductase Inhibitors on Microglia
J. Biol. Chem.,
November 12, 2004;
279(46):
48238 - 48245.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Gupta and S. Luan
Redox Control of Protein Tyrosine Phosphatases and Mitogen-Activated Protein Kinases in Plants
Plant Physiology,
July 1, 2003;
132(3):
1149 - 1152.
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Camacho and R. Malho
Endo/exocytosis in the pollen tube apex is differentially regulated by Ca2+ and GTPases
J. Exp. Bot.,
January 1, 2003;
54(380):
83 - 92.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Gu, V. Vernoud, Y. Fu, and Z. Yang
ROP GTPase regulation of pollen tube growth through the dynamics of tip-localized F-actin
J. Exp. Bot.,
January 1, 2003;
54(380):
93 - 101.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Nakanomyo, B. Kost, N.-H. Chua, and H. Fukuda
Preferential and Asymmetrical Accumulation of a Rac Small GTPase mRNA in Differentiating Xylem Cells of Zinnia elegans
Plant Cell Physiol.,
December 15, 2002;
43(12):
1484 - 1492.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Lavy, K. Bracha-Drori, H. Sternberg, and S. Yalovsky
A Cell-Specific, Prenylation-Independent Mechanism Regulates Targeting of Type II RACs
PLANT CELL,
October 1, 2002;
14(10):
2431 - 2450.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Yang
Small GTPases: Versatile Signaling Switches in Plants
PLANT CELL,
May 1, 2002;
14(90001):
S375 - 388.
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Fu, H. Li, and Z. Yang
The ROP2 GTPase Controls the Formation of Cortical Fine F-Actin and the Early Phase of Directional Cell Expansion during Arabidopsis Organogenesis
PLANT CELL,
April 1, 2002;
14(4):
777 - 794.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Wu, Y. Gu, S. Li, and Z. Yang
A Genome-Wide Analysis of Arabidopsis Rop-Interactive CRIB Motif-Containing Proteins That Act as Rop GTPase Targets
PLANT CELL,
December 1, 2001;
13(12):
2841 - 2856.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Vidali, S. T. McKenna, and P. K. Hepler
Actin Polymerization Is Essential for Pollen Tube Growth
Mol. Biol. Cell,
August 1, 2001;
12(8):
2534 - 2545.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Caldelari, H. Sternberg, M. Rodriguez-Concepcion, W. Gruissem, and S. Yalovsky
Efficient Prenylation by a Plant Geranylgeranyltransferase-I Requires a Functional CaaL Box Motif and a Proximal Polybasic Domain
Plant Physiology,
August 1, 2001;
126(4):
1416 - 1429.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Li, J.-J. Shen, Z.-L. Zheng, Y. Lin, and Z. Yang
The Rop GTPase Switch Controls Multiple Developmental Processes in Arabidopsis
Plant Physiology,
June 1, 2001;
126(2):
670 - 684.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Fu, G. Wu, and Z. Yang
Rop GTPase-dependent Dynamics of Tip-localized F-Actin Controls Tip Growth in Pollen Tubes
J. Cell Biol.,
March 5, 2001;
152(5):
1019 - 1032.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Lin, D. F. Seals, S. K. Randall, and Z. Yang
Dynamic Localization of Rop GTPases to the Tonoplast during Vacuole Development
Plant Physiology,
January 1, 2001;
125(1):
241 - 251.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
G. Wu, H. Li, and Z. Yang
Arabidopsis RopGAPs Are a Novel Family of Rho GTPase-Activating Proteins that Require the Cdc42/Rac-Interactive Binding Motif for Rop-Specific GTPase Stimulation
Plant Physiology,
December 1, 2000;
124(4):
1625 - 1636.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. Park, H.-J. Choi, S. Lee, T. Lee, Z. Yang, and Y. Lee
Rac-Related GTP-Binding Protein in Elicitor-Induced Reactive Oxygen Generation by Suspension-Cultured Soybean Cells
Plant Physiology,
October 1, 2000;
124(2):
725 - 732.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C. A. R. Hurtado, J.-M. Beckerich, C. Gaillardin, and R. A. Rachubinski
A Rac Homolog Is Required for Induction of Hyphal Growth in the Dimorphic Yeast Yarrowia lipolytica
J. Bacteriol.,
May 1, 2000;
182(9):
2376 - 2386.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
H. Li, Y. Lin, R. M. Heath, M. X. Zhu, and Z. Yang
Control of Pollen Tube Tip Growth by a Rop GTPase –Dependent Pathway That Leads to Tip-Localized Calcium Influx
PLANT CELL,
September 1, 1999;
11(9):
1731 - 1742.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
L. Ma, X. Xu, S. Cui, and D. Sun
The Presence of a Heterotrimeric G Protein and Its Role in Signal Transduction of Extracellular Calmodulin in Pollen Germination and Tube Growth
PLANT CELL,
July 1, 1999;
11(7):
1351 - 1364.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
R. B. Meagher, E. C. McKinney, and M. K. Kandasamy
Isovariant Dynamics Expand and Buffer the Responses of Complex Systems: The Diverse Plant Actin Gene Family
PLANT CELL,
June 1, 1999;
11(6):
995 - 1006.
[Full Text]
|
|
|
|
|
|
|
B. Kost, E. Lemichez, P. Spielhofer, Y. Hong, K. Tolias, C. Carpenter, and N.-H. Chua
Rac Homologues and Compartmentalized Phosphatidylinositol 4, 5-Bisphosphate Act in a Common Pathway to Regulate Polar Pollen Tube Growth
J. Cell Biol.,
April 19, 1999;
145(2):
317 - 330.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. H. Battey, N. C. James, A. J. Greenland, and C. Brownlee
Exocytosis and Endocytosis
PLANT CELL,
April 1, 1999;
11(4):
643 - 660.
[Full Text]
|
|
|
|
|
|
|
V. E. Franklin-Tong
Signaling and the Modulation of Pollen Tube Growth
PLANT CELL,
April 1, 1999;
11(4):
727 - 738.
[Full Text]
|
|
|
|
|
|
|
A. E. Trotochaud, T. Hao, G. Wu, Z. Yang, and S. E. Clark
The CLAVATA1 Receptor-like Kinase Requires CLAVATA3 for Its Assembly into a Signaling Complex That Includes KAPP and a Rho-Related Protein
PLANT CELL,
March 1, 1999;
11(3):
393 - 406.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Li, G. Wu, D. Ware, K. R. Davis, and Z. Yang
Arabidopsis Rho-Related GTPases: Differential Gene Expression in Pollen and Polar Localization in Fission Yeast
Plant Physiology,
October 1, 1998;
118(2):
407 - 417.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. Moutinho, A. J. Trewavas, and R. Malhó
Relocation of a Ca2+-Dependent Protein Kinase Activity during Pollen Tube Reorientation
PLANT CELL,
September 1, 1998;
10(9):
1499 - 1510.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
D. N. Crowell, S. E. Sen, and S. K. Randall
Prenylcysteine alpha -Carboxyl Methyltransferase in Suspension-Cultured Tobacco Cells
Plant Physiology,
September 1, 1998;
118(1):
115 - 123.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. R. Clarke, C. J. Staiger, B. C. Gibbon, and V. E. Franklin-Tong
A Potential Signaling Role for Profilin in Pollen of Papaver rhoeas
PLANT CELL,
June 1, 1998;
10(6):
967 - 980.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. Muschietti, Y. Eyal, and S. McCormick
Pollen Tube Localization Implies a Role in Pollen–Pistil Interactions for the Tomato Receptor-like Protein Kinases LePRK1 and LePRK2
PLANT CELL,
March 1, 1998;
10(3):
319 - 330.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U Folkers, J Berger, and M Hulskamp
Cell morphogenesis of trichomes in Arabidopsis: differential control of primary and secondary branching by branch initiation regulators and cell growth
Development,
January 10, 1997;
124(19):
3779 - 3786.
[Abstract]
[PDF]
|
|
|
|
|
|
|
E. C. Ziegelhoffer, L. J. Medrano, and E. M. Meyerowitz
Cloning of the Arabidopsis WIGGUM gene identifies a role for farnesylation in meristem development
PNAS,
June 20, 2000;
97(13):
7633 - 7638.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A.-A. Very and J. M. Davies
Hyperpolarization-activated calcium channels at the tip of Arabidopsis root hairs
PNAS,
August 15, 2000;
97(17):
9801 - 9806.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Jones, J.-J. Shen, Y. Fu, H. Li, Z. Yang, and C. S. Grierson
The Arabidopsis Rop2 GTPase Is a Positive Regulator of Both Root Hair Initiation and Tip Growth
PLANT CELL,
April 1, 2002;
14(4):
763 - 776.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
