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THE PLANT CELL, Vol 8, Issue 3 403-416, Copyright © 1996 by American Society of Plant Biologists
The Critical Requirement for Linolenic Acid Is Pollen Development, Not Photosynthesis, in an Arabidopsis Mutant
M. McConn and J. Browse
Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340
The very high proportions of trienoic fatty acids found in chloroplast
membranes of all higher plants suggest that these lipid structures might be
essential for photosynthesis. We report here on the production of
Arabidopsis triple mutants that contain negligible levels of trienoic fatty
acids. Photosynthesis at 22[deg]C was barely affected, and vegetative
growth of the mutants was identical with that of the wild type,
demonstrating that any requirement for trienoic acyl groups in membrane
structure and function is relatively subtle. Although vegetative growth and
development were unaffected, the triple mutants are male sterlle and
produce no seed under normal conditions. Comparisons of pollen development
in wild-type and triple mutant flowers established that pollen grains in
the mutant developed to the tricellular stage. Exogenous applications of
[alpha]-llnolenate or jasmonate restored fertility. Taken together, the
results demonstrate that the critical role of trienoic acids in the life
cycle of plants is as the precursor of oxylipin, a signaling compound that
regulates final maturation processes and the release of pollen.
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