Plant Cell SoftGenetics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (83)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stein, J. C.
Right arrow Articles by Nasrallah, J. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stein, J. C.
Right arrow Articles by Nasrallah, J. B.
Agricola
Right arrow Articles by Stein, J. C.
Right arrow Articles by Nasrallah, J. B.

THE PLANT CELL, Vol 8, Issue 3 429-445, Copyright © 1996 by American Society of Plant Biologists

RESEARCH ARTICLES

SRK, the Stigma-Specific S Locus Receptor Kinase of Brassica, Is Targeted to the Plasma Membrane in Transgenic Tobacco

J. C. Stein, R. Dixit, M. E. Nasrallah and J. B. Nasrallah
Section of Plant Biology, Division of Biological Sciences, Cornell University, Ithaca, New York 14853

The S locus receptor kinase (SRK) gene is one of two S locus genes required for the self-incompatibility response in Brassica. We have identified the product of the SRK6 gene in B. oleracea stigmas and have shown that it has characteristics of an integral membrane protein. When expressed in transgenic tobacco, SRK6 is glycosylated and targeted to the plasma membrane. These results provide definitive biochemical evidence for the existence in plants of a plasma membrane-localized transmembrane protein kinase with a known cell-cell recognition function. The timing of SRK expression in stigmas follows a time course similar to that previously described for another S locus-linked gene, the S locus glycoprotein (SLG) gene, and correlates with the ability of stigmas to mount a self-incompatibility response. Based on SRK6 promoter studies, the site of gene expression overlaps with that of SLG and exhibits predominant expression in the stigmatic papillar cells. Although reporter gene studies indicated that the SRK promoter was active in pollen, SRK protein was not detected in pollen, suggesting that SRK functions as a cell surface receptor exclusively in the papillar cells of the stigma.


This article has been cited by other articles:


Home page
 GeneticsHome page
J. W. Busch, J. Sharma, and D. J. Schoen
Molecular Characterization of Lal2, an SRK-Like Gene Linked to the S-Locus in the Wild Mustard Leavenworthia alabamica
Genetics, April 1, 2008; 178(4): 2055 - 2067.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Ouelhadj, M. Kaminski, M. Mittag, and K. Humbeck
Receptor-like protein kinase HvLysMR1 of barley (Hordeum vulgare L.) is induced during leaf senescence and heavy metal stress
J. Exp. Bot., April 1, 2007; 58(6): 1381 - 1396.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Tor, D. Brown, A. Cooper, A. Woods-Tor, K. Sjolander, J. D.G. Jones, and E. B. Holub
Arabidopsis Downy Mildew Resistance Gene RPP27 Encodes a Receptor-Like Protein Similar to CLAVATA2 and Tomato Cf-9
Plant Physiology, June 1, 2004; 135(2): 1100 - 1112.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Chookajorn, A. Kachroo, D. R. Ripoll, A. G. Clark, and J. B. Nasrallah
Inaugural Article: Specificity determinants and diversification of the Brassica self-incompatibility pollen ligand
PNAS, January 27, 2004; 101(4): 911 - 917.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S.-H. Shiu and A. B. Bleecker
Expansion of the Receptor-Like Kinase/Pelle Gene Family and Receptor-Like Proteins in Arabidopsis
Plant Physiology, June 1, 2003; 132(2): 530 - 543.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
B. P. Kemp and J. Doughty
Just how complex is the BrassicaS-receptor complex?
J. Exp. Bot., January 1, 2003; 54(380): 157 - 168.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
M. E. Nasrallah, P. Liu, and J. B. Nasrallah
Generation of Self-Incompatible Arabidopsis thaliana by Transfer of Two S Locus Genes from A. lyrata
Science, July 12, 2002; 297(5579): 247 - 249.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Brueggeman, N. Rostoks, D. Kudrna, A. Kilian, F. Han, J. Chen, A. Druka, B. Steffenson, and A. Kleinhofs
The barley stem rust-resistance gene Rpg1 is a novel disease-resistance gene with homology to receptor kinases
PNAS, July 9, 2002; 99(14): 9328 - 9333.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. Kachroo, M. E. Nasrallah, and J. B. Nasrallah
Self-Incompatibility in the Brassicaceae: Receptor-Ligand Signaling and Cell-to-Cell Communication
PLANT CELL, May 1, 2002; 14(90001): S227 - 238.
[Full Text] [PDF]

Home page
 Sci SignalHome page
S.-H. Shiu and A. B. Bleecker
Plant Receptor-Like Kinase Gene Family: Diversity, Function, and Signaling
Sci. Signal., December 18, 2001; 2001(113): re22 - re22.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
C. Miege, V. Ruffio-Chable, M. H. Schierup, D. Cabrillac, C. Dumas, T. Gaude, and J. M. Cock
Intrahaplotype Polymorphism at the Brassica S Locus
Genetics, October 1, 2001; 159(2): 811 - 822.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
A. Kachroo, C. R. Schopfer, M. E. Nasrallah, and J. B. Nasrallah
Allele-Specific Receptor-Ligand Interactions in Brassica Self-Incompatibility
Science, September 7, 2001; 293(5536): 1824 - 1826.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
E. Yamamoto and H. T. Knap
Soybean Receptor-like Protein Kinase Genes: Paralogous Divergence of a Gene Family
Mol. Biol. Evol., August 1, 2001; 18(8): 1522 - 1531.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Z. Chen
A Superfamily of Proteins with Novel Cysteine-Rich Repeats
Plant Physiology, June 1, 2001; 126(2): 473 - 476.
[Full Text] [PDF]

Home page
 Plant CellHome page
M. Kusaba, K. Dwyer, J. Hendershot, J. Vrebalov, J. B. Nasrallah, and M. E. Nasrallah
Self-Incompatibility in the Genus Arabidopsis: Characterization of the S Locus in the Outcrossing A. lyrata and Its Autogamous Relative A. thaliana
PLANT CELL, March 1, 2001; 13(3): 627 - 643.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
R. Dixit, M. E. Nasrallah, and J. B. Nasrallah
Post-Transcriptional Maturation of the S Receptor Kinase of Brassica Correlates with Co-Expression of the S-Locus Glycoprotein in the Stigmas of Two Brassica Strains and in Transgenic Tobacco Plants
Plant Physiology, September 1, 2000; 124(1): 297 - 312.
[Abstract] [Full Text]

Home page
 ScienceHome page
C. R. Schopfer, M. E. Nasrallah, and J. B. Nasrallah
The Male Determinant of Self-Incompatibility in Brassica
Science, November 26, 1999; 286(5445): 1697 - 1700.
[Abstract] [Full Text]

Home page
 Plant CellHome page
Y. Cui, N. Brugière, L. Jackman, Y.-M. Bi, and S. J. Rothstein
Structural and Transcriptional Comparative Analysis of the S Locus Regions in Two Self-Incompatible Brassica napus Lines
PLANT CELL, November 1, 1999; 11(11): 2217 - 2232.
[Abstract] [Full Text]

Home page
 GeneticsHome page
G. Suzuki, N. Kai, T. Hirose, K. Fukui, T. Nishio, S. Takayama, A. Isogai, M. Watanabe, and K. Hinata
Genomic Organization of the S Locus: Identification and Characterization of Genes in SLG/SRK Region of S9 Haplotype of Brassica campestris (syn. rapa)
Genetics, September 1, 1999; 153(1): 391 - 400.
[Abstract] [Full Text]

Home page
 Plant CellHome page
D. Cabrillac, V. Delorme, J. Garin, V. Ruffio-Châble, J.-L. Giranton, C. Dumas, T. Gaude, and J. M. Cock
The S15 Self-Incompatibility Haplotype in Brassica oleracea Includes Three S Gene Family Members Expressed in Stigmas
PLANT CELL, May 1, 1999; 11(5): 971 - 986.
[Abstract] [Full Text]

Home page
 DevelopmentHome page
G. Zinkl, B. Zwiebel, D. Grier, and D Preuss
Pollen-stigma adhesion in Arabidopsis: a species-specific interaction mediated by lipophilic molecules in the pollen exine
Development, January 12, 1999; 126(23): 5431 - 5440.
[Abstract] [PDF]

Home page
 Plant CellHome page
J. Doughty, S. Dixon, S. J. Hiscock, A. C. Willis, I. A. P. Parkin, and H. G. Dickinson
PCP-A1, a Defensin-like Brassica Pollen Coat Protein That Binds the S Locus Glycoprotein, Is the Product of Gametophytic Gene Expression
PLANT CELL, August 1, 1998; 10(8): 1333 - 1348.
[Abstract] [Full Text]

Home page
 Plant CellHome page
G.-L. Wang, D.-L. Ruan, W.-Y. Song, S. Sideris, L. Chen, L.-Y. Pi, S. Zhang, Z. Zhang, C. Fauquet, B. S. Gaut, et al.
Xa21D Encodes a Receptor-like Molecule with a Leucine-Rich Repeat Domain That Determines Race-Specific Recognition and Is Subject to Adaptive Evolution
PLANT CELL, May 1, 1998; 10(5): 765 - 780.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Gu, M. Mazzurco, W. Sulaman, D. D. Matias, and D. R. Goring
Binding of an arm repeat protein to the kinase domain of the S-locus receptor kinase
PNAS, January 6, 1998; 95(1): 382 - 387.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. B. Nasrallah
Evolution of the Brassica self-incompatibility locus: A look into S-locus gene polymorphisms
PNAS, September 2, 1997; 94(18): 9516 - 9519.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Kusaba, T. Nishio, Y. Satta, K. Hinata, and D. Ockendon
Striking sequence similarity in inter- and intra-specific comparisons of class I SLG alleles from Brassica oleracea and Brassica campestris: Implications for the evolution and recognition mechanism
PNAS, July 8, 1997; 94(14): 7673 - 7678.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
S. Ikeda, J. B. Nasrallah, R. Dixit, S. Preiss, and M. E. Nasrallah
An Aquaporin-Like Gene Required for the Brassica Self-Incompatibility Response
Science, June 6, 1997; 276(5318): 1564 - 1566.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Cui, Y.-M. Bi, N. Brugiere, M. Arnoldo, and S. J. Rothstein
The S locus glycoprotein and the S receptor kinase are sufficient for self-pollen rejection in Brassica
PNAS, March 28, 2000; 97(7): 3713 - 3717.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J.-L. Giranton, C. Dumas, J. M. Cock, and T. Gaude
The integral membrane S-locus receptor kinase of Brassica has serine/threonine kinase activity in a membranous environment and spontaneously forms oligomers in planta
PNAS, March 28, 2000; 97(7): 3759 - 3764.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1996 by the American Society of Plant Biologists