Plant Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bellincampi, D.
Right arrow Articles by Lorenzo, G. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bellincampi, D.
Right arrow Articles by Lorenzo, G. D.
Agricola
Right arrow Articles by Bellincampi, D.
Right arrow Articles by Lorenzo, G. D.

THE PLANT CELL, Vol 8, Issue 3 477-487, Copyright © 1996 by American Society of Plant Biologists

RESEARCH ARTICLES

Oligogalacturonides Prevent Rhizogenesis in rolB-Transformed Tobacco Explants by Inhibiting Auxin-Induced Expression of the rolB Gene

D. Bellincampi, M. Cardarelli, D. Zaghi, G. Serino, G. Salvi, C. Gatz, F. Cervone, M. M. Altamura, P. Costantino and G. D. Lorenzo
Dipartimento di Biologia Vegetale, Universita di Roma "La Sapienza," Piazzale Aldo Moro 5, 00185 Rome, Italy

Oligogalacturonides elicit several defense responses and regulate different aspects of growth and development in plants. Many of the development-related effects of oligogalacturonides appear to be amenable to an auxin antagonist activity of these oligosaccharins. To clarify the role of oligogalacturonides in antagonizing auxin, we analyzed their effect on root formation in leaf explants of tobacco harboring the plant oncogene rolB. We show here that oligogalacturonides are capable of inhibiting root morphogenesis driven by rolB in transgenic leaf explants when this process requires exogenous auxin. Because rolB expression is induced by auxin and dramatically alters the response to this hormone in transformed plant cells, the inhibiting effect of oligogalacturonides could be exerted on the induction of rolB and/or at some other auxin-requiring step(s) in rhizogenesis. We show that oligogalacturonides antagonize auxin primarily because they strongly inhibit auxin-regulated transcriptional activation of a rolB-[beta]-glucuronidase gene fusion in both leaf explants and cultured leaf protoplasts. In contrast, oligogalacturonides do not inhibit rhizogenesis when rolB transcriptional activation is made independent of auxin, as shown by the lack of inhibition of root formation in leaf explants containing rolB driven by a tetracycline-inducible promoter.


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
R. Galletti, C. Denoux, S. Gambetta, J. Dewdney, F. M. Ausubel, G. De Lorenzo, and S. Ferrari
The AtrbohD-Mediated Oxidative Burst Elicited by Oligogalacturonides in Arabidopsis Is Dispensable for the Activation of Defense Responses Effective against Botrytis cinerea
Plant Physiology, November 1, 2008; 148(3): 1695 - 1706.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Ferrari, R. Galletti, D. Pontiggia, C. Manfredini, V. Lionetti, D. Bellincampi, F. Cervone, and G. De Lorenzo
Transgenic Expression of a Fungal endo-Polygalacturonase Increases Plant Resistance to Pathogens and Reduces Auxin Sensitivity
Plant Physiology, February 1, 2008; 146(2): 669 - 681.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
R. Moscatiello, P. Mariani, D. Sanders, and F. J. M. Maathuis
Transcriptional analysis of calcium-dependent and calcium-independent signalling pathways induced by oligogalacturonides
J. Exp. Bot., August 1, 2006; 57(11): 2847 - 2865.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Spadoni, O. Zabotina, A. Di Matteo, J. D. Mikkelsen, F. Cervone, G. De Lorenzo, B. Mattei, and D. Bellincampi
Polygalacturonase-Inhibiting Protein Interacts with Pectin through a Binding Site Formed by Four Clustered Residues of Arginine and Lysine
Plant Physiology, June 1, 2006; 141(2): 557 - 564.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. D'Ovidio, A. Raiola, C. Capodicasa, A. Devoto, D. Pontiggia, S. Roberti, R. Galletti, E. Conti, D. O'Sullivan, and G. De Lorenzo
Characterization of the Complex Locus of Bean Encoding Polygalacturonase-Inhibiting Proteins Reveals Subfunctionalization for Defense against Fungi and Insects
Plant Physiology, August 1, 2004; 135(4): 2424 - 2435.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. D. Spiro, J. F. Bowers, and D. J. Cosgrove
A Comparison of Oligogalacturonide- and Auxin-Induced Extracellular Alkalinization and Growth Responses in Roots of Intact Cucumber Seedlings
Plant Physiology, October 1, 2002; 130(2): 895 - 903.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Bellincampi, N. Dipierro, G. Salvi, F. Cervone, and G. De Lorenzo
Extracellular H2O2 Induced by Oligogalacturonides Is Not Involved in the Inhibition of the Auxin-Regulated rolB Gene Expression in Tobacco Leaf Explants
Plant Physiology, April 1, 2000; 122(4): 1379 - 1386.
[Abstract] [Full Text]

Home page
 Plant CellHome page
K. Baumann, A. De Paolis, P. Costantino, and G. Gualberti
The DNA Binding Site of the Dof Protein NtBBF1 Is Essential for Tissue-Specific and Auxin-Regulated Expression of the rolB Oncogene in Plants
PLANT CELL, March 1, 1999; 11(3): 323 - 334.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1996 by the American Society of Plant Biologists