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THE PLANT CELL, Vol 8, Issue 4 701-711, Copyright © 1996 by American Society of Plant Biologists
Cloning and Functional Expression of a Plant Voltage-Dependent Chloride Channel
C. Lurin, D. Geelen, H. Barbier-Brygoo, J. Guern and C. Maurel
Institut des Sciences Vegetales, Centre National de la Recherche Scientifique (CNRS), Bat 22, avenue de la Terrasse, F-91198 Gif sur Yvette Cedex, France
Plant cell membrane anion channels participate in basic physiological
functions, such as cell volume regulation and signal transduction. However,
nothing is known about their molecular structure. Using a polymerase chain
reaction strategy, we have cloned a tobacco cDNA (CIC-Nt1) encoding a
780-amino acid protein with several putative transmembrane domains. CIC-Nt1
displays 24 to 32% amino acid identity with members of the animal
voltage-dependent chloride channel (CIC) family, whose archetype is CIC-0
from the Torpedo marmorata electric organ. Injection of CIC-Nt1
complementary RNA into Xenopus oocytes elicited slowly activating inward
currents upon membrane hyperpolarization more negative than -120 mV. These
currents were carried mainly by anions, modulated by extracellular anions,
and totally blocked by 10 mM extracellular calcium. The identification of
CIC-Nt1 extends the CIC family to higher plants and provides a molecular
probe for the study of voltage-dependent anion channels in plants.
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