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THE PLANT CELL, Vol 8, Issue 7 1193-1207, Copyright © 1996 by American Society of Plant Biologists
A Nuclear Mutant of Arabidopsis with Impaired Stability on Distinct Transcripts of the Plastid psbB, psbD/C, ndhH, and ndhC Operons
J. Meurer, A. Berger and P. Westhoff
Institut fur Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universitat, Universitatsstrasse 1, D-40225 Dusseldorf, Germany
The high-chlorophyll fluorescence photosynthesis mutant hcf109 of
Arabidopsis was characterized in detail to gain insights into the
regulatory mechanism of RNA processing in higher plants. By using electron
transport, chlorophyll fluorescence, and immunoblot studies, we assigned
the mutational lesion to photosystems I and II and the plastid NAD(P)H
dehydrogenase complex. The functional pleiotropy was reflected in RNA
deficiencies. Although all nuclear-encoded photosynthetic RNAs analyzed
revealed no difference in size or steady state level between mutant and
wild type, the RNA patterns of the plastome-encoded
psbB-psbT-psbH-petB-petD, psbD-psbC-ycf9, ndhC-ndhK-ndhJ, and
ndhH-ndhA-ndhI-ndhG-ndhE-psaC-ndhD transcription units were severely
disturbed. These operons encode subunits of photosystems I (psa) and II
(psb), the cytochrome b6f complex (pet), the plastid NAD(P)H dehydrogenase
(ndh), and the unidentified open reading frame ycf9. With the exception of
the ndhC operon, the RNA deficiencies observed were specific and restricted
to particular segments of the psbB, psbD/C, and ndhH operons, that is, the
psbB-psbT, ycf9, and psaC regions. Run-on transcription studies with
isolated chloroplasts showed that the failure of these transcripts to
accumulate was due to RNA stability and not transcription. Other
polycistronic transcription units analyzed were not affected by the
mutation. This result indicates that the trans-regulatory factor encoded by
the hcf109 gene is not a general RNA stability factor but that it
specifically controls the stability of only these distinct transcripts.
Because the hcf109 locus was mapped at a distance <0.1 centimorgans from
the phytochrome C gene, its molecular characterization by positional
cloning is possible.
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