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THE PLANT CELL, Vol 9, Issue 1 85-95, Copyright © 1997 by American Society of Plant Biologists
A Mutant S3 RNase of Petunia inflata Lacking RNase Activity Has an Allele-Specific Dominant Negative Effect on Self-Incompatibility Interactions
A. G. McCubbin, Y. Y. Chung and Th. Kao
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802-4500
Gametophytic self-incompatibility in the Solanaceae is controlled by a
multiallelic locus called the S locus. Growth of pollen tubes in the pistil
is inhibited when the pollen has one of the two S alleles carried by the
pistil. The products of a number of pistil S alleles[mdash]S proteins or S
RNases[mdash]have been identified, and their role in controlling the
pistil's ability to reject self-pollen has been positively established. In
contrast, the existence of pollen S allele products has so far been
inferred entirely from genetic evidence. Here, we introduced a modified S3
gene of Petunia inflata encoding an S3 RNase lacking RNase activity into P.
inflata plants of the S2S3 genotype to determine whether the production of
the mutant protein, designated S3(H93R), would have any effect on the
ability of the transgenic plants to reject S2 and S3 pollen. Analysis of
the self-incompatibility behavior of 49 primary transgenic plants and the
progeny of three plants (H30, H37, and H40) that produced S3(H93R) in
addition to producing wild-type levels of endogenous S2 and S3 RNases
revealed that S3(H93R) had a dominant negative effect on the function of
the S3 RNase in rejecting self-pollen; however, it had no effect on the
function of the S2 RNase. One likely explanation of the results is that
S3(H93R) competes with the S3 RNase for binding to a common molecule, which
is presumably the product of the pollen S3 allele.
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