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THE PLANT CELL, Vol 9, Issue 12 2119-2134, Copyright © 1997 by American Society of Plant Biologists
Regulatory Sequences of Arabidopsis Drive Reporter Gene Expression in Nematode Feeding Structures
N. Barthels, F. M. van der Lee, J. Klap, OJM. Goddijn, M. Karimi, P. Puzio, FMW. Grundler, S. A. Ohl, K. Lindsey, L. Robertson, W. M. Robertson, M. Van Montagu, G. Gheysen and P. C. Sijmons
Laboratorium voor Genetica, Department of Genetics, Flanders Interuniversity Institute for Biotechnology (VIB), Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium
In the quest for plant regulatory sequences capable of driving
nematode-triggered effector gene expression in feeding structures, we show
that promoter tagging is a valuable tool. A large collection of transgenic
Arabidopsis plants was generated. They were transformed with a
[beta]-glucuronidase gene functioning as a promoter tag. Three T-DNA
constructs, pGV1047, p[delta]gusBin19, and pMOG553, were used. Early
responses to nematode invasion were of primary interest. Six lines
exhibiting [beta]-glucuronidase activity in syncytia induced by the beet
cyst nematode were studied. Reporter gene activation was also identified in
galls induced by root knot and ectoparasitic nematodes. Time-course studies
revealed that all six tags were differentially activated during the
development of the feeding structure. T-DNA-flanking regions responsible
for the observed responses after nematode infection were isolated and
characterized for promoter activity.
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