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THE PLANT CELL, Vol 9, Issue 3 355-365, Copyright © 1997 by American Society of Plant Biologists
Molecular Dissection of the AGAMOUS Control Region Shows That cis Elements for Spatial Regulation Are Located Intragenically
L. E. Sieburth and E. M. Meyerowitz
Division of Biology 156-29, California Institute of Technology, Pasadena, California 91125
AGAMOUS (AG) is an Arabidopsis MADS box gene required for the normal
development of the internal two whorls of the flower. AG RNA accumulates in
distinct patterns early and late in flower development, and several genes
have been identified as regulators of AG gene expression based on altered
AG RNA accumulation in mutants. To understand AG regulatory circuits, we
are now identifying cis regulatory domains by characterizing
AG::[beta]-glucuronidase (GUS) gene fusions. These studies show that a
normal AG::GUS staining pattern is conferred by a 9.8-kb region
encompassing 6 kb of upstream sequences and 3.8 kb of intragenic sequences.
Constructs lacking the 3.8-kb intragenic sequences confer a GUS staining
pattern that deviates both spatially and temporally from normal AG
expression. The GUS staining patterns in the mutants for the three negative
regulators of AG, apetala2, leunig, and curly leaf, showed the predicted
change of expression for the construct containing the intragenic sequences,
but no significant change was observed for the constructs lacking this
intragenic region. These results suggest that intragenic sequences are
essential for AG regulation and that these intragenic sequences contain the
ultimate target sites for at least some of the known regulatory molecules.
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Compartmentalized expression of two structurally and functionally distinct 4-coumarate:CoA ligase genes in aspen (Populus tremuloides)
PNAS,
April 28, 1998;
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[Abstract]
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L. Sieburth, G. Drews, and E. Meyerowitz
Non-autonomy of AGAMOUS function in flower development: use of a Cre/loxP method for mosaic analysis in Arabidopsis
Development,
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[Abstract]
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J. Tilly, D. Allen, and T Jack
The CArG boxes in the promoter of the Arabidopsis floral organ identity gene APETALA3 mediate diverse regulatory effects
Development,
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[Abstract]
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J. Conner and Z. Liu
LEUNIG, a putative transcriptional corepressor that regulates AGAMOUS expression during flower development
PNAS,
November 7, 2000;
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[Abstract]
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