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THE PLANT CELL, Vol 9, Issue 4 653-664, Copyright © 1997 by American Society of Plant Biologists
A Functional Homolog of Mammalian Protein Kinase C Participates in the Elicitor-Induced Defense Response in Potato
R. Subramaniam, C. Despres and N. Brisson
Department of Biochemistry, Universite de Montreal, Montreal, Canada H3C 3J7
The elicitor-induced activation of the potato pathogenesis-related gene
PR-10a is positively controlled by a protein kinase(s) that affects the
binding of the nuclear factors PBF-1 (for PR-10a binding factor-1) and
PBF-2 to an elicitor response element (ERE). In this study, we have
identified a kinase that has properties similar to the conventional
isoenzymes of the mammalian protein kinase C (PKC) family. The treatment of
potato tuber discs with specific inhibitors of PKC abolished the
elicitor-induced binding of the nuclear factor PBF-2 to the ERE. This
correlated with a reduction in the accumulation of the PR-10a protein. In
contrast, treatment of the tuber discs with 12-O-tetradecanoylphorbol
13-acetate (TPA), an activator of PKC, led to an increase in binding of
PBF-2 to the ERE and the corresponding increase in the level of the PR-10a
protein, mimicking the effect seen with the elicitor arachidonic acid.
Biochemical characterization of proteins extracted from the particulate
fraction of potato tubers demonstrated that a kinase belonging to the
conventional isoforms of PKC is present. This was confirmed by
immunoprecipitation with antibodies specific to the conventional isoforms
of human PKC and in-gel kinase assays. The ability of the
immunoprecipitates to phosphorylate the [alpha]-peptide (a PKC specific
substrate) in the presence of the coactivators calcium, phosphatidylserine,
and TPA strongly suggested that the immunoprecipitated kinase is similar to
the kinase characterized biochemically. Finally, the similar effects of the
various modulators of PKC activity on the elicitor-induced resistance
against a compatible race of Phytophthora infestans implicate this kinase
in the overall defense response in potato.
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