Plant Cell Applied Biosystems SYBR(R) Cells-to-CT(TM) Kits
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (117)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Suzuki, M.
Right arrow Articles by McCarty, D. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Suzuki, M.
Right arrow Articles by McCarty, D. R.
Agricola
Right arrow Articles by Suzuki, M.
Right arrow Articles by McCarty, D. R.

THE PLANT CELL, Vol 9, Issue 5 799-807, Copyright © 1997 by American Society of Plant Biologists

RESEARCH ARTICLE

The Conserved B3 Domain of VIVIPAROUS1 Has a Cooperative DNA Binding Activity

M. Suzuki, C. Y. Kao and D. R. McCarty
Program in Plant Molecular and Cellular Biology, Horticultural Sciences Department, University of Florida, Gainesville, Florida 32605

The biochemical activities that underlie the genetically defined activator and repressor functions of the VIVIPAROUS1 (VP1) protein have resisted in vitro analysis. Here, we show that a glutathione S-transferase (GST) fusion protein, including only the highly conserved B3 domain of VP1, has a highly cooperative, sequence-specific DNA binding activity. GST fusion proteins that include larger regions of the VP1 protein have very low activity, indicating that removal of the flanking protein sequences is necessary to elicit DNA binding in vitro. DNA competition and DNase I footprinting analyses show that B3 binds specifically to the Sph element involved in VP1 activation of the C1 gene, whereas binding to the G-box-type VP1-responsive element is of low affinity and is nonspecific. Footprint analysis of the C1 promoter revealed that sequences flanking the core TCCATGCAT motif of Sph also contribute to the recognition of the Sph element in its native context. The salient features of the in vitro GST-B3 DNA interaction are in good agreement with the protein and DNA sequence requirements defined by the functional analyses of VP1 and VP1-responsive elements in maize cells.


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
X. Tang, A. Hou, M. Babu, V. Nguyen, L. Hurtado, Q. Lu, J. C. Reyes, A. Wang, W. A. Keller, J. J. Harada, et al.
The Arabidopsis BRAHMA Chromatin-Remodeling ATPase Is Involved in Repression of Seed Maturation Genes in Leaves
Plant Physiology, July 1, 2008; 147(3): 1143 - 1157.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Suzuki, S. Latshaw, Y. Sato, A. M. Settles, K. E. Koch, L. C. Hannah, M. Kojima, H. Sakakibara, and D. R. McCarty
The Maize Viviparous8 Locus, Encoding a Putative ALTERED MERISTEM PROGRAM1-Like Peptidase, Regulates Abscisic Acid Accumulation and Coordinates Embryo and Endosperm Development
Plant Physiology, March 1, 2008; 146(3): 1193 - 1206.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Kobayashi, Y. Ogo, R. N. Itai, H. Nakanishi, M. Takahashi, S. Mori, and N. K. Nishizawa
The transcription factor IDEF1 regulates the response to and tolerance of iron deficiency in plants
PNAS, November 27, 2007; 104(48): 19150 - 19155.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Y. Yang, Y. Z. Ma, Z. S. Xu, X. M. Chen, Z. H. He, Z. Yu, M. Wilkinson, H. D. Jones, P. R. Shewry, and L. Q. Xia
Isolation and characterization of Viviparous-1 genes in wheat cultivars with distinct ABA sensitivity and pre-harvest sprouting tolerance
J. Exp. Bot., August 1, 2007; 58(11): 2863 - 2871.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
X. Niu, W. Zheng, B.-R. Lu, G. Ren, W. Huang, S. Wang, J. Liu, Z. Tang, D. Luo, Y. Wang, et al.
An Unusual Posttranscriptional Processing in Two Betaine Aldehyde Dehydrogenase Loci of Cereal Crops Directed by Short, Direct Repeats in Response to Stress Conditions
Plant Physiology, April 1, 2007; 143(4): 1929 - 1942.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Suzuki, H. H.-Y. Wang, and D. R. McCarty
Repression of the LEAFY COTYLEDON 1/B3 Regulatory Network in Plant Embryo Development by VP1/ABSCISIC ACID INSENSITIVE 3-LIKE B3 Genes
Plant Physiology, February 1, 2007; 143(2): 902 - 911.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
X. Cao, L. M. Costa, C. Biderre-Petit, B. Kbhaya, N. Dey, P. Perez, D. R. McCarty, J. F. Gutierrez-Marcos, and P. W. Becraft
Abscisic Acid and Stress Signals Induce Viviparous1 Expression in Seed and Vegetative Tissues of Maize
Plant Physiology, February 1, 2007; 143(2): 720 - 731.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Kotak, E. Vierling, H. Baumlein, and P. v. Koskull-Doring
A Novel Transcriptional Cascade Regulating Expression of Heat Stress Proteins during Seed Development of Arabidopsis
PLANT CELL, January 1, 2007; 19(1): 182 - 195.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Saez, N. Robert, M. H. Maktabi, J. I. Schroeder, R. Serrano, and P. L. Rodriguez
Enhancement of Abscisic Acid Sensitivity and Reduction of Water Consumption in Arabidopsis by Combined Inactivation of the Protein Phosphatases Type 2C ABI1 and HAB1
Plant Physiology, August 1, 2006; 141(4): 1389 - 1399.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
S. Konishi, T. Izawa, S. Y. Lin, K. Ebana, Y. Fukuta, T. Sasaki, and M. Yano
An SNP Caused Loss of Seed Shattering During Rice Domestication
Science, June 2, 2006; 312(5778): 1392 - 1396.
[Abstract] [Full Text] [PDF]

Home page
 Protein Sci.Home page
J. K. Waltner, F. C. Peterson, B. L. Lytle, and B. F. Volkman
Structure of the B3 domain from Arabidopsis thaliana protein At1g16640
Protein Sci., September 1, 2005; 14(9): 2478 - 2483.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S.-H. Shiu, M.-C. Shih, and W.-H. Li
Transcription Factor Families Have Much Higher Expansion Rates in Plants than in Animals
Plant Physiology, September 1, 2005; 139(1): 18 - 26.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Suzuki, M. G. Ketterling, and D. R. McCarty
Quantitative Statistical Analysis of cis-Regulatory Sequences in ABA/VP1- and CBF/DREB1-Regulated Genes of Arabidopsis
Plant Physiology, September 1, 2005; 139(1): 437 - 447.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
X. Zhang, V. Garreton, and N.-H. Chua
The AIP2 E3 ligase acts as a novel negative regulator of ABA signaling by promoting ABI3 degradation
Genes & Dev., July 1, 2005; 19(13): 1532 - 1543.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. Tsukagoshi, T. Saijo, D. Shibata, A. Morikami, and K. Nakamura
Analysis of a Sugar Response Mutant of Arabidopsis Identified a Novel B3 Domain Protein That Functions as an Active Transcriptional Repressor
Plant Physiology, June 1, 2005; 138(2): 675 - 685.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
F. Zhang and T. Peterson
Comparisons of Maize pericarp color1 Alleles Reveal Paralogous Gene Recombination and an Organ-Specific Enhancer Region
PLANT CELL, March 1, 2005; 17(3): 903 - 914.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Y. Kagaya, R. Toyoshima, R. Okuda, H. Usui, A. Yamamoto, and T. Hattori
LEAFY COTYLEDON1 Controls Seed Storage Protein Genes through Its Regulation of FUSCA3 and ABSCISIC ACID INSENSITIVE3
Plant Cell Physiol., March 1, 2005; 46(3): 399 - 406.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Y. Kagaya, R. Okuda, A. Ban, R. Toyoshima, K. Tsutsumida, H. Usui, A. Yamamoto, and T. Hattori
Indirect ABA-dependent Regulation of Seed Storage Protein Genes by FUSCA3 Transcription Factor in Arabidopsis
Plant Cell Physiol., February 1, 2005; 46(2): 300 - 311.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Z. Xie, Z.-L. Zhang, X. Zou, J. Huang, P. Ruas, D. Thompson, and Q. J. Shen
Annotations and Functional Analyses of the Rice WRKY Gene Superfamily Reveal Positive and Negative Regulators of Abscisic Acid Signaling in Aleurone Cells
Plant Physiology, January 1, 2005; 137(1): 176 - 189.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Zou, J. R. Seemann, D. Neuman, and Q. J. Shen
A WRKY Gene from Creosote Bush Encodes an Activator of the Abscisic Acid Signaling Pathway
J. Biol. Chem., December 31, 2004; 279(53): 55770 - 55779.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. Yamasaki, T. Kigawa, M. Inoue, M. Tateno, T. Yamasaki, T. Yabuki, M. Aoki, E. Seki, T. Matsuda, Y. Tomo, et al.
Solution Structure of the B3 DNA Binding Domain of the Arabidopsis Cold-Responsive Transcription Factor RAV1
PLANT CELL, December 1, 2004; 16(12): 3448 - 3459.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
T. Kroj, G. Savino, C. Valon, J. Giraudat, and F. Parcy
Regulation of storage protein gene expression in Arabidopsis
Development, December 15, 2003; 130(24): 6065 - 6073.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. B. Chandrasekharan, G. Li, K. J. Bishop, and T. C. Hall
S Phase Progression Is Required for Transcriptional Activation of the {beta}-Phaseolin Promoter
J. Biol. Chem., November 14, 2003; 278(46): 45397 - 45405.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. Ogawa, A. Hanada, Y. Yamauchi, A. Kuwahara, Y. Kamiya, and S. Yamaguchi
Gibberellin Biosynthesis and Response during Arabidopsis Seed Germination
PLANT CELL, July 1, 2003; 15(7): 1591 - 1604.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Suzuki, M. G. Ketterling, Q.-B. Li, and D. R. McCarty
Viviparous1 Alters Global Gene Expression Patterns through Regulation of Abscisic Acid Signaling
Plant Physiology, July 1, 2003; 132(3): 1664 - 1677.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
D. Yamauchi
Regulation of Gene Expression of a Cysteine Proteinase, EP-C1, by a VIVIPAROUS1-like Factor from Common Bean
Plant Cell Physiol., June 15, 2003; 44(6): 649 - 652.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Lara, L. Onate-Sanchez, Z. Abraham, C. Ferrandiz, I. Diaz, P. Carbonero, and J. Vicente-Carbajosa
Synergistic Activation of Seed Storage Protein Gene Expression in Arabidopsis by ABI3 and Two bZIPs Related to OPAQUE2
J. Biol. Chem., May 30, 2003; 278(23): 21003 - 21011.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
I. M. Brocard-Gifford, T. J. Lynch, and R. R. Finkelstein
Regulatory Networks in Seeds Integrating Developmental, Abscisic Acid, Sugar, and Light Signaling
Plant Physiology, January 1, 2003; 131(1): 78 - 92.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. Kagaya, T. Hobo, M. Murata, A. Ban, and T. Hattori
Abscisic Acid-Induced Transcription Is Mediated by Phosphorylation of an Abscisic Acid Response Element Binding Factor, TRAB1
PLANT CELL, December 1, 2002; 14(12): 3177 - 3189.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. Ishizaki, Y. Shimizu-Ueda, S. Okada, M. Yamamoto, M. Fujisawa, K. T. Yamato, H. Fukuzawa, and K. Ohyama
Multicopy genes uniquely amplified in the Y chromosome-specific repeats of the liverwort Marchantia polymorpha
Nucleic Acids Res., November 1, 2002; 30(21): 4675 - 4681.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. S. McKibbin, M. D. Wilkinson, P. C. Bailey, J. E. Flintham, L. M. Andrew, P. A. Lazzeri, M. D. Gale, J. R. Lenton, and M. J. Holdsworth
Transcripts of Vp-1 homeologues are misspliced in modern wheat and ancestral species
PNAS, July 23, 2002; 99(15): 10203 - 10208.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
Y. Y. Levy, S. Mesnage, J. S. Mylne, A. R. Gendall, and C. Dean
Multiple Roles of Arabidopsis VRN1 in Vernalization and Flowering Time Control
Science, July 12, 2002; 297(5579): 243 - 246.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
E. Nambara, M. Suzuki, S. Abrams, D. R. McCarty, Y. Kamiya, and P. McCourt
A Screen for Genes That Function in Abscisic Acid Signaling in Arabidopsis thaliana
Genetics, July 1, 2002; 161(3): 1247 - 1255.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Bensmihen, S. Rippa, G. Lambert, D. Jublot, V. Pautot, F. Granier, J. Giraudat, and F. Parcy
The Homologous ABI5 and EEL Transcription Factors Function Antagonistically to Fine-Tune Gene Expression during Late Embryogenesis
PLANT CELL, June 1, 2002; 14(6): 1391 - 1403.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
R. R. Finkelstein, S. S. L. Gampala, and C. D. Rock
Abscisic Acid Signaling in Seeds and Seedlings
PLANT CELL, May 1, 2002; 14(90001): S15 - 45.
[Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Miyoshi, Y. Kagaya, Y. Ogawa, Y. Nagato, and T. Hattori
Temporal and Spatial Expression Pattern of the OSVP1 and OSEM Genes during Seed Development in Rice
Plant Cell Physiol., March 1, 2002; 43(3): 307 - 313.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. M. Franco-Zorrilla, P. Cubas, J. A. Jarillo, B. Fernandez-Calvin, J. Salinas, and J. M. Martinez-Zapater
AtREM1, a Member of a New Family of B3 Domain-Containing Genes, Is Preferentially Expressed in Reproductive Meristems
Plant Physiology, February 1, 2002; 128(2): 418 - 427.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. M. Mack and N. M. Crawford
The Arabidopsis TAG1 Transposase Has an N-Terminal Zinc Finger DNA Binding Domain That Recognizes Distinct Subterminal Motifs
PLANT CELL, October 1, 2001; 13(10): 2319 - 2331.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. L. Stone, L. W. Kwong, K. M. Yee, J. Pelletier, L. Lepiniec, R. L. Fischer, R. B. Goldberg, and J. J. Harada
LEAFY COTYLEDON2 encodes a B3 domain transcription factor that induces embryo development
PNAS, September 25, 2001; 98(20): 11806 - 11811.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
D. Yamauchi
A TGACGT Motif in the 5'-Upstream Region of {{alpha}}-Amylase Gene from Vigna mungo is a cis-Element for Expression in Cotyledons of Germinated Seeds
Plant Cell Physiol., June 1, 2001; 42(6): 635 - 641.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
C. Spelt, F. Quattrocchio, J. N. M. Mol, and R. Koes
anthocyanin1 of Petunia Encodes a Basic Helix-Loop-Helix Protein That Directly Activates Transcription of Structural Anthocyanin Genes
PLANT CELL, September 1, 2000; 12(9): 1619 - 1632.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
D. Hagenbeek, R. S. Quatrano, and C. D. Rock
Trivalent Ions Activate Abscisic Acid-Inducible Promoters through an ABI1-Dependent Pathway in Rice Protoplasts
Plant Physiology, August 1, 2000; 123(4): 1553 - 1560.
[Abstract] [Full Text]

Home page
 Plant CellHome page
A. Rohde, R. De Rycke, T. Beeckman, G. Engler, M. Van Montagu, and W. Boerjan
ABI3 Affects Plastid Differentiation in Dark-Grown Arabidopsis Seedlings
PLANT CELL, January 1, 2000; 12(1): 35 - 52.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Hobo, Y. Kowyama, and T. Hattori
A bZIP factor, TRAB1, interacts with VP1 and mediates abscisic acid-induced transcription
PNAS, December 21, 1999; 96(26): 15348 - 15353.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Ulmasov, G. Hagen, and T. J. Guilfoyle
Activation and repression of transcription by auxin-response factors
PNAS, May 11, 1999; 96(10): 5844 - 5849.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. B. Singh
Transcriptional Regulation in Plants: The Importance of Combinatorial Control
Plant Physiology, December 1, 1998; 118(4): 1111 - 1120.
[Full Text]

Home page
 Plant Physiol.Home page
T. Guilfoyle, G. Hagen, T. Ulmasov, and J. Murfett
How Does Auxin Turn On Genes?
Plant Physiology, October 1, 1998; 118(2): 341 - 347.
[Full Text]

Home page
 GeneticsHome page
C. M. Steber, S. E. Cooney, and P. McCourt
Isolation of the GA-Response Mutant sly1 as a Suppressor of ABI1-1 in Arabidopsis thaliana
Genetics, June 1, 1998; 149(2): 509 - 521.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
T. F. Schultz, J. Medina, A. Hill, and R. S. Quatrano
14-3-3 Proteins Are Part of an Abscisic Acid–VIVIPAROUS1 (VP1) Response Complex in the Em Promoter and Interact with VP1 and EmBP1
PLANT CELL, May 1, 1998; 10(5): 837 - 848.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. S. L. Gampala, D. Hagenbeek, and C. D. Rock
Functional Interactions of Lanthanum and Phospholipase D with the Abscisic Acid Signaling Effectors VP1 and ABI1-1 in Rice Protoplasts
J. Biol. Chem., March 23, 2001; 276(13): 9855 - 9860.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. S. L. Gampala, R. R. Finkelstein, S. S. M. Sun, and C. D. Rock
ABI5 Interacts with Abscisic Acid Signaling Effectors in Rice Protoplasts
J. Biol. Chem., January 11, 2002; 277(3): 1689 - 1694.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1997 by the American Society of Plant Biologists