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THE PLANT CELL, Vol 9, Issue 6 869-878, Copyright © 1997 by American Society of Plant Biologists
Activation of the Silent psbA1 Gene in the Cyanobacterium Synechocystis sp Strain 6803 Produces a Novel and Functional D1 Protein
G. F. Salih and C. Jansson
Department of Biochemistry, The Arrhenius Laboratories, Stockholm University, S-106 91 Stockholm, Sweden
The photosystem II reaction center protein D1 in Synechocystis sp strain
6803 is encoded by the psbA2 and psbA3 genes of the three-membered psbA
gene family. The silent and divergent psbA1 copy of the psbA gene family
was activated by exchanging part of its upstream region with a
corresponding fragment of the psbA2 copy. The light-regulated expression of
the activated psbA1 gene showed that the inserted psbA2 segment contains
the information necessary for light-dependent as well as
high-light-stimulated transcription. The activated psbA1 gene expressed a
novel D1 protein, D1[prime]. A mutant strain containing psbA1 as the only
active psbA gene grew photoautotrophically at a rate comparable to that of
the wild type. This finding demonstrates that despite its unusual amino
acid sequence, D1[prime] is exchangeable for D1 in the photosystem II
complex, at least under normal laboratory conditions. The D1[prime] protein
was found to have a degradation rate similar to that of the D1 protein
under low- or high-light conditions. Another mutant containing the
activated psbA1 gene together with the psbA2 and psbA3 genes produced both
the D1 and D1[prime] proteins.
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