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THE PLANT CELL, Vol 9, Issue 6 967-978, Copyright © 1997 by American Society of Plant Biologists
Retrotransposon Insertion into the Maize waxy Gene Results in Tissue-Specific RNA Processing
S. Marillonnet and S. R. Wessler
Departments of Botany and Genetics, University of Georgia, Athens, Georgia 30602
We previously reported that three alleles of the maize waxy (wx) gene were
alternatively spliced as a result of the insertion of retrotransposons into
intronic sequences. In addition, inefficient splicing of element sequences
with the surrounding intron produced wild-type transcripts that presumably
were responsible for the observed residual gene expression in the
endosperm. In this study, we report that one of these alleles, wxG, has a
tissue-specific phenotype with 30-fold more WX enzymatic activity in pollen
than in the endosperm. Quantification of wxG-encoded transcripts in pollen
and the endosperm demonstrates that this difference can be accounted for by
tissue-specific differences in RNA processing. Specifically, there is
~30-fold more correctly spliced RNA in pollen than in the endosperm. Based
on an analogy to similar examples of tissue-specific alternative splicing
in animal systems, we hypothesize that the tissue-specific phenotype of the
wxG allele may reflect differences in the concentration of splicing factors
in these tissues.
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