|
THE PLANT CELL, Vol 9, Issue 8 1369-1380, Copyright © 1997 by American Society of Plant Biologists
Chlamydomonas Xanthophyll Cycle Mutants Identified by Video Imaging of Chlorophyll Fluorescence Quenching
K. K. Niyogi, O. Bjorkman and A. R. Grossman
Carnegie Institution of Washington, Department of Plant Biology, 290 Panama Street, Stanford, California 94305
The photosynthetic apparatus in plants is protected against oxidative
damage by processes that dissipate excess absorbed light energy as heat
within the light-harvesting complexes. This dissipation of excitation
energy is measured as nonphotochemical quenching of chlorophyll
fluorescence. Nonphotochemical quenching depends primarily on the [delta]pH
that is generated by photosynthetic electron transport, and it is also
correlated with the amounts of zeaxanthin and antheraxanthin that are
formed from violaxanthin by the operation of the xanthophyll cycle. To
perform a genetic dissection of nonphotochemical quenching, we have
isolated npq mutants of Chlamydomonas by using a digital video-imaging
system. In excessive light, the npq1 mutant is unable to convert
violaxanthin to antheraxanthin and zeaxanthin; this reaction is catalyzed
by violaxanthin de-epoxidase. The npq2 mutant appears to be defective in
zeaxanthin epoxidase activity, because it accumulates zeaxanthin and
completely lacks antheraxanthin and violaxanthin under all light
conditions. Characterization of these mutants demonstrates that a component
of nonphotochemical quenching that develops in vivo in Chlamydomonas
depends on the accumulation of zeaxanthin and antheraxanthin via the
xanthophyll cycle. However, observation of substantial, rapid,
[delta]pH-dependent nonphotochemical quenching in the npq1 mutant
demonstrates that the formation of zeaxanthin and antheraxanthin via
violaxanthin de-epoxidase activity is not required for all
[delta]pH-dependent nonphotochemical quenching in this alga. Furthermore,
the xanthophyll cycle is not required for survival of Chlamydomonas in
excessive light.
This article has been cited by other articles:
|
|
|
|
 
X. Zhang, B. Wollenweber, D. Jiang, F. Liu, and J. Zhao
Water deficits and heat shock effects on photosynthesis of a transgenic Arabidopsis thaliana constitutively expressing ABP9, a bZIP transcription factor
J. Exp. Bot.,
March 1, 2008;
59(4):
839 - 848.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Lenk, L. Chaerle, E. E. Pfundel, G. Langsdorf, D. Hagenbeek, H. K. Lichtenthaler, D. Van Der Straeten, and C. Buschmann
Multispectral fluorescence and reflectance imaging at the leaf level and its possible applications
J. Exp. Bot.,
March 1, 2007;
58(4):
807 - 814.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Ozaki, M. Ikeuchi, T. Ogawa, H. Fukuzawa, and K. Sonoike
Large-Scale Analysis of Chlorophyll Fluorescence Kinetics in Synechocystis sp. PCC 6803: Identification of the Factors Involved in the Modulation of Photosystem Stoichiometry
Plant Cell Physiol.,
March 1, 2007;
48(3):
451 - 458.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Steinbrenner and G. Sandmann
Transformation of the Green Alga Haematococcus pluvialis with a Phytoene Desaturase for Accelerated Astaxanthin Biosynthesis
Appl. Envir. Microbiol.,
December 1, 2006;
72(12):
7477 - 7484.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Kovacs, J. Damkjaer, S. Kereiche, C. Ilioaia, A. V. Ruban, E. J. Boekema, S. Jansson, and P. Horton
Lack of the Light-Harvesting Complex CP24 Affects the Structure and Function of the Grana Membranes of Higher Plant Chloroplasts
PLANT CELL,
November 1, 2006;
18(11):
3106 - 3120.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Peng, J. Ma, W. Chi, J. Guo, S. Zhu, Q. Lu, C. Lu, and L. Zhang
LOW PSII ACCUMULATION1 Is Involved in Efficient Assembly of Photosystem II in Arabidopsis thaliana
PLANT CELL,
April 1, 2006;
18(4):
955 - 969.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. M. Dent, C. M. Haglund, B. L. Chin, M. C. Kobayashi, and K. K. Niyogi
Functional Genomics of Eukaryotic Photosynthesis Using Insertional Mutagenesis of Chlamydomonas reinhardtii
Plant Physiology,
February 1, 2005;
137(2):
545 - 556.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. Rymarquis, J. M. Handley, M. Thomas, and D. B. Stern
Beyond Complementation. Map-Based Cloning in Chlamydomonas reinhardtii
Plant Physiology,
February 1, 2005;
137(2):
557 - 566.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Horton and A. Ruban
Molecular design of the photosystem II light-harvesting antenna: photosynthesis and photoprotection
J. Exp. Bot.,
January 1, 2005;
56(411):
365 - 373.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. K. Niyogi, X.-P. Li, V. Rosenberg, and H.-S. Jung
Is PsbS the site of non-photochemical quenching in photosynthesis?
J. Exp. Bot.,
January 1, 2005;
56(411):
375 - 382.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Nield, K. Redding, and M. Hippler
Remodeling of Light-Harvesting Protein Complexes in Chlamydomonas in Response to Environmental Changes
Eukaryot. Cell,
December 1, 2004;
3(6):
1370 - 1380.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. C. Posewitz, S. L. Smolinski, S. Kanakagiri, A. Melis, M. Seibert, and M. L. Ghirardi
Hydrogen Photoproduction Is Attenuated by Disruption of an Isoamylase Gene in Chlamydomonas reinhardtii
PLANT CELL,
August 1, 2004;
16(8):
2151 - 2163.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-D. Rochaix
Genetics of the Biogenesis and Dynamics of the Photosynthetic Machinery in Eukaryotes
PLANT CELL,
July 1, 2004;
16(7):
1650 - 1660.
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Oxborough
Imaging of chlorophyll a fluorescence: theoretical and practical aspects of an emerging technique for the monitoring of photosynthetic performance
J. Exp. Bot.,
June 1, 2004;
55(400):
1195 - 1205.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. L. Gutman and K. K. Niyogi
Chlamydomonas and Arabidopsis. A Dynamic Duo
Plant Physiology,
June 1, 2004;
135(2):
607 - 610.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Baroli, B. L. Gutman, H. K. Ledford, J. W. Shin, B. L. Chin, M. Havaux, and K. K. Niyogi
Photo-oxidative Stress in a Xanthophyll-deficient Mutant of Chlamydomonas
J. Biol. Chem.,
February 20, 2004;
279(8):
6337 - 6344.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. D. Bosco, L. Lezhneva, A. Biehl, D. Leister, H. Strotmann, G. Wanner, and J. Meurer
Inactivation of the Chloroplast ATP Synthase {gamma} Subunit Results in High Non-photochemical Fluorescence Quenching and Altered Nuclear Gene Expression in Arabidopsis thaliana
J. Biol. Chem.,
January 9, 2004;
279(2):
1060 - 1069.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Jin, K. Yokthongwattana, J. E.W. Polle, and A. Melis
Role of the Reversible Xanthophyll Cycle in the Photosystem II Damage and Repair Cycle in Dunaliella salina
Plant Physiology,
May 1, 2003;
132(1):
352 - 364.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Baroli, A. D. Do, T. Yamane, and K. K. Niyogi
Zeaxanthin Accumulation in the Absence of a Functional Xanthophyll Cycle Protects Chlamydomonas reinhardtii from Photooxidative Stress
PLANT CELL,
April 1, 2003;
15(4):
992 - 1008.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. S. Miller, E. Kutter, G. Mosig, F. Arisaka, T. Kunisawa, and W. Ruger
Bacteriophage T4 Genome
Microbiol. Mol. Biol. Rev.,
March 1, 2003;
67(1):
86 - 156.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. G. Walters, F. Shephard, J. J.M. Rogers, S. A. Rolfe, and P. Horton
Identification of Mutants of Arabidopsis Defective in Acclimation of Photosynthesis to the Light Environment
Plant Physiology,
February 1, 2003;
131(2):
472 - 481.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Kanazawa and D. M. Kramer
In vivo modulation of nonphotochemical exciton quenching (NPQ) by regulation of the chloroplast ATP synthase
PNAS,
October 1, 2002;
99(20):
12789 - 12794.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X.-P. Li, A. M. Gilmore, and K. K. Niyogi
Molecular and Global Time-resolved Analysis of a psbS Gene Dosage Effect on pH- and Xanthophyll Cycle-dependent Nonphotochemical Quenching in Photosystem II
J. Biol. Chem.,
September 6, 2002;
277(37):
33590 - 33597.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Govindjee
A Role for a Light-Harvesting Antenna Complex of Photosystem II in Photoprotection
PLANT CELL,
August 1, 2002;
14(8):
1663 - 1668.
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Elrad, K. K. Niyogi, and A. R. Grossman
A Major Light-Harvesting Polypeptide of Photosystem II Functions in Thermal Dissipation
PLANT CELL,
August 1, 2002;
14(8):
1801 - 1816.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-D. Rochaix
Assembly, Function, and Dynamics of the Photosynthetic Machinery in Chlamydomonas reinhardtii
Plant Physiology,
December 1, 2001;
127(4):
1394 - 1398.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. E. W. Polle, K. K. Niyogi, and A. Melis
Absence of Lutein, Violaxanthin and Neoxanthin Affects the Functional Chlorophyll Antenna Size of Photosystem-II but not that of Photosystem-I in the Green Alga Chlamydomonas reinhardtii
Plant Cell Physiol.,
May 1, 2001;
42(5):
482 - 491.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Majeran, J. Olive, D. Drapier, O. Vallon, and F.-A. Wollman
The Light Sensitivity of ATP Synthase Mutants of Chlamydomonas reinhardtii
Plant Physiology,
May 1, 2001;
126(1):
421 - 433.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
P. Müller, X.-P. Li, and K. K. Niyogi
Non-Photochemical Quenching. A Response to Excess Light Energy
Plant Physiology,
April 1, 2001;
125(4):
1558 - 1566.
[Full Text]
|
|
|
|
|
|
|
M. Havaux and K. K. Niyogi
The violaxanthin cycle protects plants from photooxidative damage by more than one mechanism
PNAS,
July 20, 1999;
96(15):
8762 - 8767.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. V. Ruban and P. Horton
The Xanthophyll Cycle Modulates the Kinetics of Nonphotochemical Energy Dissipation in Isolated Light-Harvesting Complexes, Intact Chloroplasts, and Leaves of Spinach
Plant Physiology,
February 1, 1999;
119(2):
531 - 542.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
B. J. Pogson, K. K. Niyogi, O. Bjorkman, and D. DellaPenna
Altered xanthophyll compositions adversely affect chlorophyll accumulation and nonphotochemical quenching in Arabidopsis mutants
PNAS,
October 27, 1998;
95(22):
13324 - 13329.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. K. Niyogi, A. R. Grossman, and O. Björkman
Arabidopsis Mutants Define a Central Role for the Xanthophyll Cycle in the Regulation of Photosynthetic Energy Conversion
PLANT CELL,
July 1, 1998;
10(7):
1121 - 1134.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
D. D. Wykoff, J. P. Davies, A. Melis, and A. R. Grossman
The Regulation of Photosynthetic Electron Transport during Nutrient Deprivation in Chlamydomonas reinhardtii
Plant Physiology,
May 1, 1998;
117(1):
129 - 139.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
K. K. Niyogi, O. Bjorkman, and A. R. Grossman
The roles of specific xanthophylls in photoprotection
PNAS,
December 9, 1997;
94(25):
14162 - 14167.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
