|
THE PLANT CELL, Vol 9, Issue 8 1495-1504, Copyright © 1997 by American Society of Plant Biologists
A Transcriptionally Active State Is Required for Post-Transcriptional Silencing (Cosuppression) of Nitrate Reductase Host Genes and Transgenes
H. Vaucheret, L. Nussaume, J. C. Palauqui, I. Quillere and T. Elmayan
Laboratoire de Biologie Cellulaire, Centre INRA de Versailles, F-78026 Versailles Cedex, France
Using tobacco nitrate reductase cosuppression as a model system of
post-transcriptional gene silencing, we analyzed the influence of DNA and
RNA dosages both together and independently. For this purpose, zero, one,
two, or four active or transcriptionally silenced copies of a cauliflower
mosaic virus 35S-Nia2 transgene were combined by transformation and
subsequent crosses with zero, one, two, three, or four active, disrupted,
or transcriptionally repressed copies of the wild-type host Nia genes. The
analysis of the corresponding transgenic lines revealed that (1) the
percentage of isogenic plants that are affected by cosuppression depends
directly upon the relative dosage of both host gene and transgene; (2)
transcriptional silencing of the 35S-Nia transgene impedes cosuppression;
and (3) the absence of host gene transcription reduces the frequency of
cosuppression or delays its triggering. Taken together, these results
indicate that transgene DNA per se is not sufficient to trigger
post-transcriptional cosuppression of nitrate reductase host genes and
transgenes. The requirement for a transcriptionally active state is
discussed with respect to both the RNA dosage and the DNA-DNA pairing
hypotheses.
This article has been cited by other articles:
|
|
|
|
 
A. Kanazawa, M. O'Dell, and R. P. Hellens
Epigenetic Inactivation of Chalcone Synthase-A Transgene Transcription in Petunia Leads to a Reversion of the Post-Transcriptional Gene Silencing Phenotype
Plant Cell Physiol.,
April 1, 2007;
48(4):
638 - 647.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Chen, X. Zhao, Z. Shao, Z. Wei, Y. Wang, L. Zhu, J. Zhao, M. Sun, R. He, and G. He
Rice UDP-Glucose Pyrophosphorylase1 Is Essential for Pollen Callose Deposition and Its Cosuppression Results in a New Type of Thermosensitive Genic Male Sterility
PLANT CELL,
March 1, 2007;
19(3):
847 - 861.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Fojtova, A. Bleys, J. Bedrichova, H. Van Houdt, K. Krizova, A. Depicker, and A. Kovarik
The trans-silencing capacity of invertedly repeated transgenes depends on their epigenetic state in tobacco.
Nucleic Acids Res.,
January 1, 2006;
34(8):
2280 - 2293.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Q. Wang and G. G. Carmichael
Effects of Length and Location on the Cellular Response to Double-Stranded RNA
Microbiol. Mol. Biol. Rev.,
September 1, 2004;
68(3):
432 - 452.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Vaucheret, C. Beclin, and M. Fagard
Post-transcriptional gene silencing in plants
J. Cell Sci.,
January 9, 2001;
114(17):
3083 - 3091.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Yamaguchi and M. Nishimura
Reduction to below Threshold Levels of Glycolate Oxidase Activities in Transgenic Tobacco Enhances Photoinhibition during Irradiation
Plant Cell Physiol.,
December 1, 2000;
41(12):
1397 - 1406.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. D. Day, E. Lee, J. Kobayashi, L. D. Holappa, H. Albert, and D. W. Ow
Transgene integration into the same chromosome location can produce alleles that express at a predictable level, or alleles that are differentially silenced
Genes & Dev.,
November 15, 2000;
14(22):
2869 - 2880.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
F. G. Ratcliff, S. A. MacFarlane, and D. C. Baulcombe
Gene Silencing without DNA: RNA-Mediated Cross-Protection between Viruses
PLANT CELL,
July 1, 1999;
11(7):
1207 - 1216.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
H. Holtorf, H. Schob, C. Kunz, R. Waldvogel, and F. Meins , Jr.
Stochastic and Nonstochastic Post-Transcriptional Silencing of Chitinase and ß-1,3-Glucanase Genes Involves Increased RNA Turnover—Possible Role for Ribosome-Independent RNA Degradation
PLANT CELL,
March 1, 1999;
11(3):
471 - 484.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. B. Bahramian and H. Zarbl
Transcriptional and Posttranscriptional Silencing of Rodent alpha 1(I) Collagen by a Homologous Transcriptionally Self-Silenced Transgene
Mol. Cell. Biol.,
January 1, 1999;
19(1):
274 - 283.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Schiebel, T. Pélissier, L. Riedel, S. Thalmeir, R. Schiebel, D. Kempe, F. Lottspeich, H. L. Sänger, and M. Wassenegger
Isolation of an RNA-Directed RNA Polymerase –Specific cDNA Clone from Tomato
PLANT CELL,
December 1, 1998;
10(12):
2087 - 2102.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. Stam, A. Viterbo, J. N. M. Mol, and J. M. Kooter
Position-Dependent Methylation and Transcriptional Silencing of Transgenes in Inverted T-DNA Repeats: Implications for Posttranscriptional Silencing of Homologous Host Genes in Plants
Mol. Cell. Biol.,
November 1, 1998;
18(11):
6165 - 6177.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. Elmayan, S. Balzergue, F. Béon, V. Bourdon, J. Daubremet, Y. Guénet, P. Mourrain, J.-C. Palauqui, S. Vernhettes, T. Vialle, et al.
Arabidopsis Mutants Impaired in Cosuppression
PLANT CELL,
October 1, 1998;
10(10):
1747 - 1758.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J.-C. Palauqui and H. Vaucheret
Transgenes are dispensable for the RNA degradation step of cosuppression
PNAS,
August 4, 1998;
95(16):
9675 - 9680.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
