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THE PLANT CELL, Vol 9, Issue 9 1515-1525, Copyright © 1997 by American Society of Plant Biologists
Expression of the C4 Me1 Gene from Flaveria bidentis Requires an Interaction between 5[prime] and 3[prime] Sequences
J. S. Marshall, J. D. Stubbs, J. A. Chitty, B. Surin and W. C. Taylor
CSIRO Plant Industry, GPO Box 1600, Canberra 2601, Australia
The efficient functioning of C4 photosynthesis requires the strict
compartmentation of a suite of enzymes in either mesophyll or bundle sheath
cells. To determine the mechanism controlling bundle sheath cell-specific
expression of the NADP-malic enzyme, we made a set of chimeric constructs
using the 5[prime] and 3[prime] regions of the Flaveria bidentis Me1 gene
fused to the [beta]-glucuronidase gusA reporter gene. The pattern of GUS
activity in stably transformed F. bidentis plants was analyzed by
histochemical and cell separation techniques. We conclude that the 5[prime]
region of Me1 determines bundle sheath specificity, whereas the 3[prime]
region contains an apparent enhancer-like element that confers high-level
expression in leaves. The interaction of 5[prime] and 3[prime] sequences
was dependent on factors that are present in the C4 plant but not found in
tobacco.
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