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THE PLANT CELL, Vol 9, Issue 9 1647-1659, Copyright © 1997 by American Society of Plant Biologists
Inhibition of Pollen Tube Elongation by Microinjected Anti-Rop1Ps Antibodies Suggests a Crucial Role for Rho-Type GTPases in the Control of Tip Growth
Y. Lin and Z. Yang
Department of Plant Biology and Plant Biotechnology Center, Ohio State University, Columbus, Ohio 43210
Microinjection of anti-Rop1Ps antibodies was used to assess the function of
a tip-localized Rho-type GTPase, Rop, in controlling pollen tube growth.
Injected antibodies induced sustained growth arrest within 1 to 2 min after
injection but did not affect cytoplasmic streaming. Coinjection with Rop
rescued antibody-induced growth inhibition, indicating that injected
antibodies specifically block the activity of Rop GTPases. Antibody-induced
inhibition was significantly enhanced in the presence of a lower threshold
of extracellular [Ca2+] or a subinhibitory dosage of caffeine. In contrast,
injection of the C3 toxin, which inactivates a different Rho-type GTPase,
arrested tube elongation 10 to 20 min after injection. C3-induced growth
arrest was accompanied by the cessation of cytoplasmic streaming. These
data suggest that Rho-type GTPases play a pivotal role in the control of
pollen tube elongation. We propose that Rop may regulate a Ca2+-dependent
pathway involved in vesicle docking/fusion, whereas a C3-sensitive Rho
GTPase may mediate cytoplasmic streaming.
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