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THE PLANT CELL, Vol 9, Issue 9 1683-1696, Copyright © 1997 by American Society of Plant Biologists
Coexpression of the Maize [delta]-Zein and [beta]-Zein Genes Results in Stable Accumulation of [delta]-Zein in Endoplasmic Reticulum[mdash]Derived Protein Bodies Formed by [beta]-Zein
S. Bagga, H. P. Adams, F. D. Rodriguez, J. D. Kemp and C. Sengupta-Gopalan
Agronomy and Horticulture Department, New Mexico State University, Las Cruces, New Mexico 88003
Zeins, the major seed storage proteins of maize, are of four distinct
types: [alpha], [beta], [delta], and [gamma]. They are synthesized on the
rough endoplasmic reticulum (ER) in a sequential manner and deposited in
ER-derived protein bodies. We investigated the potential for producing
sulfur-rich [beta]-zein and [delta]-zein proteins in leaf and seed tissues
by expressing the corresponding genes in a constitutive manner in
transgenic tobacco. The [delta]-zein and [beta]-zein, when synthesized
individually, were stable in the vegetative tissues and were deposited in
unique, zein-specific ER-derived protein bodies. Coexpression of
[delta]-zein and [beta]-zein genes, however, showed that [delta]-zein was
colocalized in [beta]-zein-containing protein bodies and that the level of
[delta]-zein was fivefold higher in [delta]-/[beta]-zein plants than in
[delta]-zein plants. We conclude that [delta]-zein interacts with
[beta]-zein and that the interaction has a stabilizing effect on
[delta]-zein.
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