Plant Cell, Vol. 11, 2059-2060, October 1999, Copyright © 1999, American Society of Plant Physiologists
Correction
D. Shelley Lupold, Angelina G.F.S. Caoile, and David B. Stern (1999). Polyadenylation Occurs at Multiple Sites in Maize Mitochondrial cox2 mRNA and Is Independent of Editing Status. Plant Cell 11, 1565–1577.
Errors have been discovered in the formatting of Figure 3 on page 1569 and Figure 6 on page 1572. The corrected figures and their legends are given below.
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Figure 3.
Major 3' Ends and Polyadenylation Sites in the cox2 3' UTR.
(A) Determination of the mature 3' end of cox2 transcripts by S1 nuclease protection. The probe used was the 3' end-labeled SphI-NdeI restriction fragment of N6SB (see Figure 1A). The amount of probe used (1x or 2.5x) and the addition (+) or not (-) of mtRNA, yeast tRNA, and/or S1 nuclease are indicated. Size markers were HinfI-digested  X174 and an unrelated sequence ladder (data not shown). The protected fragments map from 360 to 365 nucleotides (nt) downstream of the stop codon. The self-annealed double-stranded probe is 270 bp.
(B) Sequences surrounding sites 7 and 20 in the cox2 3' UTR. The heterogeneous 3' ends are marked by arrows above the sequences, and poly(A) addition sites are marked by asterisks below the sequences, with the number indicating the number of cDNAs recovered for each site.
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Figure 6.
Editing Status of the Polyadenylated cDNAs.
(A) Filled circles represent sites that were edited in the sequenced clones, and open circles represent unedited sites. The number of polyadenylated clones having each pattern is indicated at left. Numbers at top represent editing sites.
(B) Editing status of bulk mtRNA. The sequencing gels show heterogeneity at each editing site; these sites are indicated by numbered asterisks, and the corresponding sequences and codons are indicated below the gels.
(C) Editing frequency of bulk RNA was determined by using a PhosphorImager to quantify the edited (lanes T) and unedited (lanes C) bands in (B) for each edited position, using nearby unedited T and C bands as controls for variation in band intensity related to sequence length and composition. The values for bulk RNA are averages of three separate experiments. pA, poly(A).
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