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The Plant Cell, Vol. 14, S5-S8, May 2002, Copyright © 2002,
American Society of Plant Biologists

INSIGHT

A Breakdown in Defense Signaling

Peter N. Doddsa and Claus Schwechheimerb

a Commonwealth Scientific and Industrial Research Organization Plant Industry Canberra, ACT 2601, Australia
b Center for Plant Molecular Biology Tübingen University 72076 Tübingen, Germany

One of the major mechanisms that plants use to escape disease involves a pathogen recognition system known as gene-for-gene resistance. This is controlled genetically by resistance (R) genes in plants, which encode proteins that recognize avirulence (Avr) products of an invading pathogen. Pathogen recognition triggers a wide range of both local and systemic responses whose cumulative effect is the inhibition of pathogen growth.

In the past decade, many R genes have been isolated that confer pathogen resistance to various plant species against a wide range of pathogens; however, the signaling processes that lead from initial pathogen recognition to the induction of the resistance response remain poorly understood (Glazebrook, 2001Go). Five exciting new articles address two downstream components of resistance signaling, RAR1 and SGT1 (Austin et al., 2002Go; Azevedo et al., 2002Go; Muskett et al., 2002Go, Tör et al., 2002Go; Tornero et al., 2002Go). Together, these articles provide critical evidence that protein degradation may be of pivotal importance for efficient pathogen responses and form the basis of a new paradigm for resistance signaling.

The majority of R genes identified encode intracellular proteins containing a predicted nucleotide binding site (NBS) followed by a series of leucine-rich repeats (LRR) at their C termini. In addition, NBS-LRR resistance proteins generally contain one of two types of N-terminal domains. These are either a domain that has homology with the Toll and Interleukin-1 Receptor proteins (TIR) or a predicted coiled-coil domain (CC). Functional studies of resistance proteins have indicated that the highly variable LRR domains determine recognition of the pathogen Avr product (Ellis et al., 1999Go; Jia et al., 2000Go; Dodds et al., 2001Go), whereas the more conserved TIR-NBS or CC-NBS regions are believed to propagate the perceived signal (Tao et al., 2000Go).

Two distinct resistance-signaling pathways have been described genetically, and at present, the R genes that are known to use each of these pathways correlate with the TIR and CC classes. The Arabidopsis genes EDS1 and PAD4 encode lipase-like proteins that interact with each other and mediate the downstream signaling of known TIR-type but not CC-type receptors (Feys et al., 2001Go). Conversely, the presumed membrane-bound protein encoded by NDR1 is required for many CC-type resistance proteins but does not affect resistance triggered by known TIR-type resistance proteins (Century et al., 1997Go; Aarts et al., 1998Go). In addition, at least two CC-type resistance genes, RPP8 and RPP13, function independently of EDS1 and NDR1, suggesting the existence of at least one additional signaling pathway (McDowell et al., 2000Go; Bittner-Eddy and Beynon, 2001Go). At some point, these distinct pathways presumably converge because they give rise to similar resistance responses.

The simple picture of independent linear pathways has now been complicated by the introduction of RAR1 and SGT1, which are required for signaling by sets of resistance genes that overlap the boundaries between the pathways defined previously (Table 1). The barley RAR1 gene (HvRAR1) is required for powdery mildew resistance controlled by many but not all of the genes at the Mla resistance locus and at some other R loci in barley (Jørgensen, 1996Go). HvRAR1 was isolated by map-based cloning and was found to encode a protein of unknown function that is conserved in all eukaryotes except yeast (Shirasu et al., 1999Go). Interestingly, initial clues regarding the function of the HvRAR1 protein came from the observation that animal RAR1 orthologs contain an additional domain with homology to the Saccharomyces cerevisiae protein SGT1 that is absent from plant RAR1 orthologs.


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  		Table 1. Summary of the Experimentally Proven Requirements for RAR1 and SGT1 for Specific R Gene–Mediated Pathways

 
SGT1 was identified initially as a genetic suppressor of yeast skp1 mutants (Kitagawa et al., 1999Go). The SKP1 protein is an evolutionarily conserved subunit of SCF-type E3 ubiquitin ligases that mediate the ubiquitylation of proteins that are targeted for degradation by the 26S proteasome (Deshaies, 1999Go; Bachmair et al., 2001Go). Biochemical analysis of yeast SGT1 indicated that it is a positive regulator of E3 ubiquitin ligase activity (Kitagawa et al., 1999Go). SGT1 homologs from Arabidopsis and barley have now been found to interact with RAR1 in the yeast two-hybrid system (Azevedo et al., 2002Go). In transiently transformed barley cells, double-stranded RNA inhibition of RAR1 or SGT1 resulted in the loss of Mla6-mediated resistance but did not affect the closely related Mla1 resistance gene, consistent with previous analysis of rar1 mutants (Azevedo et al., 2002Go). This suggested that SGT1 and RAR1 act together in mediating the Mla6-induced pathogen response.

Both SGT1 and RAR1 have been implicated more generally in defense signaling by the isolation of loss-of function mutants in Arabidopsis. These mutations have been isolated independently by several groups using genetic screens for loss of resistance mediated by different R genes (Austin et al., 2002Go; Muskett et al., 2002Go; Tör et al., 2002Go; Tornero et al., 2002Go). In contrast to barley, which contains only one SGT1 gene, the Arabidopsis genome contains two SGT1 orthologs, designated SGT1a and SGT1b, that seem to have arisen through a recent gene duplication (Austin et al., 2002Go). Although mutations in SGT1b were identified in these loss-of-resistance screens, no mutations were found in SGT1a (Austin et al., 2002Go; Muskett et al., 2002Go). Interestingly, the spectra of R genes that require RAR1 or SGT1 do not coincide with those of genes that use EDS1/PAD4 or NDR1, nor do they match the TIR-NBS or CC-NBS classifications (Table 1). In fact, R genes from both classes and both pathways may require RAR1 and/or SGT1b to varying degrees, so that some R genes require RAR1 and SGT1b, some require RAR1 or SGT1b only, and some require neither.

At this time, no known CC-NBS–type R gene has been shown to require SGT1b function. It could be hypothesized that the sample of tested R genes is too small to have led to the identification of an SGT1b-dependent CC-NBS–type R gene or that the yet uncharacterized SGT1a is specific for these R genes. Together, the genetic data suggest that RAR1- and/or SGT1b-mediated protein degradation may be involved in the responses that are controlled by some but not all R genes, that RAR1 function is dispensable for some SGT1b-mediated pathogen responses, and that SGT1b function is dispensable for some RAR1-mediated pathogen responses (Figure 1) . It also needs to be noted that the requirement for RAR1 and SGT1b is not always absolute. The responses mediated by some R genes are affected only modestly by the rar1 or sgt1b mutations, whereas others are inhibited completely.



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  		Figure 1. Differential Requirement of RAR1 and SGT1b as Downstream Components of Various R Protein Pathways.

The COP9 signalosome copurifies with RAR1 and SGT1b, but the anticipated requirement for this interaction in pathogen response needs to be verified experimentally.

 
Consistent with the two-hybrid interaction data, it was found that RAR1 and SGT1 form a complex in vivo (Azevedo et al., 2002Go). Additionally, SGT1 also is present as a subunit of SCF-type E3 ubiquitin ligase complexes, but these complexes apparently are distinct from the RAR1/SGT1 complex (Figure 1). Because SGT1 is an essential component of SCF-type E3 ubiquitin ligases, it can be assumed that the RAR1/SGT1b complex also functions in protein degradation; this notion is supported by the finding that RAR1 coimmunoprecipitates with components of another protein complex with a role in protein degradation, the COP9 signalosome (Azevedo et al., 2002Go). The COP9 signalosome is a multiprotein complex that has homology with the "lid" subcomplex of the 26S proteasome that was shown recently to interact with SCF-type E3 ubiquitin ligases in plants, animals, and the fission yeast Schizosaccharomyces pombe (Lyapina et al., 2001Go; Schwechheimer and Deng, 2001Go; Schwechheimer et al., 2001Go). Interestingly, the COP9 signalosome appears to be absent from the budding yeast Saccharomyces cerevisiae (Schwechheimer and Deng, 2001Go).

In plants, it has been possible to demonstrate that the COP9 signalosome is a positive regulator of SCFTIR1, an E3 ubiquitin ligase that mediates responses to the phytohormone auxin (Schwechheimer et al., 2001Go; for review, see Kepinsky and Leyser, 2002Go). In addition, the COP9 signalosome also mediates a number of other physiological responses that are likely to be mediated by SCF-type E3 ubiquitin ligases (C. Schwechheimer, G. Serino, and X.-W. Deng, unpublished data). Furthermore, genetic evidence suggests that the COP9 signalosome is essential for the repression of photomorphogenesis in the dark, which is mediated by the putative non-SCF–type E3 ubiquitin ligase COP1 and an associated ubiquitin-conjugating enzyme variant, COP10 (Osterlund et al., 2000Go; Suzuki et al., 2002Go). Thus, the COP9 signalosome may be a central component of multiple processes that are mediated by E3 ubiquitin ligases belonging to different E3 families. Given this information, it is conceivable that RAR1 and SGT1b may participate in a novel E3 complex that also is regulated by the COP9 signalosome.

What could be the possible role of ubiquitylation or protein degradation in the context of pathogen response? At present, the molecular data are insufficient to draw strong conclusions regarding the precise role of RAR1 and SGT1 in defense signaling; however, there are several hypotheses that could be subjected to experimental tests. The most interesting clue for a role of protein degradation comes from the finding that the abundance of the R protein RPM1 declines rapidly after an AVR-induced resistance response (Boyes et al., 1998Go). However, Tornero et al. (2002)Go now report that RPM1 is undetectable in rar1 mutants. This finding cannot be integrated easily into a model in which RAR1 mediates RPM1 protein degradation, and it suggests that RAR1 actually prevents RPM1 degradation in the absence of an AVR-induced stimulus, although this may be an indirect effect. Interestingly, RPM1 protein levels also decrease in the absence of the RIN4 protein, which appears to function with RPM1 in the recognition of its corresponding AVR products (Mackey et al., 2002Go). It will be important to discover whether other R proteins are affected similarly in rar1 or sgt1b mutants.

Another role for RAR1/SGT1b-mediated protein degradation in resistance responses could be to modulate the abundance of positive or negative regulators of pathogen-induced gene expression. Expression of the PR1 gene is constitutive in COP9 signalosome loss-of-function mutants, and this could be attributed to an inability to degrade a transcriptional activator that is degraded normally in the absence of a stimulus (Mayer et al., 1996Go). Interestingly, PR1 also is expressed constitutively in COP1 and DET1 loss-of-function mutants (Mayer et al., 1996Go). Although the molecular role of DET1 is not understood at present, there is strong evidence that COP1 together with the E2 ubiquitin-conjugating enzyme COP10 functions as an E3 ubiquitin ligase (Osterlund et al., 2000Go; Suzuki et al., 2002Go). These findings suggest a potential role for COP1, DET1, and the COP9 signalosome in pathogen responses. It will be interesting to learn whether any of the related mutants are affected in R gene–mediated resistance.

The recent identification of SGT1b loss-of-function mutants also triggers a certain interest in the function of its Arabidopsis homolog SGT1a, for which mutants have not been identified. It will be interesting to determine if SGT1a and SGT1b mediate similar, dissimilar, or overlapping sets of pathogen responses. Given the anticipated wide spectrum of SCF-mediated processes in plants, it also will be interesting to learn about the role and the specificity of SGT1 proteins in other plant responses, such as cell cycle control and auxin response. At this time, no obvious phenotypes unrelated to disease resistance have been associated with the rar1 or sgt1b mutations, but further analysis may reveal more subtle or conditional effects. Given the versatility and ease of manipulation of the resistance response as a model system, the function and specificity of other components of the degradation machinery certainly will be identified soon, along with their degradation substrates. An exciting time lies ahead as new directions open up for dissecting resistance-signaling processes in plants.

References

Aarts, N., Metz, M., Holub, E.B., Staskawicz, B.J., Daniels, M.J., and Parker, J.E. (1998). Different requirements for EDS1 and NDR1 by disease resistance genes define at least two R gene mediated pathways in Arabidopsis. Proc. Natl. Acad. Sci. USA 95, 10306–10311.[Abstract/Free Full Text]

Austin, M.J., Muskett, P., Kahn, K., Feys, B.J., Jones, J.D.G., and Parker, J.E. (2002). Regulatory role of SGT1 in early R gene-mediated plant defenses. Science 295, 2073–2076.[Abstract/Free Full Text]

Azevedo, C., Sadanandom, A., Kitagawa, K., Freialdenhoven, A., Shirasu, K., and Schulze-Lefert, P. (2002). The RAR1 interactor SGT1, an essential component of R gene-triggered disease resistance. Science 295, 2077–2080.[Abstract/Free Full Text]

Bachmair, A., Novatchkova, M., Potuschak, T., and Eisenhaber, F. (2001). Ubiquitylation in plants: A post-genomic look at a post-translational modification. Trends Plant Sci. 6, 463–470.[CrossRef][ISI][Medline]

Bittner-Eddy, P.D., and Beynon, J.L. (2001). The Arabidopsis downy mildew resistance gene, RPP13-Nd, functions independently of NDR1 and EDS1 and does not require the accumulation of salicylic acid. Mol. Plant-Microbe Interact. 14, 416–421.[Medline]

Boyes, D.C., Nam, J., and Dangl, J.L. (1998). The Arabidopsis thaliana RPM1 disease resistance gene product is a peripheral plasma membrane protein that is degraded coincident with the hypersensitive response. Proc. Natl. Acad. Sci. USA 96, 3292–3297.[Abstract/Free Full Text]

Century, K.S., Holub, E.B., and Staskawicz, B.J. (1997). NDR1, a pathogen induced component required for Arabidopsis disease resistance. Science 278, 1963–1965.[Abstract/Free Full Text]

Deshaies, R.J. (1999). SCF and Cullin/RING H2-based ubiquitin ligases. Annu. Rev. Cell Dev. Biol. 15, 435–467.[CrossRef][ISI][Medline]

Dodds, P.N., Lawrence, G.J., and Ellis, J.G. (2001). Six amino acid changes confined to the leucine-rich repeat {beta}-strand/{beta}-turn motif determine the difference between the P and P2 rust resistance specificities in flax. Plant Cell 13, 163–178.[Abstract/Free Full Text]

Ellis, J.G., Lawrence, G.J., Luck, J.E., and Dodds, P.N. (1999). Identification of regions in alleles of the flax rust resistance gene L that determine differences in gene-for-gene specificity. Plant Cell 11, 495–506.[Abstract/Free Full Text]

Feys, F.J., Moisan, L.J., Newman, M.-A., and Parker, J.E. (2001). Direct interaction between the Arabidopsis disease resistance signaling proteins, EDS1 and PAD4. EMBO J. 20, 5400–5411.[CrossRef][ISI][Medline]

Glazebrook, J. (2001). Genes controlling expression of defense responses in Arabidopsis: 2001 status. Curr. Opin. Plant Biol. 13, 1983–1986.

Jia, Y., McAdams, S.A., Bryan, G.T., Hershey, H.P., and Valent, B. (2000). Direct interaction of resistance gene and avirulence gene products confers rice blast resistance. EMBO J. 19, 4004–4014.[CrossRef][ISI][Medline]

Jørgensen, J.H. (1996). Effect of three suppressors on the expression of powdery mildew resistance genes in barley. Genome 39, 492–498.

Kepinsky, S., and Leyser, O. (2002). Ubiquitination and auxin signaling: A degrading story. Plant Cell 14 (suppl.), S81–S95.[Free Full Text]

Kitagawa, K., Skowyra, D., Elledge, S.J., Harper, J.W., and Hieter, P. (1999). SGT1 encodes an essential component of the yeast kinetochore assembly pathway and a novel subunit of the SCF ubiquitin ligase complex. Mol. Cell 4, 21–23.[CrossRef][ISI][Medline]

Lyapina, S., Cope, G., Shevchenko, A., Serino, G., Tsuge, T., Zhou, C., Wolf, D.A., Wei, N., Shevchenko, A., and Deshaies, R.J. (2001). Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalosome. Science 292, 1382–1385.[Abstract/Free Full Text]

Mackey, D., Holt, B.F., III, Wiig, A., and Dangl, J.L. (2002). RIN4 interacts with Pseudomonas syringae type III effector molecules and is required for RPM1-mediated resistance in Arabidopsis. Cell 108, 743–754.[CrossRef][ISI][Medline]

Mayer, R., Raventos, D., and Chua, N.-H. (1996). Det1, cop1 and cop9 mutations cause inappropriate expression of several gene sets. Plant Cell 8, 1951–1959.[Abstract]

McDowell, J.M., Cuzick, A., Can, C., Beynon, J., Dangl, J.L., and Holub, E.B. (2000). Downy mildew (Peronospora parasitica) resistance genes in Arabidopsis vary in functional requirements for NDR1, EDS1, NPR1, and salicylic acid accumulation. Plant J. 22, 523–529.[CrossRef][ISI][Medline]

Muskett, P.R., Kahn, K., Austin, M.J., Moisan, L.J., Sadanandom, A., Shirasu, K., Jones, J.D.G., and Parker, J.E. (2002). Arabidopsis RAR1 exerts rate-limiting control of R gene–mediated defenses against multiple pathogens. Plant Cell 14, 979–992.[Abstract/Free Full Text]

Osterlund, M.T., Hardtke, C.S., Wei, N., and Deng, X.-W. (2000). Targeted destabilization of HY5 during light-regulated development of Arabidopsis. Nature 405, 462–466.[CrossRef][Medline]

Schwechheimer, C., and Deng, X.-W. (2001). COP9 signalosome revisited: A novel mediator of protein degradation. Trends Cell Biol. 11, 420–426.[CrossRef][ISI][Medline]

Schwechheimer, C., Serino, G., Callis, J., Crosby, W.L., Lyapina, S., Deshaies, R.J., Gray, W.M., Estelle, M., and Deng, X.-W. (2001). Interactions of the COP9 signalosome with the E3 ubiquitin ligase SCFTIR1 in mediating auxin response. Science 292, 1379–1382.[Abstract/Free Full Text]

Shirasu, K., Lahaye, T., Tan, M.-W., Zhou, F., Azevedo, C., and Schulze-Lefert, P. (1999). A novel class of eukaryotic zinc-binding proteins is required for disease resistance signaling in barley and development in C. elegans. Cell 99, 355–366.[CrossRef][ISI][Medline]

Suzuki, G., Yanagawa, Y., Kwok, S.F., Matsui, M., and Deng, X.-W. (2002). Arabidopsis COP10 is a ubiquitin-conjugating enzyme variant that acts together with COP1 and the COP9 signalosome in repressing photomorphogenesis. Genes Dev. 16, 554–559.[Abstract/Free Full Text]

Tao, Y., Yuan, F., Leister, R.T., Ausubel, F.M., and Katagiri, F. (2000). Mutational analysis of the Arabidopsis nucleotide binding site–leucine-rich repeat resistance gene RPS2. Plant Cell 12, 2541–2554.[Abstract/Free Full Text]

Tör, M., Gordon, P., Cuzick, A., Eulgem, T., Sinapidou, E., Mert-Türk, F., Can, C., Dangl, J.L., and Holub, E.B. (2002). Arabidopsis SGT1b is required for defense signaling conferred by several downy mildew resistance genes. Plant Cell 14, 993–1003.[Abstract/Free Full Text]

Tornero, P., Merritt, P., Sadanandom, A., Shirasu, K., Innes, R.W., and Dangl, J.L. (2002). RAR1 and NDR1 contribute quantitatively to disease resistance in Arabidopsis, and their relative contributions are dependent on the R gene assayed. Plant Cell 14, 1005–1015.[Abstract/Free Full Text]




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