Supplemental Data

Files in this Data Supplement:

• Supplemental Table 1 - Genes Showing Significant Differential Expression in ppi1.
• Supplemental Figure 1 - DNA Array Analysis of Nucleus-Encoded Chloroplast Genes in ppi1. Total RNA isolated from 10-day-old wild-type and ppi1 mutant seedlings was used to probe a nylon filter DNA array carrying 3292 gene-specific tags (see Figure 4). Signal intensity in ppi1 was plotted against signal intensity in wild type, on a logarithmic scale, for all 1461 genes showing differential expression (see Supplemental Table 1). Each gene is represented by a small grey circle. Additionally, the 50 representative genes listed in Table 2 are highlighted using the indicated symbols. Symbols lying above the solid diagonal line represent genes that are up-regulated in ppi1, and symbols lying below the line represent genes that are down-regulated in ppi1; the dotted lines indicate changes in gene expression of +/-25%.
• Supplemental Figure 2 - Thermolysin Treatment of Protein Import Reactions. In vitro-translated, [35S]methionine-labelled preSSU (A), preOE33 (B), and preL11 (C) were imported into wild-type (WT) and mutant (ppi1) chloroplasts for 15 min; in the case of preL11, only ppi1 chloroplasts were analysed, since no difference between ppi1 and wild type was expected (see Figure 5C). At the end of each import reaction, samples were divided in two; in each case, one-half was treated with the protease, thermolysin (Th; +), and the other half was left untreated (-). Ten percent of the translation product added to each import reaction was loaded as a control (10%). Precursor (p), intermediate (i; OE33 only), and mature (m) protein forms are indicated to the right of each panel.