Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Primary Structure Comparison between SUSIBA2, the Rice and Wheat SUSIBA2 Orthologs, and SPF1. (A) Alignment of the amino acid sequences between the conserved WRKY domains of SUSIBA2, the Rice and Wheat SUSIBA2 Orthologs, and SPF1. (B) Alignment of the entire amino acid sequence of SUSIBA2 with that of the rice SUSIBA2 ortholog. The portion corresponding to the isolated rice cDNA clone is denoted with a hatched double line under the sequences, with green indicating the aligned regions in (A). The “SUSIBA2-specific” sequence (c.f. Figure 2) is indicated by a stretch of blue # signs.
• Supplemental Figure 2 - Schematic Comparison of SUSIBA2 and SUSIBA1. SUSIBA1 and the C-terminal half of SUSIBA2 share >95% amino acid identity over most of their lengths. The exception is the divergent N terminus of SUSIBA1. The conserved WRKY domains and the nuclear localization signals (NLS) are indicated.
• Supplemental Figure 3 - SURE and SURE-like Sequences in Plant Promoters. Alignment of SURE sequences in promoters of plant genes known to be subjected to sugar-induced expression and the WRKY20 gene from rice. Conserved A nucleotides are in bold and the putative consensus core (A^A/_TAA) is highlighted in yellow with the T nucleotides shown in green. SURE-c, SURE-b, SURE-a 3’ and SURE-a 3’ m3 refer to the native or modified SURE sequences of barley iso1 (this work); sbeIIb, barley sbeIIb (Sun et al., 1998); ssI, barley ssI (Gubler et al., 2000); agpaseS, barley agpaseS (Thorbjørnsen et al., 2000); amy, Arabidopsis gene for β-amylase (Mita et al., 1995); sbeI, maize sbeI (Kim and Guiltinan, 1999); sus4, potato gene for sucrose synthase (Fu et al., 1995); vsp, soybean gene for vegetative storage protein (Rhee and Staswick, 1992); PI-II, potato gene for proteinase inhibitor II (Kim et al., 1991); ps20, potato patatin class I gene (Grierson et al., 1994); WRKY20, the rice susiba2 ortholog (this work).