Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - In organello Import and Membrane Insertion of LHCP in csr1-1 Mutant chloroplasts. For detail, see the legend to Figs. 6A and 6B.
• Supplemental Figure 2 - Photosynthetic complexes of the wild-type chloroplasts were analyzed by blue native gel electrophoresis and the following Immunoblotting. For detail, see the legend to Fig. 8.
• Supplemental Figure 3 - Immunoblot Analyses of Components of the Thylakoid Protein Translocation Machineries in csr1 Mutants. (A) Thylakoidal localization of D1, ALB3, and cpSecY in csr1-1 mutant chloroplasts. Chloroplasts isolated from either the wild-type or the csr1-1 mutant were fractionated to yield stroma (Str) and thylakoids (Thy). Thylakoids were further treated with trypsin and analyzed as described in Fig. 2C. * and ** denote characteristic proteolytic fragments derived from the integrated ALB3 and cpSecY, respectively (Woolhead et al., 2001; Laidler et al., 1995). (B) Treatment of wild-type and csr1-1 mutant thylakoids with chaotropic agents. Isolated thylakoids were resuspended in 10 mM HEPES-KOH pH 7 (Mock), 100 mM Na2CO3, or 4 M Urea. After incubation on ice for 30 min, samples were centrifuged at 200,000g for 1 h at 4^oC. Equal fractions of the supernatants (S) and pellets (P) were analyzed by immunoblotting.