| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
|
The Plant Cell 19:3-6 (2007) © 2007 American Society of Plant Biologists
Gibberellins Are Modified by Methylation in PlantaNews and Reviews Editor neckardt{at}aspb.org
Methylation, the attachment of a methyl group in place of a hydrogen atom, is a common modification to numerous biological substrates. Almost all classes of plant metabolite are known to be methylated, including amino acids, alkaloids, phenylpropanoids, sugars, sterols, thiols, flavonoids, and purines (D'Auria et al., 2003
Among the plant MTs identified recently is a family of enzymes that includes OMTs that methylate an oxygen in the carboxyl group of several plant hormones, including salicylic acid (SA), jasmonic acid (JA), and indole-3-acetic acid (IAA). Some of the first genes to be characterized in this family were those encoding SAM:salicylic acid carboxyl MT (SAMT) from Clarkia brewerii (Ross et al., 1999  ), SAM:benzoic acid carboxyl MT (BAMT) from snapdragon (Murfitt et al., 2000), and a theobromine synthase (which is an N-methyltransferase) from coffee (Ogawa et al., 2001). This family was therefore given the name SABATH, incorporating letters from SAMT, BAMT, and theobromine synthase. Additional SABATH enzymes have been identified from Arabidopsis, including a SAM:jasomonic acid carboxyl MT (Seo et al., 2001) and a SAM:IAA carboxyl MT (Zubieta et al., 2003; Qin et al., 2005). The SABATH family in the Arabidopsis genome includes 24 genes, and Eran Pichersky and his colleagues have undertaken the task to determine the functions of all 24 genes (Chen et al., 2003). In this issue of The Plant Cell, Varbanova et al. (pages 32–45) report that two members of the Arabidopsis SABATH methyltransferase gene family, designated GAMT1 and GAMT2, encode SAM-dependent MTs that methylate the 7-carboxyl group of gibberellins (GAs), resulting in the corresponding methyl esters (MeGAs). Both genes were found to be expressed most highly in the siliques during seed development. The authors found that siliques of mutant plants with reduced levels of GAMT1 and GAMT2 showed an increase in concentrations of free GAs, and mature seeds of mutants appeared to have higher concentrations of active GAs, indicating the GAMT activity reduces the level of active GA in these plant tissues. Thus, methylation of GAs may constitute an additional mechanism for modulating cellular GA concentrations in addition to the previously described oxidative pathways.
As a first step, the authors expressed full-length cDNAs of the two genes in Escherichia coli, purified the proteins, and tested their activity with a large number of potential substrates using a high-throughput assay. In this test, both enzymes showed MT activity with GA3, the one GA included in the initial high-throughput assay, and no activity was observed with any other substrates tested. At the time of this writing, 136 GAs have been identified in higher plants, fungi, and bacteria (http://www.plant-hormones.info/gibberellins.htm), but only a small number have been found to be biologically active (e.g., GA1, GA3, GA4, and GA7) (Hedden and Phillips, 2000 ). Varbanova et al. tested numerous additional GAs and found that both enzymes showed activity with all but a few. GAMT1 showed highest activity with GA9 and GA20, followed by GA3 and other GAs, whereas GAMT2 showed highest activity with GA4, followed by GA34, GA9, GA3, and others. The group then conducted experiments using Arabidopsis, tobacco, and petunia plants overexpressing GAMT1 and GAMT2 and found that overexpression of the genes resulted in dwarf phenotypes similar to those exhibited by GA-deficient mutants (see figure ). Measurement of endogenous levels of precursor and bioactive GAs in GAMT-overexpressing Arabidopsis plants just after flowering confirmed that the levels of GA4, the major bioactive form at this stage, as well as a number of precursor GAs, were significantly lower in GAMT-overexpressing plants than in the wild type. They therefore concluded that the dwarfed and semidwarfed phenotypes of plants overexpressing GAMT1 and GAMT2 could be attributed to reduced levels of bioactive GA.
The authors obtained T-DNA insertional mutants of GAMT1 and GAMT2, which were verified as null mutants, and crossed the single mutant lines to obtain the homozygous double mutant. The null mutants all grew normally and had no obvious visible morphological differences compared with wild-type plants. The levels of bioactive GA4 and GA1 were measured and found to be significantly higher in siliques of the gamt1 gamt2 double mutant than in wild-type plants. Analysis of gene expression of GAMT1 and GAMT2 in wild-type plants showed that both genes were highly expressed in siliques and not in any other tissue examined (e.g., leaves, stems, flowers, and roots), except for weak but detectable levels in germinating seeds. These results supported the conclusion that GAs are endogenous substrates for GAMT1 and GAMT2 in wild-type siliques and suggest that methylation of GA plays a role in or has a function during seed development.
GA is known to function in promoting seed germination, but its role in early seed development is unclear. Many plants accumulate high levels of GA during seed development, and in many cases, the GAs produced are inactive forms that exhibit a range of unusual modifications (a large number of the 136 known GAs are found in seeds). It remains unclear what role GAs play during seed development per se, although they could function in promoting growth and development of the early embryo (Finkelstein, 2004 ). However, it appears essential that bioactive forms of GA are removed or inactivated during seed maturation; otherwise, they could affect seed dormancy, as well as germination and early seedling growth. For example, the phenotype of the slender mutant of pea is due to loss of GA2 oxidase activity, resulting in the accumulation of GA20 in the seed, which upon germination is converted to GA1 and results in an abnormally elongated seedling (Lester et al., 1999; Martin et al., 1999). To examine the effect of GA methylation on Arabidopsis seed germination, Varbanova et al. placed homozygous single and double gamt1 gamt2 mutant seeds on medium supplemented with ancymidol, an inhibitor of GA biosynthesis. Germination was inhibited by ancymidol to a lesser extent in the mutant seed compared with wild-type seed and to a greater extent in seed of plants overexpressing GAMT1 and GAMT2 compared with the wild type. This suggests that an important function of GAMTs in seed is in the deactivation or removal of active GA during seed maturation.
Methyl esters are more hydrophobic than the free acid form of a compound and as a result can more easily pass through membranes and are more highly mobile in planta. For example, MeIAA and MeJA are often used experimentally because, due to their higher rate of mobility, a given concentration applied exogenously is more potent than the same concentration of the corresponding free acid. It has been widely assumed that after the methylated hormone enters a cell, it is hydrolyzed and the free acid is the active form. MeSA and MeJA have been found in many plants and are thought to function as volatile signals that function in plant defense (e.g., Shulaev et al., 1997 ; Baldwin et al., 2006). However, it is believed that the free acids of SA and JA are released inside the cell and function as the active compounds in eliciting defense response pathways. Until recently, however, there has been very little study of the hydrolysis of these methylated hormones.
Forouhar et al. (2005)
Unlike MeJA and MeIAA, MeGAs are not highly volatile and they do not appear to have activity when applied exogenously. The work of Varbanova et al. suggests that methylation of GA is an irreversible process in planta. The authors attempted to measure endogenous MeGAs in siliques of wild-type plants and found them to be undetectable, although a range of internal standards added to extracts could be detected. These results might indicate that MeGAs are rapidly degraded or converted to other compounds. However, this question needs to be examined more thoroughly before a definitive conclusion can be reached. Interestingly, Varbanova et al. note that the recently identified GID1 family of GA receptors (Ueguchi-Tanaka et al., 2005
The major route known for GA catabolism is 2ß-hydroxylation, which is catalyzed by GA 2-oxidases and results in biologically inactive products that cannot be converted back to active forms (Thomas et al., 1999 In addition, the work of Varbanova et al. provides an excellent example of the utility of functional genomics and the importance of the Arabidopsis model system in plant biology. Starting from genome sequence, the authors used high-throughput methods in conjunction with all of the available Arabidopsis tools and resources to identify the function of genes, the activity of gene products, and the possible biological roles of the GA methyl transferases, which had remained elusive to conventional physiological, biochemical, or genetic approaches. Varbanova et al. have shown that GAMT1 and GAMT2 function in GA homeostasis in developing siliques of Arabidopsis. The authors suggest that methylation of GAs by GAMT1 and GAMT2 serves to deactivate GAs and initiate their degradation in developing seed. This work represents a significant step forward in arriving at a complete understanding of GA metabolism and GA-related processes in plant development.
www.plantcell.org/cgi/doi/10.1105/tpc.107.050955
Baldwin, I.T., Halitschke, R., Paschold, A., von Dahl, C.C., and Preston, C.A. (2006). Volatile signaling in plant-plant interactions "Talking Trees" in the genomics era. Science 311: 812–815. Chen, F., D'Auria, J.C., Tholl, D., Ross, J.R., Gershenzon, J., Noel, J.P., and Pichersky, E. (2003). An Arabidopsis thaliana gene for methylsalicylate biosynthesis, identified by a biochemical genomics approach, has a role in defense. Plant J. 36: 577–588.[CrossRef][ISI][Medline] D'Auria, J.C., Chen, F., and Pichersky, E. (2003). The SABATH family of methyltransferases in Arabidopsis thaliana and other plant species. In Recent Advances in Phytochemistry, J. Romeo, ed (Oxford, UK: Elsevier Science), pp. 253–283. Elliott, R.C., Smith, J.L., Lester, D.R., and Reid, J.B. (2001). Feedforward regulation of gibberellin deactivation in pea. J. Plant Growth Regul. 20: 87–94.[CrossRef][ISI] Finkelstein, R.R. (2004). The role of hormones during seed development and germination. In Plant Hormones: Biosynthesis, Signal Transduction, Action! P.J. Davies, ed (Dordrecht, The Netherlands: Kluwer Academic Publishers), pp. 513–537. Forouhar, F., Yang, Y., Kumar, D., Chen, Y., Fridman, E., Park, S.W., Chiang, Y., Action, T.B., Montelione, G.T., Pichersky, E., Klessig, D.F., and Tong, L. (2005). Structural and biochemical studies identify tobacco SABP2 as a methyl salicylate esterase and implicate it in plant immunity. Proc. Natl. Acad. Sci. USA 102: 1773–1778. Griffiths, J., Murase, K., Rieu, I., Zentella, R., Zhang, Z.-L., Powers, S.J., Gong, F., Phillips, A.L., Hedden, P., Sun, T.-p., and Thomas, S.G. (2006). Genetic characterization and functional analysis of the GID1 gibberellin receptors in Arabidopsis. Plant Cell 18: 3399–3414. Hedden, P., and Phillips, A.L. (2000). Gibberellin metabolism: New insights revealed by the genes. Trends Plant Sci. 5: 523–530.[CrossRef][ISI][Medline] Ibrahim, R.K., Bruneau, A., and Bantignies, B. (1998). Plant O-methyltransferases: Molecular analysis, common signature and classification. Plant Mol. Biol. 36: 1–10.[CrossRef][ISI][Medline] Kumar, D., Gustafsson, C., and Klessig, D.F. (2006). Validation of RNAi silencing specificity using synthetic genes: Salicylic acid-binding protein 2 is required for innate immunity in plants. Plant J. 45: 863–868.[CrossRef][ISI][Medline] Lester, D.R., Ross, J.J., Smith, J.J., Elliott, R.C., and Reid, J.B. (1999). Gibberellin 2-oxidation and the SLN gene of Pisum sativum. Plant J. 19: 65–73.[CrossRef][ISI][Medline] Martin, D.N., Proebsting, W.M., and Hedden, P. (1999). The SLENDER gene of pea encodes a gibberellin 2-oxidase. Plant Physiol. 121: 775–781. Murfitt, L.M., Kolosova, N., Mann, C.J., and Dudareva, N. (2000). Purification and characterization of S-adenosyl-L-methionine:benzoic acid carboxyl methyltransferase, the enzyme responsible for biosynthesis of the volatile ester methyl benzoate in flowers of Antirrhinum majus. Arch. Biochem. Biophys. 382: 145–151.[CrossRef][ISI][Medline] Ogawa, M., Herai, Y., Koizumi, N., Kusano, T., and Sano, H. (2001). 7-Methylxanthine methyltransferase of coffee plants. Gene isolation and enzymatic properties. J. Biol. Chem. 276: 8213–8218. Qin, G., Gu, H., Zhao, Y., Ma, Z., Shi, G., Yang, Y., Pichersky, E., Chen, H., Lui, M., Chen, Z., and Qu, L.-J. (2005). Regulation of auxin homeostasis and plant development by an indole-3-acetic acid carboxyl methyltransferase in Arabidopsis. Plant Cell 17: 2693–2704. Ross, J.R., Nam, K.H., D'Auria, J.C., and Pichersky, E. (1999). S-adenosyl-L-methionine:salicylic acid carboxyl methyltransferase, an enzyme involved in floral scent production and plant defense, represents a new class of plant methyltransferases. Arch. Biochem. Biophys. 367: 9–16.[CrossRef][ISI][Medline] Schneider, G., Jensen, E., Spray, C.R., and Phinney, B.O. (1992). Hydrolysis and reconjugation of gibberellin A20 glucosyl ester by seedlings of Zea mays L. Proc. Natl. Acad. Sci. USA 89: 8045–8048. Schomburg, F.M., Bizzell, C.M., Lee, D.L., Zeevaart, J.A.D., and Amasino, R.M. (2003). Overexpression of a novel class of gibberellin 2-oxidases decreases gibberellin levels and creates dwarf plants. Plant Cell 15: 151–163. Seo, H.S., Song, J.T., Cheong, J.J., Lee, Y.H., Lee, Y.W., Hwang, I., Lee, J.S., and Choi, Y.D. (2001). Jasmonic acid carboxyl methyltransferase: A key enzyme for jasmonate-regulated plant responses. Proc. Natl. Acad. Sci. USA 98: 4788–4793. Shulaev, V., Silverman, P., and Raskin, I. (1997). Airborne signalling by methyl salicylate in plant pathogen resistance. Nature 385: 718–721.[CrossRef] Stuhlfelder, C., Mueller, M.J., and Warzecha, H. (2004). Cloning and expression of a tomato cDNA encoding a methyl jasmonate cleaving esterase. Eur. J. Biochem. 271: 2976–2983.[ISI][Medline] Thomas, S.G., Phillips, A.L., and Hedden, P. (1999). Molecular cloning and functional expression of gibberellin 2-oxidases, multifunctional enzymes involved in gibberellin deactivation. Proc. Natl. Acad. Sci. USA 96: 4698–4703. Ueguchi-Tanaka, M., Ashikari, M., Nakajima, M., Itoh, H., Katoh, E., Kobayashi, M., Chow, T.Y., Hsing, Y.I., Kitano, H., Yamaguchi, I., and Matsuoka, M. (2005). GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin. Nature 437: 693–698.[CrossRef][Medline] Varbanova, M., et al. (2007). Methylation of gibberellins by Arabidopsis GAMT1 and GAMT2. Plant Cell 19: 32–45. Zhu, Y., et al. (2006). ELONGATED UPPERMOST INTERNODE encodes a cytochrome P450 monooxygenase that epoxidizes gibberellins in a novel deactivation reaction in rice. Plant Cell 18: 442–456. Zubieta, C., Koscheski, P., Ross, J.R., Yang, Y., Pichersky, E., and Noel, J.P. (2003). Structural basis for substrate recognition in the salicylic acid carboxyl methyltransferase family. Plant Cell 15: 1704–1716. Related articles in Plant Cell:
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| ASPB Publications | THE PLANT CELL | PLANT PHYSIOLOGY | |
|---|---|---|---|
TOP
SABATH METHYLTRANSFERASES ACT ON...
GAMTs AFFECT GA HOMEOSTASIS...