Supplemental Data

Files in this Data Supplement:

• Video Sequence 1 - Tethering complexes. This movie shows a series of tomographic slices through two tethered vesicles with an L-shaped complex (upper vesicle) and an exocyst-like Y-shaped complex (arrows).
• Video Sequence 2 - Tubulo-vesicular network (TVN) cell plate. This movie corresponds to a model of a TVN cell plate rotated 360^o, where the 3-D arrangement of the cell plate, phragmoplast MTs, and CPAM is clearly shown.
• Video Sequence 3 - Tubular network (TN). This movie shows a TN cell plate where vesicular domains are almost absent. Note the lower density of MTs and their greater distance from the cell plate.
• Video Sequence 4 - Peripheral growth zone (PGZ) / Planar fenestrated sheet (PFS). In this movie, a PGZ domain (left) is seen fused with the plasma membrane. Most of the MTs and vesicles are concentrated around the PGZ. The PFS (center-right) is almost devoid of MTs and vesicles, which are focused only to the large fenestrae. Mitochondria, Golgi stacks, and vacuoles are present near the cell plate. Note the increase in the number of clathrin-coated vesicles with respect to the previous stages.