Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Comparison of independent Genechip hybridizations. Scatter plots comparing the raw signal intensities of three independent experiments from control-treated guard cells ([A] and [B]) and ABA-treated guard cells ([C] and [D]). Each gene is represented by one dot. For each gene, the raw RNA expression level in one experiment is given on the x axis and the expression level for the same gene in the other experiment is plotted on the y axis. The solid diagonal lines indicate a difference by a factor of 2 between the two hybridizations for visual reference purposes. Some points show a slight off-center distribution, which may be attributable to direct use of raw data comparing independent experiments without normalization. Significantly expressed genes detected as present (P) or marginal (M) in two or three samples are represented by black dots, whereas genes for which expression levels were not significant in two or three samples are illustrated as gray dots.
• Supplemental Figure 2 - RT-PCR analyses confirm results obtained from chip hybridization experiments. RT-PCR was performed using guard cell and mesophyll cell RNA with primers for selected genes from guard cell preferential genes showing no ABA modulation (glycosyl hydrolase [At4g24024], Ser-Thr protein kinase [At2g32850]), from mesophyll cell preferential genes showing no ABA modulation (protein kinase [At1g14000]), and from Figure 5-derived group I (transcription factor [At2g46680], cold acclimation protein [At2g15970]), group II (MAP kinase [At1g05100], 14-3-3 protein [At5g10450], unknown protein [At4g24130], aconitate hydratase [At4g35830]), group III (chlorophyll a/b binding protein [At3g47470], plasma membrane intrinsic protein [At3g61430], DNA-binding protein), group IV (GASA4 [At5g15230]), group V (chloride channel [At5g40890], membrane channel protein [At2g28900], two transcription factors [At2g21650; At1g08810]), and group VI (Rubisco binding protein [At2g28000], PEP carboxylase [At2g42600], brassinosteroid receptor kinase [At3g13380]). Results are from 27 RT-PCR cycles. Actin2 gene was used as control. GC, guard cells; GC + ABA, guard cells treated with ABA; MC, mesophyll cells; MC + ABA, mesophyll cells treated with ABA.
• Supplemental Table 1 - See Supplemental Table 1-Revised
• Supplemental Table 2
• Supplemental Table 3
• Supplemental Table 4 - See Supplemental Table 4-Revised
• Supplemental Table 1-Revised - This table was revised. Correction published in The Plant Cell, Vol. 17 No. 4, April 2005.
• Supplemental Table 4-Revised - This table was revised. Correction published in The Plant Cell, Vol. 17 No. 4, April 2005.